Full-automatic sample adding, hybridization and detection method for HPV genotyping chip
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A technology of genotyping and detection methods, applied in biochemical equipment and methods, microbiological measurement/inspection, biochemical instruments, etc., can solve the problems of heavy workload of manual intervention, achieve small workload, ensure continuity, improve The effect of detection efficiency
Pending Publication Date: 2020-03-17
FUJIAN UNIV OF TECH
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[0003] At present, for the HPV genotyping chip of PCR-reverse dot hybridization method, the method of sample extraction-PCR amplification-c
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Embodiment 1
[0044] A detection system for automatic sample loading, hybridization and detection methods of an HPV genotyping chip includes: a PCR product transfer plate, a pipetting device, a biochemical hybridization device, and an image acquisition device;
[0045] The turntable in the PCR product can rotate clockwise or counterclockwise around the axis, and a small test tube is embedded in the circumference of the turntable;
[0046] The pipetting device can rotate around the axis, and the pipetting needle can be lifted up and down vertically to absorb or release a certain solution. In addition, the pipetting device has an automatic cleaning function and can continuously add different liquids;
[0047]The biochemical hybridization device is also a turntable device that can rotate clockwise and counterclockwise. The circumference of the turntable is embedded with large test tubes. At the same time, the large turntable for biochemical hybridization has the function of raising and lowering...
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Abstract
The invention relates to the field of in-vitro diagnostic reagent detection, in particular to a full-automatic sample adding, hybridization and detection method for an HPV genotyping chip. The methodcomprises the following steps: dropwise adding a PCR product into a small test tube of a PCR product middle rotating disc, placing an HPV genotyping chip in a large test tube of a biochemical hybridization large rotating disc, preparing a reaction liquid required by hybridization color development and distilled water for cleaning a pipetting device, and carrying out hybridization detection. The detection method has the beneficial effects that rapid and convenient suction and sample adding are realized, the continuity of detection steps is ensured, the workload of manual intervention is relatively small and the detection efficiency is improved.
Description
technical field [0001] The invention relates to the field of detection of in vitro diagnostic reagents, and more specifically relates to a method for automatic sampling, hybridization and detection of an HPV genotyping chip. Background technique [0002] At present, the main methods for domestic clinical detection of human papillomavirus (Human papillomavirus, HPV) DNA are real-time fluorescent PCR and hybrid capture method. Detect the result of hybridization between the amplified product and the coated type-specific probe membrane, and then use the alkaline phosphatase system for qualitative detection, so that 21 HPV genotypes (6, 11, 16, 18, 31, 33, 35, 39 , 42, 43, 44, 45, 51, 52, 53, 56, 58, 59, 66, 68 and CP8304) for typing detection and analysis, which has important clinical application value for preventing cervical cancer. [0003] At present, for the HPV genotyping chip of the PCR-reverse dot hybridization method, the method of sample extraction-PCR amplification-co...
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