Application of Monascinol in preparation of fat-reducing product
A technology for monasflavin and its products, which is applied in the application field of monasflavin C in the preparation of fat-reducing products, can solve problems such as soaring risk of disease, and achieve the effects of controlling weight gain, reducing blood lipids, and reducing cytotoxicity
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Embodiment 1
[0066] The cytotoxicity of embodiment 1 monascus xanthin C
[0067] 1) Modeling HepG2 cells with oleic acid
[0068] Take HepG2 cells in good growth state to 2*10 5 cells / mL, 1 mL per well was inoculated in a 6-well culture plate. When the cell adhesion rate reaches 80%-90%, discard the medium. Set up blank group and model group, add complete medium to the blank group, add 0.3mmol / L oleic acid induction solution diluted with complete medium to the model group, add 2mL to each well, and after culturing for 24h, observe the concentration in the cells by Oil Red O staining. Lipid droplet accumulation.
[0069] The cells in the blank group were spindle-shaped, with clear cell edges, and no obvious accumulation of lipid droplets in the cells; the adherent HepG2 cells added with 2 mL0.3mmol / L oleic acid induction solution had no obvious proliferation damage after incubation for 24 hours , and compared with the blank group, it can be seen that there are obvious red lipid droplets...
Embodiment 2
[0077]Example 2 Effect of different dosages of monascus flavin C on cell triglyceride content
[0078] Experiment as follows:
[0079] a. Select well-growing HepG2 cells and press 2*10 5 cells / mL, 1 mL per well was inoculated in a 6-well culture plate and cultured for 12 h, and the medium was discarded after the cells adhered to the wall.
[0080] b. After discarding the culture medium, set up a blank group, a modeling group, and a drug-dosing group.
[0081] Blank control group: add 2 mL of complete medium;
[0082] Oleic acid modeling group: add 2 mL of complete medium containing 0.3 mmol / L oleic acid;
[0083] MC low-dose group: add 2 mL of complete medium containing 0.3 mmol / L oleic acid and 2 μg / mL monascus xanthin C;
[0084] MC medium dose group: add 2 mL of complete medium containing 0.3 mmol / L oleic acid and 4 μg / mL monascus xanthin C;
[0085] MC high-dose group: 2 mL of complete medium containing 0.3 mmol / L oleic acid and 8 μg / mL monascus xanthin C was added. ...
Embodiment 3
[0089] Example 3 Effects of Monascinol (MC for short) and Monascin (MS for short) on the triglyceride content of HepG2 cells modeled with oleic acid
[0090] Experiment as follows:
[0091] Firstly, normal HepG2 cells were obtained, and HepG2 cells (oleic acid concentration: 0.3 mmol / L) after modeling with oleic acid were obtained according to the method in Example 1.
[0092] On the first day, blank control group: HepG2 cells were normally cultured without treatment; oleic acid modeling group (oleic acid concentration 0.3mmol / L), HepG2 cells after oleic acid modeling were not treated; MC group: 2 μg / mL monascus xanthin C (MC) was administered to HepG2 cells after oleic acid modeling; MS group: 2 μg / mL monascus MS was administered to HepG2 cells after oleic acid modeling.
[0093] After completing the administration, continue to cultivate the cells of each group for 24 hours, collect the cells of each group, and use the triglyceride kit (Beijing Pulilai Gene Technology Co., L...
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