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Method for extracting grease from chlamydomonas

A technology for extracting oil and Chlamydomonas, applied in microorganism-based methods, fat oil/fat production, fat oil/fat refining, etc., to achieve the effect of good market application value

Inactive Publication Date: 2020-03-24
沈兰兰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because light intensity and photoperiod are easier to adjust, previous studies on the effect of light on oil accumulation in microalgae mainly focused on light intensity and photoperiod, while there were few studies on the effects of different light qualities on oil accumulation in microalgae

Method used

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  • Method for extracting grease from chlamydomonas
  • Method for extracting grease from chlamydomonas
  • Method for extracting grease from chlamydomonas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting oil from Chlamydomonas comprises the following steps:

[0023] Step 1: Cultivation of Chlamydomonas: Inoculate the activated fresh microalgae culture solution cultivated to the logarithmic growth phase into the Erlenmeyer flask containing freshly prepared microalgae medium according to the inoculum size of 10% (v / v) , wrap the mouth of the bottle with 4 layers of gauze, and place the Erlenmeyer flask in a light incubator for cultivation;

[0024] Step 2: Preparation of dry microalgae powder: measure the volume of the Chlamydomonas culture solution cultivated in Step 1, place it in a centrifuge tube and centrifuge for the first time to obtain the first Chlamydomonas algae sediment and the first supernatant, discard The first supernatant; wash the first Chlamydomonas algae precipitate with distilled water, perform a second centrifugation to obtain the second Chlamydomonas algae sediment and the second supernatant, and collect the second Chlamydomon...

Embodiment 2

[0036] A method for extracting oil from Chlamydomonas comprises the following steps:

[0037] Step 1: Cultivation of Chlamydomonas: Inoculate the activated fresh microalgae culture solution cultivated to the logarithmic growth phase into the Erlenmeyer flask containing freshly prepared microalgae medium according to the inoculum size of 10% (v / v) , wrap the mouth of the bottle with 4 layers of gauze, and place the Erlenmeyer flask in a light incubator for cultivation;

[0038] Step 2: Preparation of dry microalgae powder: measure the volume of the Chlamydomonas culture solution cultivated in Step 1, place it in a centrifuge tube and centrifuge for the first time to obtain the first Chlamydomonas algae sediment and the first supernatant, discard The first supernatant; wash the first Chlamydomonas algae precipitate with distilled water, perform a second centrifugation to obtain the second Chlamydomonas algae sediment and the second supernatant, and collect the second Chlamydomon...

Embodiment 3

[0050] A method for extracting oil from Chlamydomonas comprises the following steps:

[0051] Step 1: Cultivation of Chlamydomonas: Inoculate the activated fresh microalgae culture solution cultivated to the logarithmic growth phase into the Erlenmeyer flask containing freshly prepared microalgae medium according to the inoculum size of 10% (v / v) , wrap the mouth of the bottle with 4 layers of gauze, and place the Erlenmeyer flask in a light incubator for cultivation;

[0052] Step 2: Preparation of dry microalgae powder: measure the volume of the Chlamydomonas culture solution cultivated in Step 1, place it in a centrifuge tube and centrifuge for the first time to obtain the first Chlamydomonas algae sediment and the first supernatant, discard The first supernatant; wash the first Chlamydomonas algae precipitate with distilled water, perform a second centrifugation to obtain the second Chlamydomonas algae sediment and the second supernatant, and collect the second Chlamydomon...

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Abstract

The invention discloses a method for extracting grease from chlamydomonas. The method comprises the following steps: culturing the chlamydomonas, preparing dry microalga powder and extracting the grease. The accumulation of grease in the chlamydomonas is remarkably improved under the interaction of red light and blue light, the higher the blue light proportion is, the more the grease is accumulated, and the accumulation amount of the grease under the red light and blue light (1:2) reaches the highest value. The grease can be used for preparing biodiesel with good quality, and has good market application values.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, in particular to a method for extracting oil from Chlamydomonas. Background technique [0002] The supply of global energy mainly depends on fossil resources such as oil, natural gas and coal. With the continuous development of the economy, the demand and consumption of fossil energy are increasing rapidly. The energy crisis has become a global problem, and the development of alternative energy is imminent. In addition, a large amount of pollutants emitted after the combustion of fossil fuels will have adverse effects on human health and the environment. Compared with fossil energy, biodiesel has the advantages of high flash point, high kinematic viscosity, less environmental pollution, and renewable, and is a very potential alternative energy source. Due to the advantages of high photosynthetic efficiency, short production cycle, high oil content, and no seasonal and climatic ...

Claims

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Application Information

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IPC IPC(8): C11B1/10C11B3/16C12N1/12C12R1/89
CPCC11B1/10C11B3/16C12N1/12Y02E50/10
Inventor 沈兰兰
Owner 沈兰兰
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