Preparation method of astragalus mushroom protein source anti-oxidativepeptide

An antioxidant peptide and protein source technology, applied in the field of Beiqi mushroom protein, can solve the problems of poor processing conditions, affecting the research and development of Beiqi mushroom protein deep processing, and unsatisfactory DPPH free radical scavenging rate.

Inactive Publication Date: 2020-03-24
SHANXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0003] Currently, there is a lack of a process for preparing antioxidant peptides from the protein source of Beiqi mushroom. The DPPH free radical scavenging rate of the antioxidant peptide in the traditional preparation method is not ideal, and the processing conditions are poor, which affects the deep processing of Beiqi mushroom protein. research and development

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  • Preparation method of astragalus mushroom protein source anti-oxidativepeptide
  • Preparation method of astragalus mushroom protein source anti-oxidativepeptide
  • Preparation method of astragalus mushroom protein source anti-oxidativepeptide

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Embodiment Construction

[0079] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0080] see Figure 1-19 :

[0081] A preparation method of protein source antioxidant peptide of Beiqi mushroom, comprising the following steps:

[0082] S1: Alkali-soluble acid precipitation method to extract Beiqi mushroom protein, the specific steps are as follows:

[0083] 1) Put the dried Beiqi mushroom into a grinder and grind it into powder, weigh 5g of Beiqi mushroom dry powder, add 60ml of distilled water to fully dissolve in a 100ml test tube, and adjust the pH value with 0.5mol / L sodium hydroxide solution Adjust to 8.0;

[0084] 2) Transfer the solution into two 50ml centrifuge tubes of the same specific...

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Abstract

The invention discloses a preparation method of an astragalus mushroom protein source anti-oxidative peptide. The method comprises the following steps: S1, extraction of astragalus mushroom protein byan alkali dissolution and acid precipitation method; S2, enzymolysis of the astragalus mushroom protein; S3, screening of proteases; S4, selecting of an enzyme adding mode; S5, selecting of an enzymeadding proportion; S6, single-factor test; S7, response surface testing; S8, determining of DPPH free radical scavenging rate; S9, determining of hydrolysis degree; S10, ABTS and clearance rate testing; S11, total reducing force test; and S12, analytical calculations. According to the invention, the anti-oxidative polypeptide is efficiently obtained in a mode of compounding two proteases by taking the astragalus mushroom protein as a raw material, the optimal process conditions for preparing the astragalus mushroom protein anti-oxidative polypeptide are explored, and a basis is provided for deep processing of the astragalus mushroom protein and development of the anti-oxidative peptide.

Description

technical field [0001] The invention relates to the technical field of Beiqi mushroom protein, in particular to a method for preparing an antioxidant peptide derived from Beiqi mushroom protein. Background technique [0002] Beiqi mushroom contains most amino acids, proteins, organic acids, sugars, alkaloids, phytosterols, flavonoids and glycosides, triterpenoids, etc. Beiqi mushroom contains protein source antioxidant peptides, which are An active peptide in living organisms. In organisms, through various chemical reactions, proteins are hydrolyzed to obtain various polypeptides, and some polypeptides have antioxidant effects, which can reduce the number of free radicals and reduce the impact of free radicals on the body. Antioxidant peptides are highly safe, harmless to the human body, have strong functionality, and can effectively prevent lipid oxidation. After research, free radicals may cause aging and death of cells, and then lead to pigmentation, wrinkles and certai...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 秦楠刘星张朔生缪文玉郭丽丽王小敏
Owner SHANXI UNIV OF CHINESE MEDICINE
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