Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of genotyping and chip detection, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of heavy manual intervention and achieve the effect of ensuring continuity and efficient automatic detection
Pending Publication Date: 2020-03-24
FUJIAN UNIV OF TECH
View PDF6 Cites 1 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0003] At present, for the HPV genotyping chip of PCR-reverse dot hybridization method, the method of sample extraction-PCR amplification-c
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0053] A detection system for a fluidic HPV genotyping chip detection method includes: a pipetting device 1, a temperature-controlled fluidic hybridization platform 2, and an image acquisition device;
[0054] The pipette needle can rotate around the axis, and the pipette needle 11 can be lifted up and down vertically to absorb or release a certain solution. In addition, the pipette needle has an automatic cleaning function, which can continuously add different liquids required for biochemical detection and display reactions ;
[0055] The temperature-controlled and fluid-controlled hybridization platform has a fluid-controlled circulation pump system and temperature control functions. At the same time, a rectangular HPV chip hybridization tank is opened on the surface of the table. The HPV detection chip membrane strip can be placed horizontally in the hybridization tank, and there is a liquid inlet. and drainage holes;
[0056] The image acquisition device includes a video ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to the field of in vitro diagnostic reagent detection, more specifically, relates to a fluidic HPV genotyping chip detection method comprising the following steps: dropwise adding a PCR product of a sample to be detected into PCR to-be-detected liquid small test tubes of an instrument, placing an HPV genotyping chip in an HPV chip hybridization tank, preparing reaction liquidrequired by hybridization, coupling and color development and distilled water required by cleaning, and carrying out automatic detection. The beneficial effects of the invention are that: a biochemical reaction model in a flow control mode is adopted; under the cooperation of a pipetting device for vertical sample adding and a circulating pump, the processes of multi-step hybridization, coupling,color development and cleaning required by HPV detection can share one set of pipetting device, storage pool, fluid pipeline, circulating pump and biochemical reaction tank, so that the continuity ofbiochemical detection steps is ensured, and automatic detection is easier to realize.
Description
technical field [0001] The invention relates to the field of detection of in vitro diagnostic reagents, in particular to a fluidic HPV genotyping chip detection method. Background technique [0002] At present, the main methods for domestic clinical detection of human papillomavirus (Human papillomavirus, HPV) DNA are real-time fluorescent PCR and hybrid capture method. Detect the result of hybridization between the amplified product and the coated type-specific probe membrane, and then use the alkaline phosphatase system for qualitative detection, so that 21 HPV genotypes (6, 11, 16, 18, 31, 33, 35, 39 , 42, 43, 44, 45, 51, 52, 53, 56, 58, 59, 66, 68 and CP8304) for typing detection and analysis, which has important clinical application value for preventing cervical cancer. [0003] At present, for the HPV genotyping chip of the PCR-reverse dot hybridization method, the method of sample extraction-PCR amplification-constant temperature hybridization-visible light interpret...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more