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Cell culture device

A cell culture and cell technology, applied in tissue cell/virus culture devices, animal cells, vertebrate cells, etc., can solve the problems of low efficiency, difficulty in oxygenation, and inability to return the culture solution to the first chamber 810, etc. The effect of simple construction

Active Publication Date: 2020-03-24
FULLSTEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] However, in the above-mentioned cell culture device, since the second chamber 830 has a bellows shape, the cells moved into the second chamber 830 enter the part of the bellows shape protruding toward the outside, and then it is difficult to be received by the second chamber 830. Oxygenation may become difficult due to compression and non-compression effects on the up and down movement of the culture solution
Moreover, regarding the second chamber 830, especially the central part, even if it is a bellows shape in a compressed state, it cannot completely send the culture solution back to the first chamber 810 due to its structure.
Therefore, in order to inoculate the cells on the porous scaffold 820, the cell suspension should be put into the first chamber 810 to stand still or the bellows shape should be compressed and uncompressed to allow the cells to adhere, but due to the dead space and dead space generated capacity, there will be a non-adherent cell suspension, and the efficiency will be lower

Method used

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Examples

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Embodiment 1

[0049] The disc-shaped body arranged in the hollow space portion of the cell culture container is positioned near the lower portion of the cell culture container. The scaffold, the medium, and the cells to be cultured are put into the space of the cell culture container from the opening at the upper end of the cell culture container. Cells are 5.0 x 10 7 adipose-derived mesenchymal stem cells (ADSCs). The scaffold used 5000 pieces of BioNOC II matrix (3D culture) from CESCO. The stroke length of the up and down movement of the annular body is 60 mm, and the speed of the upward or downward movement of the annular body is set at 1.0 mm / sec. The cycle of the up and down movement of the annular body was set to 1.0 times / hour. The number of culture days is eight days.

[0050] Figure 4 It is a graph showing the sugar consumption (mg / dL / day) of the cells per day during the culture days. The glucose consumption of the cells was measured with a GlucCell portable glucose detecto...

Embodiment 2

[0056]In Example 1, the cells were ADSCs, but in Example 2, an attempt was made to culture bone marrow-derived mesenchymal stem cells (BMSCs).

[0057] The disc-shaped body arranged in the hollow space portion of the cell culture container is positioned near the lower portion of the cell culture container. The scaffold, the medium, and the cells to be cultured are put into the space of the cell culture container from the opening at the upper end of the cell culture container. Cells are 3.0 x 10 7 BMSC. The scaffold used 2000 pieces of BioNOC II matrix (3D culture) from CESCO. The stroke length of the up and down movement of the annular body is 40 mm, and the speed of the upward or downward movement of the annular body is set at 1.0 mm / sec. The cycle of the up and down movement of the annular body was set to 1.0 times / hour. The number of culture days is six days.

[0058] Figure 6 It is a graph showing the sugar consumption (mg / dL / day) of the cells per day during the cul...

Embodiment 3

[0064] Because synovium-derived mesenchymal stem cells have very high cartilage differentiation potential and proliferation potential, they are useful for regeneration of cartilage and meniscus. In Example 3, an attempt was made to culture synovium-derived mesenchymal stem cells.

[0065] The disc-shaped body arranged in the hollow space portion of the cell culture container is positioned near the lower portion of the cell culture container. The scaffold, the medium, and the cells to be cultured are put into the space of the cell culture container from the opening at the upper end of the cell culture container. Cells are 1.5 x 10 7 synovium-derived mesenchymal stem cells. The scaffold used 3000 pieces of BioNOCII matrix (3D culture) from CESCO. The stroke length of the up and down movement of the annular body is 45 mm, and the speed of the upward or downward movement of the annular body is set at 1.0 mm / sec. The cycle of the up and down movement of the annular body was set...

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Abstract

The present invention provides a cell culture device that has a simple structure and ensures sufficient nutrient supply to cells and oxygenation of the cells to thereby enable mass cell culture. The cell culture device comprises: a cell culture container which is an approximately cylindrical body provided with a flat bottom part at the lower end; a dish-shaped body having an approximately disc shape which is provided with a plurality of magnetic attraction members, said magnetic attraction members being positioned in the circumferential part at equal intervals, and horizontally disposed in a non-contact state within a hollow space inside the cell culture container; and a cyclic body which is provided with a plurality of magnetic attraction members and positioned outside the cell culture container so that the cell culture container is located within the cycle thereof. The cyclic body and the dish-shaped body move vertically in magnetic conjunction to thereby agitate a medium and supplynutrients to cells.

Description

technical field [0001] The present invention relates to a cell culture device capable of agitating a culture medium, supplying nutrients to cells, oxygenating cells, and culturing a large number of cells with simple operations. Background technique [0002] Pluripotent stem cells (embryonic stem cells, artificial pluripotent stem (iPS) cells) are considered as an important cell source for regenerative medicine because of their unlimited proliferation potential and multi-differentiation potential. Examples of regenerative medicine using stem cells include treatment of liver cirrhosis, blood diseases, and myocardial infarction, construction of blood vessels, regeneration of bones and corneas, and securing of skin for transplantation. In regenerative medicine, stem cells or the like are used to proliferate target cells or internal organs in a petri dish and transplant them into humans. Recently, bone marrow-derived stem cells have been used for angiogenesis, and angina pectori...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/02C12M1/42C12M3/00
CPCC12M27/22C12M25/14C12N5/0667C12N5/0663C12N5/0668C12M1/00C12M1/02C12M1/42C12M3/00C12M27/16
Inventor 千叶俊明李英珍牛房贵树
Owner FULLSTEM CO LTD
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