Cell cDNA chip, preparation method and application thereof

Abstract

The invention discloses a cell cDNA chip, which comprises a substrate and a cDNA template fixed on the substrate. The cDNA template is the cDNA obtained by reverse transcription of the total RNA extracted from a cell strain or primary cell. The cDNA chip also has housekeeping gene primers, which are a pair of forward and reverse primers of a housekeeping gene. The cell cDNA chip provided by the invention can be directly applied to cell strain screening, has short experimental preparation time, only one chip is used each time, the dosage of experimental reagent is saved, the difficulties of mass cell culture and sample extraction by researchers can be effectively solved, the experimental conditions of each sample are consistent, the experimental error is small, and the accuracy and screening efficiency of cell strain screening are improved, therefore the cell cDNA chip can be used for high-throughput screening of cell strains. The invention also provides a preparation method and application of the cell cDNA chip.

Description

technical field [0001] The invention relates to the field of gene chips, in particular to a cell cDNA chip and its preparation method and application. Background technique [0002] Life science and medical research at the level of cell biology is mainly carried out from three aspects: cell structure, cell function, and expression of proteins and various nucleic acids in cells. In order to comprehensively solve scientific research problems and explain various hypotheses and conjectures, the above three aspects complement each other and are indispensable. From an experimental point of view, detecting the expression of various nucleic acids in cells is the most basic part of cell biology research. Therefore, the primary basis for selecting a suitable cell line or primary cell is that the expression level of the target gene or protein should meet the needs of the experiment. [0003] In order to select suitable cell lines or primary cells, the current method that researchers u...

AI Technical Summary

Problems solved by technology

The above method has the following disadvantages: 1) There are many steps and time-consuming; 2) The screening efficiency is extremely low, and high-throughput screening cannot be realized; 3) Each cell line or primary cell to be selected needs to be operated separately according to the above method, and the experimental conditions It is difficult to maintain consistency, and the homogeneity between groups is poor, which affects the accuracy of screening results; 4) Due to the limitations of different cell lines, especially primary cell sources, and experimental funding, researchers usually cannot obtain all the target species at the same time. Cell lines can only be screened within a limited selection range, and the types of cells detected are not complete, which will affect the results of subsequent experiments

[0004] Most of the existing gene chip technology is to fix a large number of DNA fragments (gene probes) of known sequences on the substrate to form a two-dimensional DNA probe array. According to the hybridization sequencing method, a large number of sequences in the sample can be analyzed at one time. Detection and analysis, can be used for gene expression profiling, mutation detection, polymorphism analysis, genomic library mapping and hybridization sequencing, etc., but this method is not suitable for direct application to the screening of various cell lines

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