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Application of miconazole to preparation of tumor resistant medicines

An anti-tumor drug, the technology of miconazole, is applied in the field of medicine to achieve obvious tumor-suppressing effects, stable properties, and the effect of inhibiting proliferation

Inactive Publication Date: 2020-03-27
EAST CHINA NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing anti-tumor drugs are still far from meeting the growing needs of cancer patients, and the current marketed anti-tumor drugs have various adverse reactions. It is a hot spot, and excavating its anti-tumor activity from approved marketed drugs has become a new direction for the development of anti-tumor drugs

Method used

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  • Application of miconazole to preparation of tumor resistant medicines
  • Application of miconazole to preparation of tumor resistant medicines
  • Application of miconazole to preparation of tumor resistant medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1MTS method measures the growth inhibitory effect of miconazole to pancreatic cancer cells

[0056] Pancreatic cancer cells PANC-1 (purchased from ATCC) 3×10 3 Each well was inoculated into a 96-well plate, cultured for 24 hours to allow it to adhere to the wall, and then miconazole (purchased from Shanghai Taosu Biochemical Technology Co., Ltd.) was added to set up 8 concentration gradients, respectively 300 μM, 100 μM, 30 μM, 10 μM, 3 μM, 1 μM, 0.3 μM, 0.1 μM, each concentration set 3 replicate wells. Cells at 37°C, 5% CO 2 After culturing for 72 hours under the same conditions, the culture medium was discarded, and the cell viability was measured with an MTS kit (Promega).

[0057] The test method is as follows: wash pancreatic cancer cell PANC-1 cells with serum-free medium, add pre-prepared MTS chromogenic solution according to the amount of 100 μl / well (add 2ml solution 1 and 100μl solution 2 to 10ml serum-free medium , mix thoroughly). A well witho...

Embodiment 2

[0065] Example 2 The growth inhibitory effect of miconazole on liver cancer cells

[0066] The growth inhibitory effect of miconazole on human liver cancer cells was detected according to the method of Example 1, and the results showed that miconazole had an inhibitory effect on the IC of liver cancer cells SK-HEP1 cells (purchased from the Chinese Academy of Sciences Cell Bank). 50 The value is 13.87μM, the IC of liver cancer cells HepG2 cells (purchased from ATCC) 50 The value is 27.89μM, the IC of liver cancer cells Huh7 cells (purchased from the Chinese Academy of Sciences Cell Bank) 50 The value is 24.50 μM, and the specific results are shown in Table 2.

[0067] Table 2 The IC of miconazole inhibiting the proliferation of liver cancer cells in Example 2 of the present invention 50

[0068]

[0069] The above results show that miconazole has a good inhibitory effect on liver cancer cells SK-HEP1, HepG2, and Huh7.

Embodiment 3

[0070] Example 3 Miconazole inhibits the growth of human lung cancer cells

[0071] The growth inhibitory effect of miconazole on human lung cancer cells was detected according to the method of Example 1, and the results showed that miconazole had an IC on lung cancer cells A549 cells (purchased from ATCC). 50 The value is 19.40μM, the IC of lung cancer cells PC9 cells (purchased from ATCC) 50 The value is 16.83μM, the IC of lung cancer cells H522 cells (purchased from the Chinese Academy of Sciences Cell Bank) 50 The value is 25.90 μM, and the specific results are shown in Table 3.

[0072] Table 3 The IC of miconazole in the embodiment of the present invention 3 inhibiting the proliferation of lung cancer cells 50

[0073]

[0074] The above results show that miconazole has a certain inhibitory effect on lung cancer cells A549, PC9, and H522.

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Abstract

The invention belongs to the field of pharmaceuticals, and discloses an application of miconazole to preparation of tumor resistant medicines. Tumors include pancreatic cancer, liver cancer, lung cancer and stomach cancer. The miconazole is an artificially-synthesized 1-phenylethyl imidazole derivative, and is a broad-spectrum fungus resistant medicine. The miconazole is used as a new tumor resistant medicine or an auxiliary component thereof for development, and is obvious in tumor resistant effects. Therefore, the miconazole is used for resisting tumors, and has great application prospects.

Description

technical field [0001] The invention belongs to the field of medicine and relates to the application of miconazole in the preparation of antitumor drugs. Background technique [0002] Miconazole is a synthetic 1-phenylethyl imidazole derivative, which is a broad-spectrum antifungal drug, which can inhibit and kill Candida, yeast, ascomycete, cryptococcus, dermatophyte and other algae effect. As one of the imidazoles, miconazole acts mainly by interfering with the activity of cytochrome CYP450 to inhibit the biosynthesis of ergosterol, the main sterol in the fungal cell membrane, thereby damaging the fungal cell membrane and Change its permeability and make important intracellular substances leak out. In addition, miconazole can also inhibit the biosynthesis of triacylglycerol and phospholipids in fungi, and inhibit the activity of oxidase and peroxidase, thereby causing the accumulation of hydrogen peroxide in cells, resulting in cell submicrostructural degeneration and ce...

Claims

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Application Information

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IPC IPC(8): A61K31/4174A61P35/00
CPCA61K31/4174A61P35/00
Inventor 卢伟强姜兴武陈思刘明耀
Owner EAST CHINA NORMAL UNIV
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