Bionic simulation synthetic method for photothermal tumor combined treatment nano preparation

A technology of combination therapy and nano-preparation, which is applied in the fields of biochemical equipment and methods, anti-tumor drugs, medical preparations with non-active ingredients, etc., can solve the problems of cumbersome process, pollution, harsh environmental requirements, etc. and film-forming, good biocompatibility, remarkable effect

Active Publication Date: 2020-03-27
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It solves the problem that the traditional synthesis method is too cumbersome, the environment is harsh, and it has certain pollution

Method used

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  • Bionic simulation synthetic method for photothermal tumor combined treatment nano preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Accurately weigh 5 mg of PLGA material, add 20 mL of dichloromethane and dissolve it ultrasonically to obtain a PLGA organic solution with a concentration of 0.25 mg / ml.

[0025] 2) Accurately weigh 5 mg of the AIE material, add 10 mL of dichloromethane and ultrasonically dissolve to obtain an AIE organic solution with a concentration of 0.5 mg / ml.

[0026] 3) The method of synthesizing (SEV+aCD47)@PLGA nanoparticles by thin film hydration method is as follows:

[0027] (1) Place the one-mouth bottle containing 2ml of PLGA solution on the ultrasonic breaker, start ultrasonication, set the time to 10min, the temperature to 0°C, and the ultrasonic frequency to 3s, 1s;

[0028] (2) Add 80uL of AIE solution and 1ml of PVA aqueous solution drop by drop while sonicating, and continue sonicating until completely mixed;

[0029] (3) The product that has been sonicated is immediately added to a round bottom bottle, and is rotated on a rotary evaporator until the dichlorometh...

Embodiment 2

[0032] 1) Accurately weigh 10 mg of PLGA material, add 20 mL of dichloromethane and dissolve it ultrasonically to obtain a PLGA organic solution with a concentration of 0.5 mg / ml.

[0033] 2) Accurately weigh 10 mg of the AIE material, add 10 mL of dichloromethane and ultrasonically dissolve it to obtain an AIE organic solution with a concentration of 1 mg / ml.

[0034] 3) The method of synthesizing (SEV+aCD47)@PLGA nanoparticles by thin film hydration method is as follows:

[0035] (1) Place the one-mouth bottle containing 2ml of PLGA solution on the ultrasonic breaker, start ultrasonication, set the time to 10min, the temperature to 0°C, and the ultrasonic frequency to 3s, 1s;

[0036] (2) Add 80uL of AIE solution and 1ml of PVA aqueous solution drop by drop while sonicating, and continue sonicating until completely mixed;

[0037] (3) The product that has been sonicated is immediately added to a round bottom bottle, and is rotated on a rotary evaporator until the dichlorome...

Embodiment 3

[0040] 1) Accurately weigh 20 mg of PLGA material, add 20 mL of dichloromethane and dissolve it ultrasonically to obtain a PLGA organic solution with a concentration of 1 mg / ml.

[0041] 2) Accurately weigh 20 mg of the AIE material, add 10 mL of dichloromethane and ultrasonically dissolve to obtain an AIE organic solution with a concentration of 2 mg / ml.

[0042] 3) The method of synthesizing (SEV+aCD47)@PLGA nanoparticles by thin film hydration method is as follows:

[0043] (1) Place the one-mouth bottle containing 2ml of PLGA solution on the ultrasonic breaker, start ultrasonication, set the time to 10min, the temperature to 0°C, and the ultrasonic frequency to 3s, 1s;

[0044] (2) Add 80uL of AIE solution and 1ml of PVA aqueous solution drop by drop while sonicating, and continue sonicating until completely mixed;

[0045] (3) The product that has been sonicated is immediately added to a round bottom bottle, and is rotated on a rotary evaporator until the dichloromethane...

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Abstract

The invention discloses a bionic simulation synthetic method for a photothermal tumor combined treatment nano preparation. The method mainly comprises the following steps: 1) preparing a PLGA organicsolution; and 2) synthesizing (SEV+aCD47)@PLGA nanoparticles through a thin film hydration process. Sendai virus (SEV) can activate the body immune function systemically and cause all-around killing and removal of tumors; aCD47 can block signal pathways of CD47 and SIRPa and activate the ability of macrophages to engulf tumor cells; the highly efficient luminescence of aggregation-induced emission(AIE) in an aggregated state realizes visualization of the tumor treatment process; the PLGA has good biocompatibility, no toxicity, good encystations and film-forming properties, and is widely usedin the fields of pharmaceutical, medical engineering materials and modern industry; and the composite nanoparticles having virus immunotherapy can improve the treatment effect of the tumors.

Description

technical field [0001] The present invention relates to a preparation method of a nano-preparation, in particular to a method for biomimetic synthesis of a nano-preparation for combined photothermal tumor therapy by encapsulating Sendai virus (SEV) and CD47 antibody with PLGA. Background technique [0002] At present, tumor is one of the most threatening diseases to human beings in the world. Immunotherapy can treat cancer by activating the human immune system and relying on its own immune function to kill cancer cells and tumor tissues. Therefore, immunotherapy is one of the most effective ways to treat tumors. In view of the fact that the inactivated Sendai virus (SEV) can be used as a foreign body in the body, it can stimulate the systemic immune system to produce a cytokine storm (such as interferon, tumor necrosis factor and various interleukins, etc.), and it has an important role in the anti-tumor treatment process. The active dendritic cells (DC cells, presenting t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61K35/768A61K41/00A61K9/51A61K47/34A61P35/00
CPCA61K39/39558A61K35/768A61K41/0052A61K9/5153A61P35/00C12N2760/18834A61K2300/00Y02A50/30
Inventor 郑斌彭文畅明东刘爽甘霖
Owner TIANJIN UNIV
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