Microfluidic chip and application thereof

Abstract

The present invention relates to the technical field of microfluidics and particularly to a microfluidic chip and an application thereof. A structure design of cap-shaped cavities is matched with control of micro-emulsion droplets (W / O, water in oil), each cap-shaped cavity can provide a completely independent reaction chamber, a heating component 6 (such as a heating coil) is arranged at a far opening end or a near opening end of each cap-shaped cavity for temperature circulation, besides, a chip system can amplify thousands of samples at the same time, then fluorescent signal changes can bedirectly measured in the cap-shaped cavities by fluorescent probes to obtain quantitative results, or the micro-emulsion droplets are pumped out for sequencing, etc. The chip can provide a high-throughput and rapid amplification method and simplify complicated operation processes, so that polymerase chain reaction can be realized in the microfluidic chip.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a microfluidic chip and its application. Background technique [0002] The traditional polymerase chain reaction has complex reagents, primer addition and other operations in the nucleic acid reaction and nucleic acid amplification reaction, and the amplification process requires multiple denaturation, temperature cycles of ligation and extension, and the reaction volume needs to be tens to Hundreds of microliters and completed on a 96-well plate, the system is huge and the operation is complicated are the shortcomings of the traditional polymerase chain reaction. [0003] Microfluidics, also known as Lab-on-a-chip, can integrate the basic functions of traditional biological and chemical laboratories on a tiny chip of a few square centimeters, including sample separation. , preparation, chemical reaction, detection and other operations. [0004] The microfluidic chip has ...

AI Technical Summary

Problems solved by technology

[0002] The traditional polymerase chain reaction has complex reagents, primer addition and other operations in the nucleic acid reaction and nucleic acid amplification reaction, and the amplification process requires multiple denaturation, temperature cycles of ligation and extension, and the reaction volume needs to be tens to Hundreds of microliters and completed on a 96-well plate, the system is huge and the operation is complicated is the disadvantage of the traditional polymerase chain reaction

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