Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A geraniol-producing strain of Hansenula sporogenes and its fermentation method

A technology of Hansenula yeast and grape juice, which is applied in the field of fermentation engineering to achieve the effects of improving flavor and nutritional value, high capacity and increasing geraniol content

Active Publication Date: 2021-03-02
JIANGNAN UNIV +1
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few relevant reports on how to increase the content of geraniol in liquor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A geraniol-producing strain of Hansenula sporogenes and its fermentation method
  • A geraniol-producing strain of Hansenula sporogenes and its fermentation method
  • A geraniol-producing strain of Hansenula sporogenes and its fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the screening and identification of the Hanseniaspora uvarum of high yield geraniol

[0031] Take 10g of highland barley Daqu and dissolve it in 90mL of sterile saline, shake it on a shaker for 30 minutes, and then carry out gradient dilution. The selected dilution is 10 -2 、10 -3 、10 -4 、10 -5 Take 50 μl of each bacterial solution to coat the WL solid plate, and after culturing at 30°C for 48 hours, the colony morphology of the Hanseniaspora uvarum plate is as follows: figure 1 shown.

[0032] Pick a single colony conforming to the phenotypic characteristics of Hanseniaspora uvarum into a 25mL test tube filled with 5mL barley juice medium, and culture at natural pH, 30°C, 200rpm for 36h to become a seed culture solution. The cultivated seed culture liquid was inoculated in a 250 mL Erlenmeyer flask containing 50 mL of highland barley juice medium with a 5% inoculation amount, and fermented for 48 h at natural pH at 30° C. Three strains with higher ge...

Embodiment 2

[0036]Example 2: Comparison of Geraniol produced by Hanseniaspora uvarum A14 and other yeasts

[0037] The bacterial strain that present embodiment comprises has Saccharomyces cerevisiae (Sc), Hyphopichiaburtonii (Hb), Kazachstania unispora (Ku), Wickerhamomyces anomalus (Wa), Torulaspora delbrueckii (Td), Pichia kudriavzevi (Pku), Pichia scaptomyzae (Psc), Pichia membranifaciens (Pme) and Pichia manshurica (Pma), and the strain of the invention Hanseniaspora uvarum A14 (Hu A14). These 10 strains all come from the brewing environment of highland barley wine.

[0038] Pick a single colony of the above 10 strains into a 25mL test tube filled with 5mL barley juice medium, cultivate at natural pH, 30°C, 200rpm for 36h to become a seed culture solution. Inoculate the cultivated seed culture solution with 5% inoculum in a 250mL Erlenmeyer flask containing 50g of sterilized highland barley, at natural pH, 30°C, 200rpm, ferment for 48h, and detect each strain by HS-SPME and GC-MS tec...

Embodiment 3

[0042] Example 3: Detection of Physiological and Biochemical Properties of Hanseniaspora uvarum A14

[0043] Temperature tolerance test: Pick the Hanseniaspora uvarum A14 strain obtained in Example 1, inoculate it in 5ml YPD liquid liquid medium, and culture it at 30°C for 36h to OD 800 In 1.2-1.4. Dilute medium OD with sterile saline 800 To 1, inoculate 0.5mL bacterial liquid into 50ml YPD liquid medium. They were cultured at different temperature gradients of 20°C, 25°C, 30°C, 37°C, 40°C, 42°C and 46°C for 36 hours. The results show that the Hanseniaspora uvarum A14 bacterial strain of the present invention can grow in the temperature range of 20-46 DEG C, and the optimum temperature is 25-35 DEG C (OD 800 Greater than 1).

[0044] Acidity tolerance test: Pick the Hanseniaspora uvarum A14 strain obtained in Example 1, inoculate it in 5ml YPD liquid medium, and culture it at 30°C for 36h to OD 800 In 1.2-1.4. Dilute medium OD with sterile saline 800 To 1, inoculate 0.5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a geraniol-producing grape juice Hansenula sporogenes and a fermentation method thereof, belonging to the technical field of fermentation engineering. The grape juice Hansenia spora uvarum (Hanseniaspora uvarum) A14 CGMCC No.18666 of the present invention has a high ability to produce geraniol, and the concentration of geraniol after 48 hours of fermentation can reach 63.48μg / L, while other dominant yeasts in the liquor fermentation process The highest content of geraniol produced was only 43.59 μg / L; adding the bacterial agent containing Hansenia spora uvarum (Hanseniaspora uvarum) A14 CGMCC No.18666 in the process of brewing liquor can significantly increase the geraniol content in liquor content, and enhance the flavor and nutritional value of liquor.

Description

technical field [0001] The invention relates to a strain of geraniol-producing Hansenula sporogenes and a fermentation method thereof, belonging to the technical field of fermentation engineering. Background technique [0002] Terpenoids are an important component of plant volatiles. As an acyclic monoterpenoid in liquor, geraniol has a mild and sweet smell of roses, and it also has certain health care functions. Applying geraniol to the brewing wine industry and other food fields can significantly improve the flavor of fermented foods and improve the health effects of foods. [0003] Due to the low content of geraniol produced in the fermentation process of liquor, the industry often constructs strains with geraniol production capacity through genetic engineering, such as performing metabolic flow rearrangement in Saccharomyces cerevisiae, or in Escherichia coli, etc. Geraniol synthesis pathways were constructed in model microorganisms with geraniol production capacity to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P7/04C12G3/021C12R1/645
CPCC12N1/16C12P7/04C12G3/021C12N1/145C12R2001/645
Inventor 吴群李善文徐岩冯声宝陈灵娜黄和强
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com