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Test strip, kit and method for determining glycosylated hemoglobin

A technology of test strips and kits, applied in the field of determination of glycosylated hemoglobin and test strips, can solve the problems of expensive fillers, long time, complicated operation, etc., and achieve the effects of short detection time, improved efficiency, and simple operation

Active Publication Date: 2020-04-07
深圳优迪生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the packing material of the current affinity chromatography column method is expensive, the operation is complicated and the time required to obtain the result is long
[0004] Methodological research on HbA1c detection has been carried out for many years, but further improvement is still needed

Method used

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  • Test strip, kit and method for determining glycosylated hemoglobin
  • Test strip, kit and method for determining glycosylated hemoglobin
  • Test strip, kit and method for determining glycosylated hemoglobin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Experimental group 1 Preparation of cation exchange chromatography membranes modified with functional groups

[0081] The cation exchange chromatography membrane prepared with high molecular weight polyethylene as the core raw material has a membrane thickness of 0.6mm and a microchannel pore diameter in the range of 7-15μm. It is modified with hydroxyl groups. The specific modification method is as follows:

[0082] Weigh 0.05 g of EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) (purchased from Sigma) and dissolve it in 100 mL of pure water, then add 500 μL of ethanolamine (purchased from (Sinopharm Group) and 750 μL of concentrated hydrochloric acid (purchased from Sinopharm Group) were stirred evenly, and then 1.5 g of cation exchange chromatography membrane was added, and the reaction was stirred overnight. After the reaction, wash twice with pure water, rinse with 100mM MES buffer, and dry to obtain a cation exchange chromatography membrane modifi...

Embodiment 2

[0093] Embodiment 2 provides a test strip, which can be used as a test strip for detecting glycosylated hemoglobin. Its specific structure is as figure 1 As shown, it includes base plate 1, chromatographic membrane 2, absorbent paper 3, waterproof paper 4, baffle plate 5 and detection hole 6; wherein the base plate is divided into two pieces, and the chromatographic membrane is located on the base plate so that the two base plates are connected, And a detection hole is formed, and the chromatographic membrane passes through the detection hole. At the same time, absorbent paper is set on the bottom plate, one end of the absorbent paper overlaps with one end of the chromatographic membrane, and a baffle is arranged above the chromatographic membrane. The baffle is set corresponding to the detection hole, and the baffle completely blocks the light of the detection hole to avoid Interference from external light. At the same time, cover the waterproof paper on top of the absorben...

Embodiment 3

[0114] Embodiment 3 provides a test strip, which can be used as a test strip for detecting glycosylated hemoglobin. Its specific structure is as figure 2 As shown, it includes: bottom plate 1, first chromatographic membrane 201, second chromatographic membrane 2, absorbent paper 3, waterproof paper 4, first baffle 501, second baffle 502, first detection hole 601 and second detection hole 602 . The bottom plate is divided into three pieces, which are respectively connected by the first chromatographic membrane and the second chromatographic membrane to form the first detection hole and the second detection hole. The first chromatographic membrane passes through the first detection hole, and the second chromatographic membrane passes through the second detection hole. Detection hole. The chromatographic membrane is respectively provided with a first baffle and a second baffle, the first baffle is arranged corresponding to the first detection hole, and is used to block the lig...

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Abstract

The invention provides a test strip, a kit and a method for determining glycosylated hemoglobin. The test strip comprises: a bottom plate, wherein at least one detection hole is formed in the bottom plate; a chromatographic membrane which is located on the bottom plate and passes through the at least one detection hole, wherein at least part of the chromatographic membrane is modified with boric acid groups; absorbent paper positioned on the bottom plate, wherein one end of the absorbent paper is in contact with one end of the chromatographic membrane; a baffle plate arranged on the chromatographic membrane, wherein the baffle plate is arranged corresponding to the at least one detection hole; and waterproof paper which is arranged on the upper surface of the absorbent paper. The kit provided by the invention comprises the test strip. By applying the test strip or the kit, rapid determination of glycosylated hemoglobin can be realized. The determination method has the advantages of theextremely low sample demand, the accurate result, good stability, simplicity in operation, low price and the like, meets the requirements of clinical diagnosis and POCT, is suitable for being used bythe masses, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, and relates to a test strip, a test kit and a method for measuring glycosylated hemoglobin. Background technique [0002] In the 1990s, Western countries such as the United Kingdom and the United States conducted large-scale clinical pathology and physiology research on diabetes. Scientists found that the reduction of diabetic complications was related to the reduction of the percentage of glycosylated hemoglobin (HbA1c) in total hemoglobin (Hb). HbA1c can not only reflect blood sugar control, treatment compliance and other indicators, but also has important value in diabetes prediction, diagnosis, and prognosis judgment. Compared with fasting blood glucose (FPG) and fasting glucose tolerance test (OGTT), HbA1c has several advantages: (1) convenience; (2) better pre-analytical stability; (3) anti-interference performance. HbA1c can accurately reflect the long-term blood sugar control level of p...

Claims

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Application Information

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IPC IPC(8): G01N33/66
CPCG01N33/66
Inventor 艾峰许定
Owner 深圳优迪生物技术有限公司
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