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Fruit extract of eucalyptus globulus labill. and application thereof in medulloblastoma resistance

A medulloblastoma, extract technology, applied in antitumor drugs, medical preparations containing active ingredients, drug combinations, etc. Proliferation, the effect of good application prospects

Inactive Publication Date: 2020-04-10
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still no specific treatment method and suitable drug for medulloblastoma. The main treatment methods include surgery, radiotherapy and chemotherapy, but the five-year survival rate is only about 60%, and most patients suffer long-term treatment side effects. Afflictive, conventional treatments combined with surgery, radiation and chemotherapy often lead to permanent neurocognitive impairment
There is no research report on the inhibition of medulloblastoma proliferation by Eucalyptus eucalyptus fruit and its components

Method used

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  • Fruit extract of eucalyptus globulus labill. and application thereof in medulloblastoma resistance
  • Fruit extract of eucalyptus globulus labill. and application thereof in medulloblastoma resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Extraction and separation of Eucalyptus eucalyptus fruit extract and components thereof

[0031] The dried fruit of Eucalyptus blueis is fully pulverized, soaked in 95% ethanol at room temperature, the extract is filtered out, concentrated and dried to obtain crude ethanol extract of Eucalyptus blueberry fruit. Take the ethanol crude extract of eucalyptus eucalyptus fruit, add deionized water to dissolve it, then add an equal volume of petroleum ether, ethyl acetate, and n-butanol solvents for extraction, and concentrate and dry the extraction solvent to obtain petroleum ether layer extract, acetic acid Ethyl ester layer extract, n-butanol layer extract, water layer extract. Take the ethyl acetate layer extract, adopt 100-200 mesh silica gel to carry out silica gel column chromatography, use successively eluting solvent sherwood oil: ethyl acetate (volume percentage is 100:1, 50:1, 25:1, 10:1 , 5:1, 1:1), pure ethyl acetate, ethyl acetate: ethanol (50:1, 10:1...

Embodiment 2

[0032] Example 2 Establishment of Medulloblastoma Cell Screening Model

[0033] In order to ensure the accuracy and reliability of the activity screening results, the present invention uses two kinds of medulloblastoma cell lines MB39 (mouse medulloblastoma cell line) and Daoy (human medulloblastoma cell line) simultaneously as the active group Sub-screened cell screening models to ensure the accuracy and completeness of activity test data.

Embodiment 3

[0034] Example 3 Ethanol extract of Eucalyptus eucalyptus fruit and its components inhibit the proliferation of medulloblastoma

[0035]In biological activity screening, CCK8 was selected to analyze and detect the effect of alcohol extract and its components (including petroleum ether extract, ethyl acetate extract, n-butanol extract and component Fr.1-40) on medulloblastoma Inhibition of cells. In the presence of electron coupling reagents, CCK8 can be reduced by dehydrogenases in mitochondria to generate highly water-soluble orange-yellow formazan products. The depth of color is directly proportional to cell proliferation and inversely proportional to cytotoxicity. Use a microplate reader to measure the OD value at a wavelength of 450nm, which indirectly reflects the number of viable cells. When the extract to be tested is added to the cell model, the active components can inhibit cell growth or even kill cells, resulting in a reduction in the number of viable cells. By d...

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PUM

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Abstract

The invention, which belongs to the field of traditional Chinese medicines, particularly discloses application of an alcohol extract of eucalyptus globulus labill. fruit and components in preparationof a medicine for preventing and / or treating anti-medulloblastoma. Effective components of fruits of eucalyptus globulus labill. are extracted; and activity screening is performed by utilizing medulloblastoma cells. Experimental results show that the eucalyptus globulus labill. fruit alcohol extract and the components thereof can effectively inhibit the proliferation activity of medulloblastoma and have the broad application prospects in the aspect of preparation and development of drugs for treating tumor diseases.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to the new use and application of Eucalyptus eucalyptus fruit extract and components thereof for inhibiting the proliferative activity of medulloblastoma. Background technique [0002] Medulloblastoma (MB) is a highly invasive tumor among malignant tumors of the central nervous system. It is evolved from primitive neural stem cells. Differentiation potential, belonging to primitive neuroectodermal tumors. Medulloblastoma is the most common childhood malignancy, often occurring in children aged 0 to 8, and occasionally in adults. Although its incidence rate only accounts for 25% of children's tumors, due to its high degree of malignancy, rapid clinical progress, strong invasiveness, and easy spread through the brain and spinal cord, the mortality rate ranks first among children's malignant tumors. At present, there is still no specific treatment method and suitable drug fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/61A61P35/00A61P35/04A61K131/00
CPCA61K36/61A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61P35/00A61P35/04
Inventor 樊慧董素珍游群
Owner EAST CHINA NORMAL UNIV
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