Capsule preservation method for freeze-dried bacteriophage powder

Abstract

The invention discloses a capsule preservation method for freeze-dried bacteriophage powder. The method comprises the following steps: S1, primary treatment of bacteriophage: selecting host bacteria with bacteriophages, inoculating the bacteriophages into a liquid culture medium, carrying out shake culture for about 10 hours, and collecting a supernate containing the bacteriophages; S2, preparation of a bacteriophage protection agent: mixing the bacteriophages and a protective agent together, and fully mixing the bacteriophages and the protective agent together under the stirring of a stirringrod; S3, preparation of freeze-dried powder: placing a mixed solution prepared in the S2 into a freezing chamber for freezing, conducting primary drying under vacuum conditions, adding 10-20 parts ofglycerinum into bacteriophage liquid obtained after primary drying again, then conducting heating, and conducting secondary drying to obtain the freeze-dried bacteriophage powder; and S4, preparationof capsules: loading the freeze-dried powder into capsules in a sterile environment by using a cementing machine, sealing the capsules, and storing the sealed capsules in a shade place. The capsule preservation method of the invention has good preservation effect.

Description

technical field [0001] The invention relates to the technical field of capsule preservation, in particular to a method for preserving bacteriophage freeze-dried powder capsules. Background technique [0002] Phages are viruses that attack bacteria and are also genetic material that confers biological traits on host bacteria. Phages must parasitize in living bacteria and have strict host specificity, which depends on the molecular structure and complementarity of phage adsorption organs and surface receptors of recipient bacteria. Phages are the most prevalent and widespread group of viruses. [0003] The traditional storage method is to make phage into freeze-dried powder, and then store them in a 4-degree freezer or store them in liquid nitrogen, but the preservation effect is poor. For example, the patent publication number is CN201610415919.6, which discloses a phage freeze-dried powder capsule preservation method. The protective agent and the phage liquid are mixed tog...

AI Technical Summary

Problems solved by technology

[0003] The traditional storage method is to make the phage into freeze-dried powder, and then store it in a 4-degree freezer or store it in liquid nitrogen, but the preservation effect is poor.

For example, the patent publication number is CN201610415919.6, which discloses a phage freeze-dried powder capsule preservation method. The protective agent and the phage liquid are mixed together and stored at a temperature of 4 degrees. The preservation measures are still not perfect. , the preservation effect is poor