Capsule preservation method for freeze-dried bacteriophage powder

A preservation method and phage technology, which is applied in the field of phage freeze-dried powder capsule preservation, can solve the problems of insufficient preservation measures and poor preservation effect, and achieve the effect of prolonging the preservation time and good preservation effect

Inactive Publication Date: 2020-04-10
RECOM QINGDAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional storage method is to make the phage into freeze-dried powder, and then store it in a 4-degree freezer or store it in liquid nitrogen, but the preservation effect is poor.
For example, the patent publication number is CN201610415919.6, w

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment discloses a method for storing phage freeze-dried powder capsules, comprising the following steps;

[0025] Preliminary treatment of S1 phage, select the host bacteria with phage, inoculate the phage into the liquid medium, shake and culture for about 10 hours, and collect the supernatant containing the phage;

[0026] S2 To make a phage protective agent, select 15 parts of skimmed milk powder, 1.3 parts of glycerin, 4 parts of glucose, 0.6 parts of calcium chloride, 0.36 parts of sodium sulfate, 1 part of mannitol, 0.1 part of dipotassium hydrogen phosphate, 0.18 parts of sodium dihydrogen phosphate and 0.18 parts of aluminum hydroxide glue, and the phage and the above mixed solution are mixed together, and fully mixed together under the stirring of the stirring bar;

[0027] S3 is made into freeze-dried powder, the mixed solution prepared in S2 is placed in a freezer, frozen, and then dried for the first time under vacuum conditions, and 10 parts are a...

Embodiment 2

[0035] Preliminary treatment of S1 phage, select the host bacteria with phage, inoculate the phage into the liquid medium, shake and culture for about 10 hours, and collect the supernatant containing the phage;

[0036] S2 to make phage protective agent, select 17 parts of skimmed milk powder, 1.6 parts of glycerin, 4 parts of glucose, 1 part of calcium chloride, 0.5 part of sodium sulfate, 1 part of mannitol, 0.03 parts of dipotassium hydrogen phosphate, 0.25 parts of sodium dihydrogen phosphate and 0.06 part of aluminum hydroxide glue, and the phage and the above mixed solution are mixed together, and fully mixed together under the stirring of the stirring bar;

[0037] S3 is made into freeze-dried powder, the mixed solution prepared in S2 is placed in a freezer, frozen, and then dried for the first time under vacuum conditions, and 10- 20 parts of glycerol, then heated, and dried for the second time to obtain phage freeze-dried powder;

[0038] S4 is made into capsules, an...

Embodiment 3

[0041] Preliminary treatment of S1 phage, select the host bacteria with phage, inoculate the phage into the liquid medium, shake and culture for about 10 hours, and collect the supernatant containing the phage;

[0042] S2 To make a phage protective agent, select 15 parts of skimmed milk powder, 1.3 parts of glycerin, 4 parts of glucose, 0.6 parts of calcium chloride, 0.36 parts of sodium sulfate, 1 part of mannitol, 0.1 part of dipotassium hydrogen phosphate, 0.18 parts of sodium dihydrogen phosphate and 0.18 parts of aluminum hydroxide glue, and the phage and the above mixed solution are mixed together, and fully mixed together under the stirring of the stirring bar;

[0043] S3 is made into freeze-dried powder, the mixed solution prepared in S2 is placed in a freezer, frozen, and then dried for the first time under vacuum conditions, and 20 parts are added to the phage liquid after the first drying glycerin, then heated, and dried for the second time to obtain phage freeze-...

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PUM

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Abstract

The invention discloses a capsule preservation method for freeze-dried bacteriophage powder. The method comprises the following steps: S1, primary treatment of bacteriophage: selecting host bacteria with bacteriophages, inoculating the bacteriophages into a liquid culture medium, carrying out shake culture for about 10 hours, and collecting a supernate containing the bacteriophages; S2, preparation of a bacteriophage protection agent: mixing the bacteriophages and a protective agent together, and fully mixing the bacteriophages and the protective agent together under the stirring of a stirringrod; S3, preparation of freeze-dried powder: placing a mixed solution prepared in the S2 into a freezing chamber for freezing, conducting primary drying under vacuum conditions, adding 10-20 parts ofglycerinum into bacteriophage liquid obtained after primary drying again, then conducting heating, and conducting secondary drying to obtain the freeze-dried bacteriophage powder; and S4, preparationof capsules: loading the freeze-dried powder into capsules in a sterile environment by using a cementing machine, sealing the capsules, and storing the sealed capsules in a shade place. The capsule preservation method of the invention has good preservation effect.

Description

technical field [0001] The invention relates to the technical field of capsule preservation, in particular to a method for preserving bacteriophage freeze-dried powder capsules. Background technique [0002] Phages are viruses that attack bacteria and are also genetic material that confers biological traits on host bacteria. Phages must parasitize in living bacteria and have strict host specificity, which depends on the molecular structure and complementarity of phage adsorption organs and surface receptors of recipient bacteria. Phages are the most prevalent and widespread group of viruses. [0003] The traditional storage method is to make phage into freeze-dried powder, and then store them in a 4-degree freezer or store them in liquid nitrogen, but the preservation effect is poor. For example, the patent publication number is CN201610415919.6, which discloses a phage freeze-dried powder capsule preservation method. The protective agent and the phage liquid are mixed tog...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12R1/92
CPCC12N7/00C12N2795/00011
Inventor 李先胜杜新永刘玉庆张庆罗成盛马如霞赵丹丹
Owner RECOM QINGDAO BIOTECH CO LTD
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