Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation of cardiac myosin binding protein C antibody and detection method

A myosin, protein-binding technique with applications in immunology and protein chemistry. It can solve problems such as difficulty in diagnosing AMI, and achieve the effect of high sensitivity and specificity

Pending Publication Date: 2020-04-14
CHANGCHUN HENGXIAO BIOTECH CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The gold standard blood indicator for the diagnosis of acute myocardial infarction (AMI) is troponin T (Cardiac troponin T, cTnT), but the level of cTnT in the blood is very low in the initial stage of AMI, making early diagnosis of AMI difficult

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of cardiac myosin binding protein C antibody and detection method
  • Preparation of cardiac myosin binding protein C antibody and detection method
  • Preparation of cardiac myosin binding protein C antibody and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The invention discloses the process of preparing MYBPC3 specific antibody and establishing a LFIA detection kit for MYBPC3:

[0030] In order to establish a method for detecting MYBPC3, the present invention prepares MYBPC3-C0 recombinant protein antigen for producing MYBPC3-C0 monoclonal antibody or polyclonal antibody, and uses this antibody to establish a method for detecting MYBPC3 and an in vitro diagnostic kit for detecting MYBPC3 by LFIA.

[0031] Specifically, the invention discloses a preparation process of MYBPC3-C0 recombinant protein antigen, the steps are as follows:

[0032] (1) Synthesis of MYBPC3-C0 DNA; (2) Construction of pQE-60-MYBPC3-C0 vector; (3) Expression of MYBPC3-C0-His protein; (4) Separation and purification of MYBPC3-C0 with Ni-NTAAgarose and ion chromatography -His protein; (5) Carry out the process of refolding and concentrating MYBPC3-C0-His protein; (6) Measure the concentration of MYBPC3-C0-His protein, and freeze at -80°C.

[0033] Am...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses preparation of a cardiac myosin binding protein C antibody, and belongs to the technical field of biology. The invention aims to prepare an MYBPC3-C0 antibody, and the MYBPC3-C0 antibody is specifically combined with MYBPC3 and is used for establishing a kit for detecting the MYBPC3 by virtue of lateral flow immunoassay (LFIA). According to the invention, a tag-MYBPC3-C0 recombinant protein vector is constructed, a tag-MYBPC3-C0 protein is expressed by bacteria, yeast, insect cells and animal or human cells, and the tag-MYBPC3-C0 protein is purified into an MYBPC3-C0 antigen. The antibody has the advantages of high sensitivity and strong specificity, can rapidly detect the concentration of MYBPC3 in blood, and is used for early diagnosis of AMI.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to immunology and protein chemistry. Background technique [0002] The gold standard blood index for the diagnosis of acute myocardial infarction (AMI) is troponin T (Cardiac troponin T, cTnT), but the level of cTnT in the blood is very low in the initial stage of AMI, making early diagnosis of AMI difficult. [0003] The ideal marker of AMI is the rapid release from cardiomyocytes into the blood after myocardial infarction, and cardiac myosin-binding protein C (Myosin-Binding Protein C, Cardiac-Type, MYBPC3 or cMyBP-C) is more in line with this standard. [0004] MYBPC3 has 1273 amino acids and a molecular weight of 140KDa structural protein. MYBPC3 belongs to the type III fibronectin superfamily and the immunoglobulin superfamily, and contains three type III fibronectin domains (Fibronectin type 3, FN3) and eight immunoglobulin-like domains (Immunoglobulin, Ig), Pro-Ala structure Domai...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C07K16/06G01N33/68G01N33/558
CPCC07K16/18C07K16/065G01N33/6887G01N33/6893G01N33/558C07K2317/35G01N2800/324G01N2333/47
Inventor 刘斌李宝民关恒王珺楠张金玲刘戈史永丰刘宁李添伟黄景林高敏孙晓婷林琳朱道林孙玉峰王贺
Owner CHANGCHUN HENGXIAO BIOTECH CO LTD