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Mesenchymal stem cell biological scaffold, preparation method and applications thereof

A technology of mesenchymal stem cells and bio-scaffolds, applied in the field of mesenchymal stem cell bio-scaffolds and its preparation, can solve problems such as the lack of effective treatment of arthritis

Inactive Publication Date: 2020-04-21
中冠赛尔生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] To this end, the embodiments of the present invention provide a mesenchymal stem cell bioscaffold and its preparation method and application, so as to solve the problem of lack of effective materials for treating arthritis in the prior art

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  • Mesenchymal stem cell biological scaffold, preparation method and applications thereof
  • Mesenchymal stem cell biological scaffold, preparation method and applications thereof
  • Mesenchymal stem cell biological scaffold, preparation method and applications thereof

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Embodiment 1

[0027] The preparation method of the mesenchymal stem cell bioscaffold of the embodiment of the present invention comprises the following steps:

[0028] 1. Preparation of Chlorinated Chitosan Solution

[0029] Take 0.2 g of chlorinated chitosan and stir in 10 ml of triple distilled water to prepare 2% chlorinated chitosan aqueous solution respectively. Chlorinated chitosan cannot be completely dissolved immediately, and it is suspended in the solution into a floc, which can be placed in a refrigerator at 4°C overnight. What is dissolved in triple distilled water is a milky white clear jelly-like liquid with uniform properties, which is sterilized by high pressure and stored in a 4°C refrigerator for later use.

[0030] 2. Preparation of β-sodium glycerophosphate solution

[0031] Dissolve 4.5g of β-sodium glycerophosphate (GP) in 10ml of triple-distilled water to prepare 45% GP-water solution and 45% GP-DMEM solution. This salt is easy to dissolve. When dissolved in three-...

Embodiment 2

[0038] The preparation method of the mesenchymal stem cell bioscaffold of the embodiment of the present invention comprises the following steps:

[0039] 1. Preparation of Chlorinated Chitosan Solution

[0040] Get chitosan chloride 0.2g and stir in the DMEM medium of 10ml, 2% chitosan chloride DMEM solution. Chlorinated chitosan cannot be completely dissolved immediately, and it is suspended in the solution into a floc, which can be placed in a refrigerator at 4°C overnight. When dissolved in DMEM, it becomes a pink clear viscous liquid, which is sterilized by autoclaving and stored in a 4°C refrigerator for later use.

[0041] 2. Preparation of β-sodium glycerophosphate solution

[0042] Dissolve 4.5g of β-sodium glycerophosphate (GP) in 10ml of DMEM culture medium to prepare a 45% GP-DMEM solution. When dissolved in DMEM, it becomes a clear pink solution. All of them are sterilized by filtration with a filter membrane of 0.22 μm, and placed in 4 ℃ refrigerator for later ...

Embodiment 3

[0049] The preparation method of the mesenchymal stem cell bioscaffold of the embodiment of the present invention comprises the following steps:

[0050] 1. Preparation of Chlorinated Chitosan Solution

[0051] Take 0.2 g of chitosan chloride and stir in 10 ml of triple distilled water to prepare a 2% aqueous solution of chitosan chloride. Chlorinated chitosan cannot be completely dissolved immediately, and it is suspended in the solution into a floc, which can be placed in a refrigerator at 4°C overnight. Dissolved in triple distilled water is a milky white clear jelly-like liquid with uniform properties.

[0052] 2. Preparation of β-sodium glycerophosphate solution

[0053] Dissolve 4.5g of β-sodium glycerophosphate (GP) in 10ml of DMEM culture solution to prepare a 45% GP-DMEM solution. When dissolved in DMEM, it will be a clear pink solution. All of them are sterilized by filtration with a 0.22μm filter membrane and placed in Store in 4°C refrigerator for later use.

...

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Abstract

The embodiment of the invention discloses a mesenchymal stem cell biological scaffold, a preparation method and applications thereof. The method comprises the following steps: dispersing mesenchymal stem cells into a mixture of chlorinated chitosan and beta-sodium glycerophosphate to form the mesenchymal stem cell biological scaffold. According to the embodiment of the invention, chlorinated chitosan and beta-sodium glycerophosphate are used as raw materials to prepare a chitosan hydrogel with temperature sensitivity, ie., the mesenchymal stem cell biological scaffold provided by the embodiment of the invention, wherein the material has the characteristics of stable physical properties, good histocompatibility and good biodegradability, especially has the characteristic of slow solidification at 37 DEG C, is suitable for carrying mesenchymal stem cells to perform osteoarthritis articular cavity injection treatment, plays a role in slow release of cells, and plays a role in long-term treatment.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of biological products, in particular to a mesenchymal stem cell bioscaffold and its preparation method and application. Background technique [0002] Osteoarthritis (OA) mostly occurs in middle-aged and elderly people. It is a painful joint disease characterized by the destruction of articular cartilage. The incidence rate of female patients is higher than that of male patients, especially obese women are more likely to develop the disease, and it is the most common cause of disability. Common bone diseases have a great impact on the life and work of patients. The main treatment methods for arthritis include pain relief, physical therapy, functional exercise, diet conditioning, etc., to relieve clinical symptoms. In the prior art, chitosan or hyaluronic acid is also used alone, but the therapeutic effect is relatively poor. It is urgent to find a material that can be prepared for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/36A61K47/24A61K35/28A61P29/00A61P19/02C12N5/0775
CPCA61K9/06A61K9/0019A61K47/36A61K47/24A61K35/28A61P29/00A61P19/02C12N5/0662C12N2533/72C12N2533/00
Inventor 徐斌江良旗
Owner 中冠赛尔生物科技(北京)有限公司
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