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Method for culturing hydrogen-oxidizing bacteria mixed bacteria by electrolyzed water

A technology of hydrogen oxidation and electrolysis of water, which is applied in the field of microorganisms, can solve problems such as explosions and low solubility of hydrogen gas, and achieve the effects of low energy consumption and avoiding danger

Inactive Publication Date: 2020-04-21
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The hydrogen required for the mixed growth of hydrogen oxidizing bacteria usually comes from gas cylinders, but the solubility of hydrogen is low, and it will cause an explosion if the concentration exceeds the threshold. It is urgent to find a new hydrogen supply method for the mixed growth of hydrogen oxidizing bacteria

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  • Method for culturing hydrogen-oxidizing bacteria mixed bacteria by electrolyzed water
  • Method for culturing hydrogen-oxidizing bacteria mixed bacteria by electrolyzed water

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Embodiment 1

[0027] Construction of the electrolytic water reactor: the electrolytic cell reactor is a 600mL glass bottle with a working volume of 450mL. The cathode is a circular carbon felt with an area of ​​7cm 2 , the anode is a titanium mesh coated with iridium and ruthenium. The distance between the two electrodes is 4 cm. Hydrogen comes from the cathode, oxygen comes from the anode, and air (inlet flow rate is 150mL / min). The reactor was stirred with a magnetic stirrer at a speed of 120 rpm, the temperature was controlled at 20 ± 0.5 °C with a water bath, and the pH was set to 7.0 ± 0.2 by 1 m NaOH and 1 m HCl.

[0028] Using the above electrolytic cell reactor, set different current densities: 1.43mA / cm 2 ,2.14mA / cm 2 ,2.86mA / cm 2 , and 4.29mA / cm 2 , Determine the amount of hydrogen produced by gas chromatography to ensure the smooth progress of hydrogen production by electrolysis of water.

[0029] Add the pre-enriched hydrogen oxidizing bacteria mixture to the electrolytic...

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Abstract

The invention provides a method for culturing hydrogen-oxidizing bacteria mixed bacteria by electrolyzing water, and the method comprises the following steps of: S1, filling a culture medium into an electrolytic cell, inserting an anode and a cathode of an electrolysis device, electrifying the electrolysis device, and introducing air into the culture medium; and S2, inoculating pre-enriched hydrogen-oxidizing bacteria mixed bacteria into the culture medium for culture, and hydrogen generated by water in the electrolytic culture medium can be directly used as a hydrogen source for the growth ofthe hydrogen-oxidizing bacteria mixed bacteria, so that the danger caused by using a gas cylinder is avoided, and the method has the advantages of low energy consumption and environmental friendliness.

Description

technical field [0001] The invention relates to a method for electrolyzing water to cultivate mixed bacteria of hydrogen oxidizing bacteria, which belongs to the technical field of microbes. Background technique [0002] Hydrogen oxidizing bacteria (HOB) are a class of bacteria that can utilize hydrogen, oxygen and carbon dioxide. HOB's value-added products include polyhydroxyalkanoates (PHA), proteins or plant growth promoters. The nitrogen source of HOB is ammonia or nitrate, which is different from ammonia oxidizing bacteria and hydrogenophilic denitrifying bacteria. Studies have reported that Cupriavidus eutrophus B-10646 (HOB pure bacteria) can synthesize polyhydroxyalkanoate (PHA) with a dry cell weight of 85%. The promotion effect of Burkholderia spp (HOB pure culture) on root elongation of spring wheat seedlings increased from 57% to 254%. However, there are few studies on HOB mixed cultures, and it was recently reported that HOB mixed cultures can produce protein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C25B1/04
CPCC12N1/20C25B1/04Y02E60/36
Inventor 曾建雄张伟张放周顺桂
Owner FUJIAN AGRI & FORESTRY UNIV