Microbial preparation with formaldehyde removal effect as well as preparation method and application thereof
A microbial preparation and formaldehyde technology, applied in the field of microbial preparations, can solve the problems of nail softening, poor effect, easy fatigue, etc., and achieve the effect of good removal effect, wide energy source, and strong adaptability
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Embodiment 1
[0038] Embodiment 1 Microbial preparation with formaldehyde removal function of the present invention and preparation method thereof
[0039] s1), each functional bacteria were obtained through pure culture
[0040] Halomonas, arsenic-oxidizing bacteria and Lactobacillus were activated according to conventional operations, and cultured in the culture medium, adjusted to pH 7.3, and cultured at 37°C for 24 hours; formaldehyde was added to the culture medium to reach a final mass concentration of 0.2% , and continue to cultivate for 36 hours, and the surviving colonies after the end of the cultivation are set aside.
[0041] The medium of the Halomonas is: LB liquid medium containing 3% NaCl by mass fraction; the medium of the arsenic-oxidizing bacteria is the LB medium; the medium of the Lactobacillus is the MRS medium.
[0042] s2), each functional bacterium obtained in step s1) is cultivated respectively
[0043] The clean Halomonas, arsenic-oxidizing bacteria and Lactobaci...
Embodiment 2
[0047] Example 2 The microbial preparation with formaldehyde removal function of the present invention and its preparation method
[0048] s1), each functional bacteria were obtained through pure culture
[0049] Halomonas, arsenic-oxidizing bacteria and Lactobacillus were respectively activated according to routine operations, and cultured in the culture medium, the pH was adjusted to 7.2, and cultured at 37°C for 30 hours.
[0050] The medium of the Halomonas is: LB liquid medium containing 8% NaCl by mass fraction; the medium of the arsenic-oxidizing bacteria is MSM medium; the medium of the Lactobacillus is LB medium.
[0051] s2), each functional bacterium obtained in step s1) is cultivated respectively
[0052] The clean Halomonas, arsenic-oxidizing bacteria and Lactobacillus in step s1) are picked out and inoculated in the culture medium respectively; the culture medium of each functional bacteria is the same as that of s1.
[0053] s3), mixed culture
[0054] The ha...
Embodiment 3
[0056] Embodiment 3 Microbial preparation with formaldehyde removal function of the present invention and preparation method thereof
[0057] s1), each functional bacteria were obtained through pure culture
[0058] Halomonas, arsenic-oxidizing bacteria and Lactobacillus were activated according to conventional operations, and cultured in the culture medium, the pH was adjusted to 7.4, and cultured at 37°C for 36 hours; formaldehyde was added to the culture medium to a final concentration of 0.5%. The culture was continued for 30 hours, and the surviving colonies were used after the culture was over.
[0059] The medium of the Halomonas is: LB liquid medium containing 12% NaCl by mass fraction; the medium of the arsenic-oxidizing bacteria is beef extract peptone medium; the medium of the Lactobacillus is RSMA medium .
[0060] s2), each functional bacterium obtained in step s1) is cultivated respectively
[0061] The Halomonas, Arsenic Oxidizing Bacteria and Lactobacillus t...
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