A microbial self-healing method for tunnel lining concrete cracks
A technology for microbial restoration and concrete, which is applied in the interdisciplinary field of microbiology and civil engineering materials, can solve the problems of reduced number of microorganisms surviving, loss of self-repairing effect, etc., and achieves the effects of delaying the deterioration process, convenient operation, and prolonging the effective survival period
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Embodiment 1
[0045] A preparation method of microbial remediation agent, its specific steps are as follows:
[0046] (1) After activating Bacillus colii, inoculate it into a liquid medium, then cultivate it for 32 hours at a temperature of 35° C. to obtain a bacterial liquid, and then centrifuge the obtained bacterial liquid with a centrifuge at a speed of 4000 r / min for 30 min to obtain Bacterial slime, the composition of the above liquid medium is: ultrapure water 1L, yeast powder 5g, trimethylglycine 4.5g, tryptone 10g, ammonium sulfate 5g, glutamic acid 2g, urea 10g;
[0047] (2) Dilute the sludge with sterilized distilled water to a concentration of 5.5×10 7 cells / mL, make a bacterial suspension, and add trioctyl phosphate and triallylamine with a content of 1wt‰ and 1wt‰ respectively, crush the carrier coral reef calcareous sand to a particle size of 2mm, and then put it into the bacterial suspension , put the bacterial suspension under the pressure of -0.07MPa and soak for 32 hours...
Embodiment 2
[0051] A microbial self-repair method for tunnel lining concrete cracks, the specific steps are as follows:
[0052] (1) Put cement, natural stone, sand, silica fume, calcium chloride, polypropylene fiber, water reducing agent and water into the mixer, and stir evenly;
[0053] (2) Then put the microbial restoration agent prepared in Example 1 into it, and then stir for 2 minutes, put the mixed concrete into the mould, and perform natural maintenance to obtain the tunnel lining concrete with the function of microbial self-repairing cracks. The weight ratio of the agent in the concrete is 13%.
Embodiment 3
[0055] A preparation method of a microbial remediation agent, the only difference between this embodiment and Example 1 is that the protective layer slurry used in step (4) also contains 0.05wt% phenylacetic acid-2-methylpropyl ester and 0.08wt% % of styrene oxide, after the two are adsorbed on the carrier, due to its hydrophobicity, the intermolecular arrangement of the protective layer material changes, and the hydrophobic group is arranged on the outermost side, which makes the hydrophilicity of the carrier significantly reduced and the hydrophobicity increased, thereby avoiding Bacteria or spores are revived in advance when they encounter water in concrete, and the dormant period is not affected by water seepage. In addition, they can also participate in the deposition of calcium carbonate after the carrier is broken, enhancing the impermeability of the cracks, and thus making the repaired concrete more resistant to freezing and thawing. The loss of compressive strength is ...
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