Nano-probe for diagnosis and treatment based on copper phthalocyanine molecule and its preparation and application

A nanoprobe, copper phthalocyanine technology, applied in the field of biomedicine, can solve the problems of detection and treatment limitations, poor solubility, low molecular selectivity, etc., achieve good singlet oxygen generation ability, simple synthesis, and simplified complexity Effect

Active Publication Date: 2022-06-21
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional photosensitive molecules have defects such as poor solubility and low molecular selectivity, which limit their use as detection and treatment methods

Method used

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  • Nano-probe for diagnosis and treatment based on copper phthalocyanine molecule and its preparation and application
  • Nano-probe for diagnosis and treatment based on copper phthalocyanine molecule and its preparation and application
  • Nano-probe for diagnosis and treatment based on copper phthalocyanine molecule and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1 Preparation of integrated probe for diagnosis and treatment based on copper phthalocyanine molecules

[0084] (1) Disperse 1 g of original hexagonal boron nitride powder in a certain amount of concentrated sulfuric acid under magnetic stirring. 0.5g potassium permanganate (KMnO 4 ) was slowly added to the mixed system, and the reaction was continuously stirred for 12 hours. Then 10 mL of analytically pure hydrogen peroxide (H2O) was added to the reaction system 2 O 2 ). The resulting suspension was sonicated for 1 hour and then centrifuged at 10,000 rpm for 10 minutes to remove large h-BN powder. The supernatant liquid was washed with ultrapure water until the pH of the filtrate was neutral. Finally, the product was placed in a vacuum oven and dried at 60 °C for 24 h to obtain hexagonal boron nitride nanosheets (h-BNNS).

[0085] The particle size range of the hexagonal boron nitride nanosheet (h-BNNS) is 80-100 nm, and the thickness is about 15 nm. fig...

Embodiment 2

[0089] The nanoprobes prepared in Example 1 were used for the diagnosis and treatment of tumors, and the specific methods were as follows:

[0090] (1) The nanoprobes (CuPc@HG@BN) were dispersed in phosphate buffer so that the final concentration of the probes was 100 μg / ml. The probes were then reacted with 0.1 fM-20 pM microRNA-21 for 1 h, and then the Raman intensity of the solution was measured at an excitation wavelength of 780 nm. figure 2 is the Raman spectrum and calibration curve of the nanoprobe (CuPc@HG@BN) responding to different concentrations of microRNA-21, from figure 2 As can be seen in a, as the concentration of microRNA-21 increases, the characteristic peak of CuPc molecule is 1530 cm -1 The Raman intensity gradually decreases at , and the characteristic peak of h-BNNS is 1367 cm -1 The Raman intensity at , remains unchanged by I 1530 / I 1367 The linear relationship with the logarithm of the microRNA-21 concentration yields a detection limit of 0.06 fM...

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Abstract

The invention relates to a nano-probe based on copper phthalocyanine molecules integrating detection, imaging and treatment. The nano-probe includes hexagonal boron nitride nanosheets, copper phthalocyanine molecules and two groups of oligonucleotide binding sequences. One end of the two sets of oligonucleotide sequences designed by the present invention is a base pair for detecting and cyclically amplifying microRNA-21, and the other end is an AS1411 sequence. The AS1411 sequence can not only recognize the highly expressed nucleolin on the surface of tumor cells for targeting, but also carry poorly water-soluble copper phthalocyanine molecules based on the G-quadruple formed under specific conditions. Then, the nucleotide sequence loaded with copper phthalocyanine is adsorbed on the surface of the hexagonal boron nitride nanosheet through the π-π stacking effect and enters the tumor cells. The invention also discloses the application of the above-mentioned integrated nanometer probe for diagnosis and treatment in the diagnosis and treatment of tumor cells and living bodies. The integrated nanoprobe for diagnosis and treatment of the present invention can perform in-situ Raman diagnosis on tumor cells, has high sensitivity and strong specificity, and can realize photodynamic therapy at the same time, and has certain application value in biomedicine and clinical diagnosis and treatment.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an integrated nano-probe for diagnosis and treatment based on copper phthalocyanine molecules and its application. Background technique [0002] The integration of diagnosis and treatment is an emerging intelligent method in the medical field. It is a collaborative system with both diagnosis and treatment capabilities. It has become a highly concerned keyword in cancer research. Ideally, smart and effective diagnostic-integrated probes should meet the following criteria: (i) surveillance and treatment modalities are specifically activated at the disease site to avoid background interference and side effects; in particular, activation of probes with endogenous cancer markers Needles are ideal to provide precise control; (ii) probes should have high detection sensitivity and therapeutic efficacy; (iii) monitoring and treatment modalities need to be correlated in each probe to provide hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/54A61K47/69A61K49/00A61P35/00B82Y5/00B82Y20/00
CPCA61K41/0076A61K47/6923A61K47/549A61K49/0013A61K49/0093A61P35/00B82Y20/00B82Y5/00
Inventor 田阳刘嘉郑婷婷
Owner EAST CHINA NORMAL UNIV
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