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A gene apx1 that enhances plant resistance to lepidopteran pests and its application

A lepidopteran and gene technology, applied in the field of genetic engineering, can solve problems such as weakening the effect of pest control, and achieve the effect of significant repelling effect, increased enzyme activity, and increased larval mortality

Active Publication Date: 2020-11-20
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As we all know, BT protein is a kind of biopesticide, which can effectively kill lepidopteran pests such as diamondback moth, and has been widely used. However, many studies have found that various lepidopteran pests have developed resistance to BT, which has weakened the resistance to insect pests. Therefore, it is urgent to find new ways to resist insects

Method used

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  • A gene apx1 that enhances plant resistance to lepidopteran pests and its application
  • A gene apx1 that enhances plant resistance to lepidopteran pests and its application
  • A gene apx1 that enhances plant resistance to lepidopteran pests and its application

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Embodiment 1

[0023] 1. Materials

[0024] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0025] 2. Method

[0026] 2.1 Cloning of Arabidopsis APX1 gene

[0027] 2.1.1 The wild-type Col-0 variety of Arabidopsis thaliana was used as a material to extract RNA, and the extracted total RNA was reverse-transcribed to synthesize the first strand of cDNA, which was used as a template for PCR amplification.

[0028] 2.1.2 Use designed specific primers:

[0029] APX1-F: (5'>GGTTGAAGGATTTTG<3')

[0030] APX1-R: (5'>ATTGTGGGAGGATGGTGCC<3')

[0031] Carry out amplification, obtain the gene fragment of 666bp (electrophoresis result such as figure 1 shown), connected to the T cloning vector PEASY-T5 simple vector, obtained T5-APX1 transformed into Escherichia coli, picked positive clones and sequenced, the sequencing ...

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Abstract

The invention discloses a gene APX1 for enhancing lepidoptera pest resistance of plants and application of the gene APX1. The gene has a nucleotide sequence as shown in SEQ ID NO.1 or a gene sequencewith high homology as the gene, and an amino acid sequence coded by the gene is as shown in SEQ ID NO.2. The gene belongs to an RNA 5'cap binding protein gene. A gene mutant can effectively inhibit the growth and development of plutella xylostella and play a poisoning role. Besides, arabidopsis thaliana is used as a model plant, and the insect-resistant gene has good colinearity with homologous genes of other crops and has high research and application values. The invention provides important gene resources for developing natural insect-resistant strains for crops such as oilseed rape and corn.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a gene APX1 for enhancing plant resistance to lepidopteran pests and its application. Background technique [0002] In agricultural production, pests are one of the important reasons affecting crop yield and quality, and cause huge economic losses to farmers every year. Lepidoptera includes two classes of insects, moths and butterflies, belonging to the subclass Pteroptera and holometabolism. This order is the second largest order in the class Insecta after Coleoptera. The distribution range is extremely wide, and the tropical species is the most abundant. Most types of larvae damage all kinds of cultivated plants, and the larger ones often eat up the leaves or drill into the branches. Smaller ones tend to curl leaves, adorn leaves, form sheaths, spin silk and form nets, or drill into plant tissues to feed and cause damage. Adults usually use nectar as supplementa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415A01P7/04
CPCC07K14/415
Inventor 李培金陈红亿陶珍王传宏陈晨
Owner ANHUI AGRICULTURAL UNIVERSITY
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