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Decellularization method for tissue engineering scaffold

A decellularization and cell technology, applied in the field of decellularization of biological materials, can solve the problems of complicated processing, damage, unsatisfactory mechanical strength or biological activity, etc.

Active Publication Date: 2020-05-01
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Repeatedly, the process is very complicated
And the operation of taking out the biological material multiple times is likely to cause microbial contamination and damage to the biological material
And the shaking table requires uninterrupted driving strength, so the energy consumption is also relatively large
[0007] In addition, the current method mainly uses physical, chemical and biological factors to directly cause active intact cells to lyse, and then wash to remove cell debris
However, from the perspective of clinical application effect, the mechanical strength or biological activity of the product is still unsatisfactory

Method used

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  • Decellularization method for tissue engineering scaffold
  • Decellularization method for tissue engineering scaffold
  • Decellularization method for tissue engineering scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0127] like figure 1 As shown, the decellularization device includes a first pipeline 200, a first container 3, a second pipeline 201, a second container 5, a third pipeline 202, and a third container 8 connected to the third pipeline 202, which are sequentially sealed and communicated; One end of the first pipeline 200 is connected to the first opening 300 of the first container 3; one end of the second pipeline 201 is connected to the second opening 301 of the first container 3; the other end of the second pipeline 201 is connected to the third pipeline One end of 202 is respectively connected to the two ends of the second container 5; the second container 5 is used to place the biological material 6, and the biological material 6 cannot enter the second pipeline 201 and the third pipeline 202, and the solution includes cells It can enter the second pipeline 201 and the third pipeline 202 . The position of the third container 8 is higher than that of the second container 5,...

Embodiment 2

[0129] Another embodiment of the present invention, such as figure 2 As shown, the decellularization device also includes a negative pressure suction device 1, the negative pressure suction device 1 can be a circulating water vacuum pump with a power switch 100 and a vacuum port 101 on it. There are three openings on the first container 3 , which are the first opening 300 and the second opening 301 , wherein the first opening 300 is connected to the vacuum interface 101 on the negative pressure suction device 1 through the first pipeline 200 . The decellularization device may also include a glass tube 4 , one end of the glass tube 4 is inserted into the first container 3 through the second opening 301 on the first container 3 ; the other end is connected to one end of the second pipeline 201 . In this embodiment, the second opening 301 on the first container 3 can be plugged with a rubber stopper, and the glass tube 4 can be inserted into the third container 3 through the rub...

Embodiment 3

[0138] In one of the embodiments of the present invention, in embodiment 1 figure 2 On the basis of the decellularization device, the decellularization liquid (used decellularization liquid) in the first container 3 can be recycled. like Figure 4 As shown, there is used decellularization liquid (waste liquid) in the first container 3, and one end of the glass tube 4 is inserted below the liquid level of the waste liquid (wherein the first container 3 is ventilated, for example, the rubber stopper can be removed).

[0139] The third container 8 is replaced by a fourth container 11 . The fourth container 11 has an opening 110 , an opening 111 and an opening 112 , and the opening 110 is connected to the vacuum interface 101 on the negative pressure suction device 1 through the first pipeline 200 .

[0140] When the decellularization device starts to work, the first opening 300 and the third opening 302 on the first container 3 are closed. When the negative pressure suction d...

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Abstract

The invention belongs to the technical field of tissue engineering, and particularly relates to a decellularization method. The decellularization method comprises the following steps: placing a to-be-treated biological material into a container, and allowing a cell eluent to flow through the container until decellularization of the biological material is finished. By adoption of the decellularization method for decellularization of the biological material, the biological material can be thoroughly treated; the decellularization effect is greatly improved; and the internal structure and mechanical properties of the biological material are well retained. The method provided by the invention can automatically discard a waste liquid and realize automatic cleaning, and greatly reduces the operation steps and water consumption in the cell elution and cleaning processes.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, in particular to a method for decellularizing biological materials. Background technique [0002] ECM (Extracellular Matrixc, extracellular matrix) is the main tissue component of various biological materials. It provides a suitable place for the survival and activity of cells, participates in the regulation of embryonic development, determines the adhesion and migration of cells, and plays an important role in wound repair and fibrosis. , cell growth, differentiation, metabolism and tumorigenesis and metastasis play an important role. Therefore, ECM is widely used to make medical implant materials to actively induce and promote the migration, adhesion, proliferation and differentiation of surrounding cells. Biomaterials contain cellular components in addition to ECM, and cellular components of xenogeneic biomaterials are prone to elicit immune responses. [0003] Therefore, when ECM...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3687A61L27/3691A61L27/3612A61L27/50A61L2430/40
Inventor 吕红斌史强胡建中陈灿赵春风
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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