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Method for inhibiting methane production by anaerobic methanogenesis mixed bacteria

An anaerobic methane production and methane production technology, applied in the field of microorganisms, can solve the problems of high cost and restriction of industrial application, and achieve the effect of long-lasting inhibition, low cost and good inhibition effect

Pending Publication Date: 2020-05-01
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are several microorganisms that can consume BES in mixed-bacteria anaerobic fermentation, and if there is not enough BES, the methanogenic mixed bacteria will be reactivated
In addition, the high cost of BES also restricts its industrial application

Method used

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  • Method for inhibiting methane production by anaerobic methanogenesis mixed bacteria
  • Method for inhibiting methane production by anaerobic methanogenesis mixed bacteria
  • Method for inhibiting methane production by anaerobic methanogenesis mixed bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Sludge from a medium-temperature methanogenic anaerobic reactor of Shandong Huayi Corn Technology Co., Ltd. was passed through a 200-mesh iron sieve to remove non-biological impurities. Use the anaerobic inorganic salt medium to suspend the above sludge, vortex and oscillate to mix evenly, place the centrifuge at 8000rpm, centrifuge for 5 minutes, and remove the supernatant; repeat the above steps 3 times to ensure that organic matter and other interference factors are removed.

[0028] Inoculate the cleaned sludge into a 120mL serum bottle and add 20mL medium at the same time to ensure that the amount of microorganisms added is around 1-2g / L. Nitrogen gas was aerated for 15 minutes, and the above serum bottle was sealed with a butyl rubber stopper and an aluminum cap. Use a vacuum pump to remove the air from the top of the serum bottle so that the air pressure in the headspace is -0.1Mpa. 100 mL (hydrogen / carbon dioxide = 80% / 20%) mixed gas was introduced. The amount...

Embodiment 2

[0034] Using the same conditions as in Example 1, changing the addition of sodium chloride to free acetic acid as an inhibitor, the concentration of free acetic acid in the medium is 0g / L, 001g / L, 0.03g / L, 0.05g / L, 0.08 g / L,0.09g / L,0.16g / L,0.18g / L,0.27g / L,0.46g / L,0.54g / L,0.77g / L,0.81g / L,1.09g / L,1.53 g / L, 1.82g / L, 2.3g / L, 3.64g / L, to detect the production of methane, the experimental results are as follows figure 2 shown.

[0035] Depend on figure 2 It can be seen that as the concentration of free acetic acid increases, methane is produced less, and the concentration of free acetic acid that produces a 50% methane inhibition rate is 0.31 g / L.

Embodiment 3

[0037] Using the same conditions as in Example 1, changing the addition of sodium chloride to add mass percentage is 0%, 2%, 4%, 6%, 8%, and the coupling concentration of sodium chloride of 10% is 0g / L, 001g / L,0.03g / L,0.05g / L,0.08g / L,0.09g / L,0.16g / L,0.18g / L,0.27g / L,0.46g / L,0.54g / L,0.77g / L L, 0.81g / L, 1.09g / L, 1.53g / L, 1.82g / L, 2.3g / L, 3.64g / L free acetic acid is used as an inhibitor to detect the production of methane. The experimental results are as follows image 3 shown.

[0038] Depend on image 3 It can be seen that sodium chloride coupled with free acetic acid can inhibit the production of methane, and 0.14g / L free acetic acid+2% sodium chloride or 0.12g / L free acetic acid+4% sodium chloride can achieve a 50% methane inhibition rate. The concentration of sodium chloride and free acetic acid used is lower than that of sodium chloride used alone, and is also lower than that of free acetic acid used alone, indicating that sodium chloride coupled with free acetic acid inh...

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Abstract

The invention provides a method for inhibiting methanogenesis of anaerobic methanogenesis mixed bacteria, wherein sodium chloride and acetic acid are added into the culture medium, so that the methanogenesis inhibiting effect is good, and the inhibiting effect is lasting.

Description

technical field [0001] The invention relates to a method for inhibiting methane production by anaerobic methanogenic mixed bacteria, which belongs to the technical field of microorganisms. Background technique [0002] The degradation process of organic waste in mixed-bacteria anaerobic fermentation can be divided into four stages, namely hydrolysis, acidification, acetogenic and methanogenic. After methanogenesis was inhibited, volatile fatty acids (VFAs) such as acetate, propionate, and butyrate accumulated significantly during the metabolism of acidogens. Important chemical product precursors of VFAs. Conventional methods of inhibiting methanogenic activity include heat treatment and non-specific / specific inhibitors. After heat treatment, non-spore-forming microorganisms are killed, while some spore-forming bacteria such as Clostridiaceae and Thermoanaerobes may survive. However, heat treatment can only temporarily inhibit the methanogenic mixed bacteria. Furthermore,...

Claims

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Application Information

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IPC IPC(8): C02F11/04C10L3/08
CPCC02F11/04C10L3/08Y02E50/30
Inventor 曾建雄张伟张放
Owner FUJIAN AGRI & FORESTRY UNIV
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