Method for inhibiting methane production by anaerobic methanogenesis mixed bacteria
An anaerobic methane production and methane production technology, applied in the field of microorganisms, can solve the problems of high cost and restriction of industrial application, and achieve the effect of long-lasting inhibition, low cost and good inhibition effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0026] Example 1
[0027] The sludge will be taken from the medium-temperature methane-producing anaerobic reactor of Shandong Huayi Corn Technology Co., Ltd., and the sludge will be passed through a 200-mesh iron sieve to remove non-biological impurities. Suspend the above-mentioned sludge in an anaerobic inorganic salt medium, vortex and mix evenly, place a centrifuge at 8000 rpm, centrifuge for 5 minutes, and remove the supernatant; repeat the above steps three times to ensure that organic matter and other interference factors are removed.
[0028] Inoculate the cleaned sludge into a 120mL serum bottle and add 20mL medium to ensure that the amount of microorganisms added is about 1-2g / L. Aerate with nitrogen for 15 minutes, and seal the serum bottle with butyl rubber stopper and aluminum cap. Use a vacuum pump to draw the air off the top of the serum bottle so that the air pressure in the headspace is -0.1Mpa. Pass in 100mL (hydrogen / carbon dioxide=80% / 20%) mixed gas. The amo...
Example Embodiment
[0033] Example 2
[0034] Using the same conditions as in Example 1, the addition of sodium chloride was changed to free acetic acid as an inhibitor. The concentration of free acetic acid in the medium was 0g / L, 001g / L, 0.03g / L, 0.05g / L, 0.08 g / L, 0.09g / L, 0.16g / L, 0.18g / L, 0.27g / L, 0.46g / L, 0.54g / L, 0.77g / L, 0.81g / L, 1.09g / L, 1.53 g / L, 1.82g / L, 2.3g / L, 3.64g / L, detect methane production, the experimental results are as follows figure 2 Shown.
[0035] by figure 2 It can be seen that as the concentration of free acetic acid increases, the production of methane decreases, and the concentration of free acetic acid that produces a 50% methane inhibition rate is 0.31 g / L.
Example Embodiment
[0036] Example 3
[0037] Using the same conditions as in Example 1, the addition of sodium chloride was changed to adding mass percentages of 0%, 2%, 4%, 6%, 8%, and 10%. The coupling concentration of sodium chloride was 0g / L, 001g / L, 0.03g / L, 0.05g / L, 0.08g / L, 0.09g / L, 0.16g / L, 0.18g / L, 0.27g / L, 0.46g / L, 0.54g / L, 0.77g / L, 0.81g / L, 1.09g / L, 1.53g / L, 1.82g / L, 2.3g / L, 3.64g / L free acetic acid as an inhibitor to detect methane production, the experimental results are as follows image 3 Shown.
[0038] by image 3 It can be seen that sodium chloride coupled with free acetic acid can inhibit the production of methane. 0.14g / L of free acetic acid + 2% sodium chloride or 0.12g / L of free acetic acid + 4% sodium chloride can achieve a 50% methane inhibition rate. The concentration of sodium chloride and free acetic acid used is lower than that of sodium chloride used alone, and at the same time lower than the concentration of free acetic acid used alone, indicating that sodium chlori...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap