Fermentation preparation method of eurotium cristatum melanin

A coronoid saccharomyces and melanin technology, applied in biochemical equipment and methods, fermentation, chemical instruments and methods, etc., can solve the problems of expensive tyrosinase, unfavorable factory production, complex synthesis process, etc., and achieve Shorten the extraction time, increase the yield, the effect of high yield

Pending Publication Date: 2020-05-01
MINNAN NORMAL UNIV
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Problems solved by technology

Traditional melanin synthesis mainly uses enzymatic and chemical methods, but due to the expensive raw materials and tyrosinase, the synthesis process is complicated and the cost is high, which is not conducive to large-scale industrial production
Microbial-derived melanin has been gradually discovered by scholars because of its easy culture and high yield. Many scholars use bacteria, molds or fungi to produce melanin. Considering the safety of strains, most strains (such as: Escherichia coli, thuringiensis Bacillus, etc.) There are still some obstacles in the application of melanin produced in food

Method used

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  • Fermentation preparation method of eurotium cristatum melanin

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Embodiment 1

[0015] Put the coronoid loose capsule strain into sterilized 400 ml liquid medium (1L culture bottle), the formula of the liquid medium is: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, potassium chloride 0.5g, ferrous sulfate 0.01g, sucrose 30g, distilled water 1L, pH 5.5, inoculum size 1%. Put it into a full-temperature shaker for cultivation, the cultivation condition is 28°C, 180r / min, when the cultivation reaches 5 days, a large number of hyphae balls of S. coronis will be produced in the culture bottle. Place the silk balls in a 4°C refrigerator and let them stand for 8-10 hours, then continue to put them in a full-temperature shaker at 28°C, 180r / min for cultivation, and a large amount of melanin molecules will be observed to be secreted into the medium the next day. The base began to turn dark brown, and when the culture reached the 10th day, the culture was terminated, and the melanin was ready to be harvested.

[0016] After ...

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Abstract

The invention provides a fermentation preparation method of eurotium cristatum melanin which comprises the following steps: removing mycelia from fermentation liquor of eurotium cristatum strain whichis separated from Fuzhuan tea and has high melanin yield, adding 3mol / L hydrochloric acid to regulate the pH value to 3, standing at low temperature for 4 hours, naturally precipitating melanin molecules, centrifuging, and collecting melanin remained in the precipitate, dissolving melanin with 0.01 mol / L NaOH solution, adding 3 mol / L hydrochloric acid again to enable the pH value to be 3, precipitating melanin molecules, purifying the melanin molecules through precipitation and re-dissolution processes for 3-5 times, washing the final precipitate with ultrapure water for 3-5 times, and thoroughly drying the melanin molecules through freeze-drying. Melanin molecules are proved to be eumelanin through structural characterization, and further animal experiments show that the melanin of the eurotium cristatum has a certain effect of reducing blood fat.

Description

technical field [0001] The invention relates to microorganism cultivation and fermentation, in particular to a method for preparing melanin by the fermentation of Cystis coronoidis. Background technique [0002] Melanin is currently one of the most widely used biological natural pigments. Melanin is mainly a compound with irregular structure formed by oxidation of polyhydroxyphenol, polyhydroxyindole and pyrrole. It often exists in cells in combination with proteins and other substances, and is slightly soluble. Or hardly soluble in water, insoluble in acid solutions and common organic solvents, soluble in alkaline solutions. Natural melanin includes two categories, one is melanin, the final product obtained through biological metabolism of tyrosine and polyphenolic compounds; the other is melanin, which is darker in color from anthocyanins, and is mainly distributed in plants. According to the content of nitrogen and sulfur elements in melanin, melanin can be divided into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/10C12N1/14C09B67/54C09B61/00C12R1/645
CPCC12P17/10C12N1/14C09B67/0096C09B61/00
Inventor 张国广费鹏黄冰晴
Owner MINNAN NORMAL UNIV
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