Method for constructing novel chimeric antigen receptor targeting dual target points of GPC3 and CD19
An antigen and single-chain antibody technology, applied in the field of cell therapy, can solve the problem that liver cancer cannot be cured.
Pending Publication Date: 2020-05-05
HRAIN BIOTECHNOLOGY CO LTD
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Problems solved by technology
The invention relates to a chimeric antigen receptor targeting dual target points of GPC3 and CD19, and the purpose of the chimeric antigen receptor, and particularly provides a polynucleotide sequence. The polynucleotide sequence is selected from (1) a polynucleotide sequence containing an MASK coding sequence, a coding sequence of a GPC3 resistant single-chain antibody, a coding sequence of a CD19 resistant single-chain antibody, a coding sequence of a human IgG4 hinge region, a coding sequence of a human CD28 transmembrane region, a coding sequence of a human 41BB intracellular region, a coding sequence of a human CD3 zeta intracellular region, and a coding sequence of any fragments of EGFR containing ectodomain III and ectodomain IV; and (2) a sequence which is complementary with the polynucleotide sequence in the (1). The invention also provides pertinent fusion protein, a carrier containing the coding sequences, and the purposes of the fusion protein, the coding sequences and thecarrier. The prepared CART cells disclosed by the invention have a tEGFR assembly which has the effects of in vivo tracing and safely switch.
Mammal material medical ingredientsImmunoglobulins +12
- Experimental program(3)
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