Method for constructing novel chimeric antigen receptor targeting dual target points of GPC3 and CD19
An antigen and single-chain antibody technology, applied in the field of cell therapy, can solve the problem that liver cancer cannot be cured.
Pending Publication Date: 2020-05-05
HRAIN BIOTECHNOLOGY CO LTD
7 Cites 0 Cited by
AI-Extracted Technical Summary
Problems solved by technology
View more
Abstract
The invention relates to a chimeric antigen receptor targeting dual target points of GPC3 and CD19, and the purpose of the chimeric antigen receptor, and particularly provides a polynucleotide sequence. The polynucleotide sequence is selected from (1) a polynucleotide sequence containing an MASK coding sequence, a coding sequence of a GPC3 resistant single-chain antibody, a coding sequence of a CD19 resistant single-chain antibody, a coding sequence of a human IgG4 hinge region, a coding sequence of a human CD28 transmembrane region, a coding sequence of a human 41BB intracellular region, a coding sequence of a human CD3 zeta intracellular region, and a coding sequence of any fragments of EGFR containing ectodomain III and ectodomain IV; and (2) a sequence which is complementary with the polynucleotide sequence in the (1). The invention also provides pertinent fusion protein, a carrier containing the coding sequences, and the purposes of the fusion protein, the coding sequences and thecarrier. The prepared CART cells disclosed by the invention have a tEGFR assembly which has the effects of in vivo tracing and safely switch.
Application Domain
Mammal material medical ingredientsImmunoglobulins +12
Technology Topic
CD3Ectodomain +15
Image
Examples
- Experimental program(3)
Example Embodiment
Example Embodiment
Example Embodiment
PUM


Description & Claims & Application Information
We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.