In-vitro culture method for T lymphocytes

A lymphocyte, in vitro culture technology, applied in animal cells, vertebrate cells, blood/immune system cells, etc., can solve the problems of inconvenient use, high cost, unfavorable medical research and clinical application, etc. Reduced requirements for transport conditions and the effect of reducing accidental infections
CN102433303AActive Publication Date: 2012-05-02LIAONING MEDI BIOTECH CO LTD
2 Cites 19 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
LIAONING MEDI BIOTECH CO LTD
Publication Date
2012-05-02

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an in-vitro culture method for T lymphocytes, and radically solves the problems of high cost, inconvenience of use and unfavorable medical research and clinical application existing in the conventional in-vitro culture method for the T lymphocytes. The method comprises the following steps of: directionally sorting the T lymphocytes, inducing, differentiating, culturing, amplifying and the like. A special cell factor combination is used for performing induction differentiation on the T lymphocytes; three factors are a CD3 monoclonal antibody, gamma-interferon and interleukin-1 alpha respectively; the optimal concentration ratio of the three factors is 10:1:1; the total final concentration of the three factors in a culture medium is 1,200ng / ml; and the experiment shows that the combination is an extremely effective way of inducing the T lymphocytes to differentiate.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to a method for treating mononuclear cells in human peripheral blood, umbilical cord blood, pleural effusion, ascites and other samples to become cytokine-induced killer cells (Cytokine-Induced Killer, referred to as CIK), especially a method for The method for culturing T lymphocytes in vitro can be applied to clinical cell biotherapy, basic medical research, clinical application research and biotechnology research, especially immune system research and tumor killing effect research. Background technique

[0002] As tumors pose an increasingly serious threat to human survival and health, the treatment models for tumors are also changing rapidly. Various new drugs, new technologies, and new methods emerge in an endless stream. Among them, cell biology therapy is developed after surgery, radiotherapy, and chemotherapy. The fourth type of tumor treatment method has become an important development direction in tumor biologica...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More