Kit for quantitatively detecting content of pregnancy-related protein A

Abstract

The invention discloses a kit for quantitatively detecting the content of pregnancy-related protein A. The kit comprises a kit body, a sealing cover, a protective cover and a reagent, wherein a solidphase carrier hole, a sample hole, a detection antibody hole, a fluorescein hole, a washing hole and a reading hole are sequentially formed in the kit body; the kit contains all reagent components required for completing immunodetection; the end part of the solid phase carrier loaded in the kit is coated with a capture antibody, and the solid phase carrier is immersed into each hole in the kit sothat the combination of a target antigen in a detected sample and subsequent detection steps can be completed, and the manufacturing and maintenance costs are greatly reduced; the kit is high in detection result accuracy, good in linear relation and high in specificity, and reliable clinical reference value can be provided for early screening of Down's syndrome and early prediction of atherosclerotic plaque rupture.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a kit for quantitatively detecting the content of pregnancy-related protein A. Background technique [0002] Pregnancy-associated protein A (PAPP-A) is a macromolecular glycoprotein derived from the placenta. It is synthesized by placental syncytiotrophoblast cells, decidual cells and endometrial stromal cells in normal menstrual cycle and secreted into serum. Increases with increasing gestational age until full term. It has been proved that the concentration of PAPP-A in the serum of pregnant women with Down syndrome (DS) fetuses in the first trimester is significantly reduced, so PAPP-A is widely recognized as an effective indicator for DS screening, and its concentration reduction indicates that the newborn has Down syndrome. increased risk of Syndrome. [0003] At present, there are data showing that another form of PAPP-A protein is abundantly present in unstable coronary...

AI Technical Summary

Problems solved by technology

ELISA method has many detection steps, takes a long time, and there are many influencing factors in the operation process, which can easily cause false positive and false negative results

Therefore, it is gradually replaced by the chemiluminescence method, but this method is a fully closed system, which is expensive and requires special training for instrument users. The maintenance and testing costs are high, and it is not suitable for single-person and small-batch testing. It is not conducive to PAPP at present. -A detection is widely carried out in China

[0005] In view of the fact that there is no rapid and accurate method for the quantitative detection of PAPP-A, the purpose of the present invention is to provide an immunochromatographic test strip that can be used for the rapid quantitative detection of PAPP-A, for evaluating and monitoring pregnancy-associated protein A in vivo The reagent has the advantages of simple operation, convenience, economy, accurate quantification, etc., and is more suitable for widely used in various medical institutions.

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