Composition for controlling pine wood nematode disease containing acibenzolar-s-methyl as an active ingredient and method for controlling pine wood nematode using the same
A technology of pine wood nematode disease and acibenzolar, which is applied in the field of compositions for preventing and controlling pine wood nematode disease, can solve the problems of undisclosed pine wood nematode disease control technology and achieve the effect of increasing possibility
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Embodiment 1
[0038] Example 1. Screening for inducing resistance substances for preventing and treating pine wood nematode disease
[0039] 16 kinds of substances known to induce resistance to various diseases in plants were used as candidate groups, and the control effect of pine wood nematode was investigated. Using biennial pine seedlings (about 5.5 mm in diameter and about 35 cm in height), an experiment on the control effect of pine wilt nematode in the in vitro test using pine tree pine was carried out.
[0040] As the candidate group for inducing resistance substances, acitidine-S-methyl, Tiadinil (TDL), Isotianil (ITL), Thiabendazole (Probenzole, PBZ), Azuridine Azoxystrobin, AZO, Validamycin A, VMA, Methyl jasmonate, MeJA, DL-3-aminobutyric acid, BABA, 2,3- Butanediol (2,3-Butanediol, 2,3-BDO), r-aminobutyric acid (GABA), dextran (Glucan), ergosterol (Ergosterol, ER) and azelaic acid (Azelaic acid, AZA). Medicament injection is injected with the trunk injection method, and its ...
Embodiment 2
[0046] Example 2. Identification of nematocides by acibenzolar-S-methyl in vitro
[0047] It was investigated whether or not the nematicidal activity of acibenzolar-S-methyl, which showed the most excellent effect in Example 1, was active. For the nematicidal activity test of acibenzolar-S-methyl, the B. xylophilus suspension was diluted at a level of 50 strips / 99 μl per well on a 96-well microplate. A stock solution (stock solution) of 100 times the treatment concentration was prepared in acetone solvent, and 1 μl was added to obtain 300 μg / ml, 100 μg / ml and 33.3 μg / ml respectively. Each treatment area was repeated three times, and 2.15% chemical pesticide Emamectin (Emamectin benzoate) was used in the control area. After treatment, the 96-well microplates were observed after 3 days of storage in a dark room as follows: a wypall soaked in water was spread on the bottom of the plastic box to maintain 100% humidity, and the box was covered with a black cloth to block light . ...
Embodiment 3
[0050] Example 3. In vivo (in vivo) pine wood nematode control effect of the treatment method according to acibenzolar-S-methyl in the greenhouse
[0051] The control effects of three different treatment methods of trunk injection, foliar spraying and soil irrigation of the most active acitrin-S-methyl in Example 1 were investigated. As the pine tree, the same biennial red pine seedlings (about 5.5 mm in diameter and about 35 cm in height) as in Example 1 were used.
[0052] The injection method of the trunk injection is as follows: a drill with a thickness of 1.5mm is drilled at a 45° angle from the trunk of the pine tree at a distance of 5cm to 10cm from the ground surface, and a 200 μl tip is inserted into the hole. After S-methyl was dissolved in an aqueous solution containing 10% acetone and 10% methanol at levels of 0.2 mg / ml and 10 mg / ml and loaded into the tip, 100 µl was injected per pine tree. After processing, the rear of the tip is closed with Parafilm and a small...
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