Oil-in-water adjuvant used for animal vaccines and preparation method for oil-in-water adjuvant

Abstract

The invention provides an oil-in-water adjuvant used for animal vaccines and a preparation method for the oil-in-water adjuvant, and belongs to the technical field of biological product-like animal vaccines. The oil-in-water adjuvant used for the animal vaccines comprises the following raw materials by weight percent: 80.7%-89.5% of mineral oil, 2.1%-2.5% of span 80, 3.0%-5.3% of tween 85, 1.5%-3.5% of lauroyl macrogolglycerides, 0.5%-4.7% of polyglyceryl-6 caprylate and 2.2%-3.6% of polyoxyethylated castor oil. The oil-in-water adjuvant is simple and convenient to prepare, easy to obtain andlow in cost; prepared vaccines are low in viscosity, easy and safe to inject, stable in quality and small in grain size, can reach a nanoscale, and can induce temporary and permanent immunity; and theoil-in-water adjuvant is a safe adjuvant which can be used for poultry and livestock vaccines.

Description

technical field [0001] The invention relates to the technical field of biological product animal vaccines, in particular to an oil-in-water adjuvant for animal vaccines and a preparation method thereof. Background technique [0002] Adjuvants are also known as immune adjuvants in immunology and biological products. The traditional concept is that when a substance is mixed or injected into an animal at the same time before the antigen, it can non-specifically change or enhance the body's specific immune response to the antigen. , a substance that exerts its auxiliary function. The latest concept is any substance that can enhance the antigen-specific immune response. Its function characteristics are: ① It can obviously enhance the weak antigenic substances such as polysaccharides or polypeptides to induce the body to produce a specific immune response; ② Use the least amount of antigen and the least amount of antigen. The number of inoculations stimulates the body to produce ...

AI Technical Summary

Problems solved by technology

At present, imported oil-in-water adjuvants are widely used in the market. Imported adjuvants are expensive and have a long delivery period, so they are not easy to obtain; some vaccine manufacturers also prepare their own adjuvants to prepare vaccines, usually dissolving hydrophilic emulsifiers in antigens, The lipophilic emulsifier is dissolved in mineral oil, and the oil phase and the water phase are mixed and emulsified to prepare vaccines. The preparation process is complicated, which is only suitable for the production and preparation of vaccines by the company, and cannot be widely used; at the same time, some new water-based adjuvants have appeared to replace traditional water-based adjuvants Oil adjuvant, such as Sepic water-based adjuvant Montanide TM GEL, the adjuvant used to prepare vaccines is actually a process of diluting the antigen. Usually, the adjuvant accounts for about 10% of the vaccine, and the amount of antigen used is large, which increases the cost of the vaccine; the antigen diluent in the water-based adjuvant often needs to be added Immunopotentiator, mostly used for dilution of attenuated freeze-dried vaccines; aluminum gel adjuvant is also a commonly used water-based adjuvant in the past, but it has a large side effect after the vaccine is injected into animals, so it is rarely used

[0005] The aqueous adjuvant invented by patent CN201510153978.6 contains Montanide in its components TM GEL 01 needs to rely on imported adjuvants for preparation; the aqueous adjuvant invented by patent CN201710597663.X contains bacterial DNA, capsular polysaccharide, etc. in its components, which requires high preparation technology, contains biological components, and has harsh transportation and storage conditions; The oil-in-water adjuvant invented by patent CN201610908926.X has the appearance of an emulsion, and the preparation requires high-pressure homogenization, filter sterilization, and storage in a nitrogen-filled tank. The preparation and storage methods are relatively complicated; patent CN108704130 .A The compound water adjuvant proposed by A consists of compound immunopotentiators, traditional Chinese medicine immunopotentiators, biological immunopotentiators, multi-components, and a large composition span. Human and biological enhancers are not easy to prepare, and the purity of raw material components, cost and output are not easy to control

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