Non-denatured collagen from yak skin and preparation method of non-denatured collagen

A skin collagen, non-denatured technology, applied in the field of non-denatured yak skin collagen and its preparation, can solve the problems of reducing biological functionality, destroying the triple helix structure, and difficult large-scale production, so as to improve the purity and simplify the pretreatment , the effect of low impurity content

Pending Publication Date: 2020-05-08
青海瑞肽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the collagen sold on the market are collagen peptides prepared by high temperature hydrolysis combined with enzymatic hydrolysis. At this time, the original triple helix structure of collagen has b

Method used

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  • Non-denatured collagen from yak skin and preparation method of non-denatured collagen
  • Non-denatured collagen from yak skin and preparation method of non-denatured collagen
  • Non-denatured collagen from yak skin and preparation method of non-denatured collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method of non-denatured yak skin collagen, the steps are as follows:

[0035] (1) Pretreatment: Mechanically depilate about 500g of fresh yak hide, remove the subcutaneous fat, then wash it with clean water, drain the surface water, cut it into small pieces with a size of about 0.5cm×0.5cm, and put it into- Store in refrigerator at 20°C for later use;

[0036] (2) Degreasing and removing impurities: add the cut yak skin fragments into 2.5L of 5% soda ash solution for soaking, stir at low temperature and low speed at 4°C, and after degreasing for 12 hours, wash with distilled water until the pH is neutral; then degrease Add 4L of 5% sodium chloride solution to the yak skin and continue to soak, stir at low temperature and low speed at 4°C, and after 5 hours, wash with distilled water until the pH is neutral;

[0037] (3) Acidification and homogenization: add the yak skin fragments obtained above after degreasing and removing impurities into 2.5 L of acetic...

Embodiment 2

[0044] A preparation method of non-denatured yak skin collagen, the steps are as follows:

[0045] (1) Pretreatment: Mechanically depilate about 1kg of fresh yak hide, remove the subcutaneous fat, then wash it with clean water, drain the surface water, cut it into small pieces with a size of about 0.5cm×0.5cm, and put it into- Store in refrigerator at 20°C for later use;

[0046] (2) Degreasing and removing impurities: add the cut yak skin fragments to 10L of 3% soda ash solution for soaking, stir at low temperature and low speed at 4°C, and after degreasing for 8 hours, wash with purified water until the pH is neutral; then degrease Add 10L of 3% sodium chloride solution to the yak skin and continue soaking, stir at low temperature and low speed at 4°C, and after 10 hours, wash with distilled water until the pH is neutral;

[0047] (3) Acidification and homogenization: add the yak skin fragments obtained above after degreasing and removing impurities into 10 L of acetic acid s...

Embodiment 3

[0054] A preparation method of non-denatured yak skin collagen, the steps are as follows:

[0055] (1) Pretreatment: Mechanically depilate about 1.5kg of fresh yak hide, remove the subcutaneous fat, then wash it with clean water, drain the surface water, cut it into small pieces with a size of about 0.5cm×0.5cm, put them in Store in -20°C refrigerator for later use;

[0056] (2) Degreasing and removing impurities: soak the cut yak skin fragments in 10L of 5% soda ash solution (W / V), stir at low temperature and low speed at 4°C, and after degreasing for 8 hours, wash with purified water until the pH is neutral. sex. Then add 10 L of 3% sodium chloride solution to the degreased yak hide to continue soaking, stir at low temperature and low speed at 4°C, and wash with distilled water until the pH is neutral after 8 hours;

[0057] (3) Acidification and homogenization: add the yak skin fragments obtained above after degreasing and removing impurities into 15 L of acetic acid solu...

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Abstract

The invention discloses a preparation method of non-denatured collagen from yak skin. The preparation method comprises the following steps: (1) pretreatment; (2) degreasing and impurity removal; (3) acidification and homogenization; (4) enzymolysis; (5) salting-out purification: adding saturated saline to supernatant obtained from step (4), continuously performing stirring until no white flocculesare continuously separated out, and then conducting standing and centrifuging and collecting precipitates; (6) isoelectric point purification: dissolving the precipitates obtained in step (5) with anacetic acid solution, then adjusting the pH of the solution to 6.5-7.0 by using NaOH, conducting standing and centrifuging, and collecting precipitates; (7) desalting; and (8) drying. The method of the present invention overcomes the disadvantages of damage on the triple helix structure of collagen caused by traditional collagen preparation processes. By adopting low-temperature preparation in entire process, the acetic acid, enzymatic hydrolysis, freeze drying and other processes are used to obtain the non-denatured collagen from yak skin. The process of the present invention is simple in operations and capable of achieving large-scale industrial production. The prepared non-denatured collagen from yak skin has good development and utilization prospects in the biomedical fields such as biological dressings and drug delivery carriers.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a non-denatured yak skin collagen and a preparation method thereof. Background technique [0002] Yak is a rare semi-wild endemic population in China and even in the world. It mainly grows in Qinghai-Tibet Plateau, Qinghai and other areas with clear water, blue sky and lush grass. The pastures eaten are also mixed with various precious Chinese herbal medicines. In such an environment, yaks are not only delicious but also full of treasures. Among them, yak hide is the unique treasure house of high-quality collagen extraction in the world. However, at present, yak hide is mainly used for tanning, which seriously reduces The utilization value of yak hide has been improved. [0003] As a natural biomacromolecule, collagen has been widely used in food, biomedicine and cosmetics because of its good biocompatibility, degradability and biological activities such...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/30C07K1/18
CPCC12P21/06C07K14/78
Inventor 雷绍南马青林
Owner 青海瑞肽生物科技有限公司
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