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A kind of method for separating medlar pigment monomer

A wolfberry pigment and monomer technology, applied in the direction of organic chemistry, can solve the problems of cumbersome steps, and achieve the effects of simple preparation process, short extraction and separation time, and easy implementation

Active Publication Date: 2021-04-13
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can obtain zeaxanthin extract with a content of 85% to 95%, only one monomer of zeaxanthin can be obtained, and the steps are cumbersome, requiring repeated treatment with various solvents

Method used

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  • A kind of method for separating medlar pigment monomer
  • A kind of method for separating medlar pigment monomer
  • A kind of method for separating medlar pigment monomer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Concrete extraction method

[0024] A. Crushing

[0025] The wolfberry fruit is crushed and passed through a 20-mesh sieve to obtain wolfberry powder.

[0026] B. Selection of separation solvent system:

[0027] During countercurrent chromatographic separation, the selection of a two-phase solvent system for separation is a very important step. Usually, after mixing different solvents, an appropriate solvent system is selected according to the distribution coefficient of the target compound between the upper phase and the lower phase in the formed two-phase solvent system. The present invention compares the distribution coefficients of zeaxanthin, zeaxanthin monopalmitate and zeaxanthin dipalmitate in two-phase solvent systems composed of different solvents, and some solvent system screening results are as follows:

[0028] Solvent system (v / v) K 1

K 2

K 3

n-Hexane-Dichloromethane-Acetonitrile (10:3.5:6.5) 0.26 1.59 20.11 ...

Embodiment 2

[0039] A. Crushing

[0040] The wolfberry fruit is crushed and passed through a 20-mesh sieve to obtain wolfberry powder.

[0041] B. Selection of separation solvent system:

[0042] Mix n-hexane, dichloromethane, and acetonitrile according to the volume ratio of 9:3.5:6, shake vigorously and then stand for stratification. The upper and lower phases are separated and used as the stationary phase and mobile phase for countercurrent chromatography.

[0043] C, the extraction of wolfberry pigment

[0044] Weigh 10g of wolfberry sample pulverized in step A, add 50mL of the upper phase solvent in step B as the extraction solvent, and extract by high-speed shearing, the shearing time is 8min, and the shearing speed is 16000rpm. The extract was directly separated by countercurrent chromatography after filtration.

[0045] D. Countercurrent chromatographic separation

[0046] Use the upper phase of the solvent system prepared in step B as the stationary phase, and the lower phase ...

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Abstract

The invention provides a method for separating wolfberry pigment monomers, that is, a method for separating three wolfberry pigment monomers in wolfberry, zeaxanthin, zeaxanthin monopalmitate and zeaxanthin dipalmitate. Through the method, three medlar pigment monomers of zeaxanthin, zeaxanthin monopalmitate and zeaxanthin dipalmitate can be obtained with a purity greater than 85%. The separation cost of the present invention is low, and the preparation process is safe. By combining the high-speed shear rapid extraction technology and the countercurrent chromatography high-efficiency separation technology, the countercurrent chromatography is used to separate the two-phase solvent as the high-speed shear extraction solvent, and the extract is directly filtered after extraction. Separation by countercurrent chromatography saves intermediate steps, and can rapidly separate and prepare three kinds of wolfberry pigment monomers of zeaxanthin, zeaxanthin monopalmitate and zeaxanthin dipalmitate with a purity of more than 85% from wolfberry.

Description

technical field [0001] The invention belongs to the technical field of separation of natural products, and in particular relates to a method for separating wolfberry pigment monomers, that is, a method for separating three wolfberry pigment monomers in wolfberry, zeaxanthin, zeaxanthin monopalmitate and zeaxanthin dipalmitate . Background technique [0002] Lycium barbarum pigment is a natural colorant extracted from the fruit of Lycium Barbartum L., which is mainly used in food and cosmetics. Its main components are fat-soluble carotenoids and their esters. Among them, zeaxanthin, zeaxanthin monopalmitate and zeaxanthin dipalmitate are three kinds of pigment monomers with higher content in wolfberry pigment. Zeaxanthin and its esters have strong antioxidant activity, which can protect biological systems from peroxidative damage by reducing the reactivity of free radical singlet oxygen and photochemical sensitizers. At the same time, zeaxanthin and its esters also have the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
CPCC07C403/24C07C2601/16
Inventor 黄新异邸多隆巩媛
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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