Normal-temperature low-pressure normal-oxygen liver perfusion system

Abstract

A practical normal-temperature low-pressure normal-oxygen liver perfusion system for small animals is characterized in that a peristaltic pump extrudes a portal vein tube and a hepatic artery tube toprovide circulating power, a tube diameter with a proper inner diameter is selected to enable the perfusion flow ratio of a portal vein and a hepatic artery to be 3: 1, and an animal breathing machinesupplies oxygen to the hepatic arteries through a membrane oxygenator; an isolated liver is placed in an organ perfusion box, the portal vein and the hepatic artery are perfused through a portal veincannula and a hepatic artery cannula respectively, and a liquid outlet is connected with a hepatic vein cannula and a biliary tract cannula; a physiological recorder is connected with the portal veincannula through a medical tee joint and perfused through a portal vein low-pressure control system; a serpentine condenser is adopted for system temperature control and connected with a water bath kettle for water circulation, and the liquid outlet of the organ perfusion box is connected with a blood transfusion apparatus filter and then connected with a liquid inlet pipe of the peristaltic pumpthrough the serpentine condenser and a hose; and the perfusate adopts an anticoagulant animal blood component. In conclusion, the system is simple in design structure, conforms to physiological perfusion, is low in cost and is used for in-vitro research and functional repair of the liver.

Description

technical field [0001] The invention belongs to the field of medical technology, and in particular relates to a normal temperature and low pressure normoxia liver perfusion system. Background technique [0002] With the maturity of liver transplantation technology, liver transplantation has become an effective method for the treatment of end-stage liver diseases. In order to solve the severe shortage of donor organs, expanding the source of organs and increasing the use of marginal donors such as donor aftercardiac death (DCD) have become the development trend of clinical liver transplantation at home and abroad. Compared with organ donation from donors after brain death (DBD), the main factor affecting the liver quality of DCD donors is warm ischemic injury. Warm ischemia time longer than 20-30 minutes is the main cause of delayed recovery of liver function and low survival rate after DCD liver transplantation, and the incidence of complications such as hepatic artery embo...

AI Technical Summary

Problems solved by technology

[0004] The research and equipment development of liver mechanical perfusion at home and abroad is in the ascendant, and the indications, perfusion routes, perfusate components, perfusion temperature, flow rate and pressure parameters of mechanical perfusion application need to be further explored and determined. Experimental research on animal models; the existing devices are inconvenient to use and have incomplete functions. Research on liver mechanical perfusion and equipment development are in the ascendant at home and abroad, and there is no unified standard. Does not correspond to physiology, can not fully simulate liver perfusion in physiological state

Images