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Method for extracting Silybum marianum (L.) Gaertn protein from Silybum marianum (L.) Gaertn pharmaceutical residues

A milk thistle and water extraction technology, applied in the field of protein extraction, can solve the problems of difficult protein extraction, low utilization rate of milk thistle pharmaceutical dregs, etc., and achieve the effect of improving extraction efficiency

Active Publication Date: 2020-05-19
DALIAN UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to propose a method for extracting milk thistle protein from milk thistle pharmaceutical dregs, which can effectively extract Protein, while stabilizing protein purity, extraction rate and actual yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This example discloses a method for extracting milk thistle protein from milk thistle pharmaceutical dregs, specifically, including the following steps:

[0032]Step (1) After recovery and concentration of milk thistle pharmaceutical dregs, carry out vacuum drying treatment, under the condition of relative vacuum degree -0.1Mpa, vacuum dry for 6h, remove ethyl acetate, acetone or n-hexane in the pharmaceutical extraction process solvent residue;

[0033] Step (2) The dried milk thistle dregs in step (1) are ground and pulverized through a 10-mesh sieve by a pulverizer to obtain milk thistle dregs powder with a particle size not greater than 2 mm;

[0034] Step (3) add 0.03g NaOH per gram of milk thistle dregs powder, mix well, then add 0.005g vitamin C, mix well, then add 10mL pure water, stir well, and extract at a constant temperature at 20°C, And keep stirring or shaking for 2h;

[0035] Step (4) centrifuge the leaching suspension for 5 minutes at 4000 rpm to obtai...

Embodiment 2

[0042] This example discloses a method for extracting milk thistle protein from milk thistle pharmaceutical dregs, specifically, including the following steps:

[0043] Step (1) After recovery and concentration of milk thistle pharmaceutical dregs, carry out vacuum drying treatment, under the condition of relative vacuum degree -0.1Mpa, vacuum dry for 12h, remove ethyl acetate, acetone or n-hexane in the pharmaceutical extraction process solvent residue;

[0044] Step (2) The dried milk thistle dregs in step (1) are ground and pulverized through a 10-mesh sieve by a pulverizer to obtain milk thistle dregs powder with a particle size not greater than 2mm;

[0045] Step (3) add 0.04g NaOH per gram of milk thistle dregs powder, mix well, then add 0.01g vitamin C, mix well, then add 10mL pure water, stir well, and extract at 40°C at a constant temperature, And keep stirring or shaking for 3h;

[0046] Step (4) centrifuge the leaching suspension for 10 min at 8000 rpm to obtain t...

Embodiment 3

[0053] This example discloses a method for extracting milk thistle protein from milk thistle pharmaceutical dregs, specifically, including the following steps:

[0054] Step (1) After recovery and concentration of milk thistle pharmaceutical dregs, carry out vacuum drying treatment, under the condition of relative vacuum degree -0.1Mpa, vacuum dry for 24 hours, remove ethyl acetate, acetone or n-hexane in the pharmaceutical extraction process solvent residue;

[0055] Step (2) The dried milk thistle dregs in step (1) are ground and pulverized through a 10-mesh sieve by a pulverizer to obtain milk thistle dregs powder with a particle size not greater than 2mm;

[0056] Step (3) Add 0.05g NaOH to each gram of milk thistle dregs powder, mix well, then add 0.02g vitamin C, mix well, then add 10mL pure water, stir well, and extract at a constant temperature at 50°C, And keep stirring or shaking for 3h;

[0057] Step (4) centrifuge the leaching suspension for 20 min at 10,000 rpm ...

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Abstract

The invention provides a method for extracting Silybum marianum (L.) Gaertn protein from Silybum marianum (L.) Gaertn pharmaceutical residues. The method includes the following steps: performing vacuum drying on the Silybum marianum (L.) Gaertn pharmaceutical residues, performing grinding so as to obtain residue powder, adding NaOH, vitamin C and purified water to the residue powder, performing constant-temperature leaching, performing centrifugation so as to obtain a leaching supernatant I and leaching residues I, adding hydrochloric acid to the leaching supernatant I so as to adjust the pH value, performing still standing for precipitation, conducting centrifugation so as to obtain a protein precipitate I, performing second leaching on the leaching residues I, performing centrifugation on the leaching turbid liquid so as to obtain an extract supernatant II, adding hydrochloric acid to the extract supernatant II so as to adjust the pH value, performing precipitation and centrifugationso as to obtain a protein precipitate II, merging the protein precipitate I and protein precipitate II so as to obtain a total protein extract, and performing freeze-drying on the total protein extract so as to obtain crude protein powder of Silybum marianum (L.) Gaertn. The yield of protein of Silybum marianum (L.) Gaertn, recovery rate of total protein and purity of protein can be improved, andan optimized preparation process is provided for extraction of Silybum marianum (L.) Gaertn protein from the Silybum marianum (L.) Gaertn pharmaceutical residues.

Description

technical field [0001] The invention relates to protein extraction technology, in particular to a method for extracting milk thistle protein from milk thistle pharmaceutical dregs. Background technique [0002] Milk thistle is a herbaceous plant of the genus Milk thistle in the Compositae family. As a traditional plant, silymarin extracted from milk thistle seeds has good liver protection, anti-oxidation, and inhibition of cancer cells. Favored in the health care market. Milk thistle dregs are the residues after silymarin extraction. The traditional method of silymarin dregs is mainly used as protein feed for livestock and poultry, and the output value and profit are low. However, milk thistle dregs contain relatively high vegetable protein, accounting for about 5-10% of the total dry weight of dregs, and milk thistle protein has a complete range of amino acids and is a better source of vegetable protein. The annual output of milk thistle dregs in my country is about 2,000...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K1/30
CPCC07K1/145C07K1/30Y02P60/87
Inventor 徐卫平路延卫鑫陈继红王大超许建强陈金龙
Owner DALIAN UNIV OF TECH