Composition for adsorbing impure protein of antibody and preparation method of composition

Abstract

The invention relates to a composition for adsorbing impure proteins of an antibody and a preparation method of the composition. The composition comprises the following components in parts by weight:15-30 parts of cane sugar, 15-20 parts of xylan, 15-10 parts of D-oligosaccharide, 10-30 parts of galactose, 10-20 parts of fructooligosaccharide and 10-20 parts of maltose. The composition provided by the invention has the advantages of being definite in treatment effect, convenient to use, low in price and easy to store; impure proteins, macromolecule proteins and proteins without immunogenicityin antibodies can be adsorbed, then an antibody product can be pure, the titer can be stable, and the immunogenicity is good; while the titer of the antibody is improved through adsorption and concentration, immune globulin specific antibodies can be increased; non-specific serum proteins can be reduced; IgG in the serum immune globulin can be purely separated, the purity can be as high as 75%, homogenized immune globulin parts can be extracted from physical and chemical properties, impure proteins can be removed, and the content of the immune globulin can be increased; and therefore, the composition has a very good clinical treatment effect on Fabricius bursae, flu and Newcastle diseases of chicken communities, and good immune uniformity is achieved.

Description

technical field [0001] The invention belongs to the field of biological products, in particular to a composition for absorbing antibody miscellaneous proteins and a preparation method thereof. Background technique [0002] With the development of China's aquaculture industry, group, large-scale, and intensive aquaculture have become popular, and diseases are becoming more and more rampant. Especially in recent years, the outbreak rate of viral diseases is high, the cure rate is low, and the mortality rate continues to rise. , has brought great economic losses to farmers. Although vaccines and antibodies have been widely used in breeding bases with the continuous rise of biological product technology in recent years, the safety, timeliness and compatibility of vaccines They are all subject to great restrictions, especially the application of antibodies. The matching of strains is very important; biological antibodies for injection itself are very stressful to chickens, and it...

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Problems solved by technology

[0002] With the development of China's aquaculture industry, group, large-scale, and intensive aquaculture have become popular, and diseases are becoming more and more rampant. Especially in recent years, the outbreak rate of viral diseases is high, the cure rate is low, and the mortality rate continues to rise. , has brought great economic losses to farmers. Although vaccines and antibodies have been widely used in breeding bases with the continuous rise of biological product technology in recent years, the safety, timeliness and compatibility of vaccines They are all subject to great restrictions, especially the application of antibodies. The matching of strains is very important; biological antibodies for injection itself are very stressful to chickens, and it is easy to cause cross-contamination. If the effect is inaccurate, the gain outweighs the gain. Purification and separation of more immunogenic globulins is the focus of work

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