Bacillus megaterium and application thereof
A technology of Bacillus megaterium and strain, applied in the field of biological fermentation engineering, can solve the problems of weak growth vigor, slow growth rate, low fermentation yield and the like
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[0018] The present application will be further explained below in conjunction with the embodiments. Before introducing the specific embodiments, a brief description of some of the experimental backgrounds involved in the following embodiments is as follows.
[0019] Medium:
[0020] Nutrient Agar medium (Nutrient Agar.NA): peptone 10.00 g, beef extract 3.00 g, sodium chloride 5.00 g, agar 15.0 0 g, distilled water 1.00 L, pH 7.0;
[0021] Beef extract medium (Bee4.3 extract Agar): take 500.00 g of fat-free lean meat, chop it, add 1.00 L of distilled water to a beaker, put it in a refrigerator at 4 °C overnight, filter it with cotton, and boil the filtrate in a water bath 1 h, make the protein coagulate, filter, add 10.00 g peptone and 5.00 g sodium chloride to the filtrate, adjust the pH to 7.4, and make up the liquid to 1.00 L, 15.00 g agar, sterilize after aliquoting, and set aside;
[0022] Method for Determination of Vitality of Bacillus megaterium
[0023] Use the dilu...
Embodiment 1
[0030] The present embodiment provides with regard to the Bacillus megaterium ( Bacillus megaterium ) The process of mutagenesis screening of YSJ3 strain is briefly introduced as follows.
[0031] The bacillus megaterium provided by the application ( Bacillus megaterium ) YSJ3 is mainly obtained through ultraviolet mutagenesis and ion beam mutagenesis screening of the existing starting strain Bacillus megaterium YSJ1. The specific mutagenesis and screening process are briefly described as follows.
[0032] (1) Ultraviolet mutagenesis
[0033] The first is to carry out ultraviolet mutagenesis breeding on the starting strain Bacillus megaterium YSJ1, and the main process is as follows:
[0034] Take 4ml of YSJ1 bacterial suspension cultured to the logarithmic growth phase at 37°C, and irradiate it with a 15 W ultraviolet lamp at a distance of 28 cm. During the irradiation process, the survival rate of the strain gradually decreases with the extension of the ultraviolet irradia...
Embodiment 2
[0052] On the basis of the strain obtained in Example 1, the inventors further optimized the fermentation process to further improve the growth and fermentation capacity of the strain. The specific process is introduced as follows.
[0053] (1) Strain activation and seed solution preparation
[0054] After the preserved Bacillus megaterium YSJ3 strain was activated and cultivated in LB plate medium at 37°C for 2 days, the bacterial lawn was collected and transferred to LB liquid medium for further amplification, and then transferred to the seed medium of the seed tank. Ferment at 37°C and 280rpm for about 16 hours as seed liquid;
[0055] The seed medium is: 2% glucose, 3% corn flour, 5% bean cake powder, 1% corn steep liquor powder, 0.2% potassium dihydrogen phosphate, 1.5% yeast powder, pH 7.0.
[0056] (2) Preparation of fermentation broth
[0057] According to the inoculation amount of 10% volume ratio, the seed liquid prepared in step (1) was transferred to the ferment...
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