Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of Polygonatum curcuma tissue culture method

A tissue culture and leaf-rolling technology of Polygonatum chinensis, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of long time required for reproduction, low survival rate of Polygonatum chinensis, slow growth of seedlings, etc., and achieve disinfection effect. Good, high survival rate, stable quality effect

Active Publication Date: 2022-03-08
SICHUAN AGRI UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies in the prior art, the invention provides a method for tissue culture of Rhizoma Polygonatum, using the tuber of Rhizoma Polygonatum as an explant, and quickly obtaining the seedlings of Rhizoma Polygonatum through tissue culture, so as to realize the rapid propagation of Rhizoma Polygonatum, effectively solving the problem of The problem of low survival rate of Rhizoma Polygonatum, long time required for reproduction, slow growth of seedlings and unstable quality

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Polygonatum curcuma tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A kind of Polygonatum curcuma tissue culture method, comprises the following steps:

[0032] (1) pretreatment: the raw Polygonatum tuber of the year is washed with a toothbrush in running water, then soaked in a washing powder solution of 2vt% for 5min in a concentration, washed with running water, and then soaked in 0.001wt% sodium hypochlorite solution for 8min, Continue to wash with running water, and finally rinse with sterile water for 10 minutes, and dry to obtain the pretreated Polygonatum tuber; wherein, the sodium hypochlorite solution contains 3vt% active oxygen;

[0033] (2) Sterilization: Place the Polygonatum curcuma tuber after the pretreatment obtained in step (1) on the ultra-clean workbench, first soak it with 75vt% ethanol surface for 15s, wash it with sterile water for 3 times, and then put it into 0.05vt% ethanol mercuric chloride solution, and dropwise added 3 drops of 0.1vt% polysorbate-80 solution, sterilized for 10 minutes, continued to rinse wit...

Embodiment 2

[0039] A kind of Polygonatum curcuma tissue culture method, comprises the following steps:

[0040] (1) pretreatment: the raw Polygonatum tuber of the year is washed with a toothbrush in running water, then soaked in a washing powder solution of 2vt% for 8min in a concentration, washed with running water, and then soaked in 0.001wt% sodium hypochlorite solution for 10min, Continue to wash with running water, finally rinse with sterile water for 13 minutes, and dry to obtain the pretreated Polygonatum tuber; wherein, the sodium hypochlorite solution contains 3vt% active oxygen;

[0041] (2) Sterilization: place the Polygonatum curcuma tuber after the pretreatment obtained in step (1) on the ultra-clean workbench, first soak the surface with 75vt% ethanol for 25s, rinse 4 times with sterile water, and then put in 0.1vt% ethanol mercuric chloride solution, and dropwise added 3 to 6 drops of 0.15vt% polysorbate-80 solution, sterilized for 20 minutes, continued to rinse with steril...

Embodiment 3

[0047] A kind of Polygonatum curcuma tissue culture method, comprises the following steps:

[0048] (1) pretreatment: the raw Polygonatum tuber of the year is cleaned with a toothbrush in running water, then soaked in a washing powder solution of 6vt% for 10min in a concentration, washed with running water, and then soaked in 0.001wt% sodium hypochlorite solution for 12min, Continue to wash with running water, finally rinse with sterile water for 15 minutes, and dry to obtain the pretreated Polygonatum tuber; wherein, the sodium hypochlorite solution contains 3vt% active oxygen;

[0049] (2) Sterilization: place the Polygonatum curcuma tuber after the pretreatment obtained in step (1) on the ultra-clean workbench, first soak the surface with 75vt% ethanol for 30s, rinse 5 times with sterile water, and then put in 0.2vt% ethanol mercuric chloride solution, and dropwise added 6 drops of 0.2vt% polysorbate-80 solution, sterilized for 30 minutes, continued to rinse with sterile wa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a tissue culture method of Polygonatum curcuma, which comprises the following steps: washing the tuber of Polygonatum curcuma in running water, then soaking it in washing powder solution, soaking it in sodium hypochlorite solution after washing in running water, rinsing it with sterile water, and drying it in the air dry, to obtain the pretreated Polygonatum tuber; sterilize with ethanol surface, rinse, put into mercuric chloride solution to sterilize, rinse, and dry the water to obtain the sterilized Polygonatum tuber; cut into small pieces, and then Put it into the differentiation medium, and cultivate the differentiated Polygonatum adventitious buds; insert it into the proliferation medium, and cultivate the Polygonatum adventitious bud clusters; cut the buds into pieces, insert them into the rooting medium, and cultivate the Polygonatum seedlings; place them in semi-shading, After disinfection, it is colonized, cultivated to obtain Polygonatum seedlings, and then transplanted to outdoor soil. The invention uses the rhizoma rhizome tuber as an explant, rapidly obtains rhizoma rhizome seedlings through tissue culture, realizes the rapid propagation of rhizoma rhizoma rhizome, effectively solves the problems of low survival rate of rhizoma rhizoma rhizome, long time required for propagation, slow growth of seedlings and unstable quality .

Description

technical field [0001] The invention relates to the technical field of tissue culture of Polygonatum curcuma, in particular to a method for tissue culture of Polygonatum curcuma. Background technique [0002] Polygonatum is a commonly used Chinese medicinal material in my country, and it is the general name of the rhizome of perennial herb of the genus Polygonatum in the family Liliaceae. Polygonatum chemical components mainly contain sugars, flavonoids and anthraquinones, steroidal saponins, alkaloids, cardiac glycosides, lignans, vitamins and a variety of amino acids and trace elements useful to the human body; pharmacological studies believe that Polygonatum has Prolong life, anti-aging, affect cardiovascular system, improve immunity, anti-inflammation, anti-pathogenic microorganisms, anti-fatigue, improve learning and memory, inhibit tumor cells, etc., and it is safe, non-toxic, and non-mutagenic. Clinical application studies report that Polygonatum and its preparations...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 段晓宇张良辉杨舒卉
Owner SICHUAN AGRI UNIV