Thin-layer chromatography detection method of nauclea officinalis extract syrup

Abstract

The invention relates to the technical field of thin-layer chromatography detection, in particular to a thin-layer chromatography detection method of nauclea officinalis extract syrup. The method comprises the following steps of: mixing nauclea officinalis extract syrup with ethanol, carrying out reflux heating in a water bath, cooling, filtering, evaporating filtrate to dryness, and adding methanol to obtain a test sample, mixing the nauclea officinalis medicinal material with ethanol, performing reflux heating in a water bath, cooling, filtering, evaporating filtrate to dryness, and adding methanol to obtain a standard substance, respectively spotting the test sample and the standard substance on the same thin-layer plate, developing in a developing solvent, airing, and detecting the color of a fluorescent spot under ultraviolet light, the developing solvent being a mixture of dichloromethane, ethyl acetate and methanol. According to the detection method provided by the invention, amethod for thin-layer identification of the product by taking strictosamide as a target object does not exist in national standard or Chinese Pharmacopoeia is realized, the problems of inferior-quality products, counterfeit products and the like of the product are solved, the method is simple and practical, the consumed time is short, the reproducibility, specificity and durability are strong, andthe monitoring time of the product is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of thin-layer chromatography detection, in particular to a thin-layer chromatography detection method for the extract syrup of eucalyptus. Background technique [0002] Gallwood belongs to Rubiaceae Ebony, a woody plant, is a famous southern medicine in my country, also known as medicine ebony, mountain bear bile and yellow bile wood. Gallwood is bitter in taste, cold in nature, has the functions of clearing away heat and detoxification, reducing swelling and relieving pain, etc. Leg ulcers, athlete's foot infection, burn infection, boils, eczema. Danmu has been used as a folk medicine, and it was first included in books such as "Handbook of Commonly Used Chinese Herbal Medicine" and "National Compilation of Chinese Herbal Medicine" compiled by the Guangzhou Army, and it was also included in the 1977 edition of "Chinese Pharmacopoeia". As early as 1977, it was reported that the bile wood preparation succes...

AI Technical Summary

Problems solved by technology

However, the operation of high performance liquid chromatography is cumbersome and requires high equipment conditions, which is not conducive to popularization in the environment where the instruments of the majority of grassroots testing departments in China are backward and the quality of operators is generally low.

[0005] As a chemical separation and analysis method, thin-layer chromatography is simple to operate and has the functions of qualitative identification, quality control and impurity detection. The classic identification of chemistry is to use supersolvent extraction or treatment with reagents to separate or divide them into single The disadvantage of this method is that the sample volume is large, the separation process is troublesome, the analysis time is long, and there may be interference from impurities

There is no good detection method at this stage, and there is no corresponding detection standard in the national standard and "Chinese Pharmacopoeia". It is difficult to guarantee the Questions about the authenticity of paste syrup preparations or adulteration of defective products

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