Method for constructing electrochemical luminescence sensor based on double amplification of perylenetetracarboxylic acid signals of cobalt-based metal organic framework

A technology of organic framework and perylenetetracarboxylic acid, which is applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve problems such as inability to produce, and achieve accurate and reliable results, excellent stability, and stable effects

Active Publication Date: 2020-05-29
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, PTCA by itself cannot generate sufficient ECL emission for trace analysis, which prompted us to find an effective enhancer to improve its ECL signal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A glassy carbon electrode with a diameter of 4 mm was covered with Al 2 o 3 Polish the slurry to obtain a mirror-like surface, and rinse it with ultrapure water; immerse the treated electrode in 1% HAuCl 4 In the solution, a layer of AuNPs was electrodeposited at a constant voltage of -0.2 V, and the deposition time was 30 s; the deposited electrode was immersed in a 50 ng / mL HGC solution for 2 h, and a certain amount of HGC was bound by gold-sulfur bonds; 8 μL of 5 μg / mL Ab 1 Drop-coated on the surface of the electrode, in 4 o Incubate at C; drop 3 μL of 1% BSA onto the surface of the electrode to block the Ab 1 On the non-specific active site; 5 μL of different concentrations of Aβ was drop-coated on the surface of the electrode, 4 o C for 1 h; 8 µL 4 µg / mL Ab 2 -Au-PTCA@ZIF-67 solution was drip-coated on the electrode surface, 4 o After incubating at C for 2 h, the construction of an electrochemiluminescent sensor based on cobalt-based metal-organic frameworks ...

Embodiment 2

[0024] A glassy carbon electrode with a diameter of 4 mm was covered with Al 2 o 3 Polish the slurry to obtain a mirror-like surface, and rinse it with ultrapure water; immerse the treated electrode in 1% HAuCl 4 In the solution, a layer of AuNPs was electrodeposited at a constant voltage of -0.2 V, and the deposition time was 30 s; the deposited electrode was immersed in a 50 ng / mL HGC solution for 2 h, and a certain amount of HGC was bound by gold-sulfur bonds; 8 μL of 5 μg / mL Ab 1 Drop-coated on the surface of the electrode, in 4 o Incubate at C; drop 3 μL of 1% BSA onto the surface of the electrode to block the Ab 1 On the non-specific active site; 5 μL of different concentrations of Aβ was drop-coated on the surface of the electrode, 4 o Incubate at C for 1 h; add 8 µL of 5 µg / mL Ab 2 -Au-PTCA@ZIF-67 solution was drip-coated on the electrode surface, 4 o After incubating at C for 2 h, the construction of an electrochemiluminescent sensor based on cobalt-based metal-...

Embodiment 3

[0026] A glassy carbon electrode with a diameter of 4 mm was covered with Al 2 o 3 Polish the slurry to obtain a mirror-like surface, and rinse it with ultrapure water; immerse the treated electrode in 1% HAuCl 4 In the solution, a layer of AuNPs was electrodeposited at a constant voltage of -0.2 V, and the deposition time was 30 s; the deposited electrode was immersed in a 50 ng / mL HGC solution for 2 h, and a certain amount of HGC was bound by gold-sulfur bonds; 8 μL of 5 μg / mL Ab 1 Drop-coated on the surface of the electrode, in 4 o Incubate at C; drop 3 μL of 1% BSA onto the surface of the electrode to block the Ab 1 On the non-specific active site; 5 μL of different concentrations of Aβ was drop-coated on the surface of the electrode, 4 o C for 1 h; 8 µL of 6 µg / mL Ab 2 -Au-PTCA@ZIF-67 solution was drip-coated on the electrode surface, 4 o After incubating at C for 2 h, the construction of an electrochemiluminescent sensor based on cobalt-based metal-organic framewor...

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Abstract

The invention discloses a method for constructing an electrochemical luminescence sensor based on double amplification of perylenetetracarboxylic acid signals of a cobalt-based metal organic framework. According to the method, perylenetetracarboxylic acid PTCA is loaded on ZIF-67 as a luminous body to form PTCA(at)ZIF-67 as a signal probe, wherein the ZIF-67 is used as a reinforcing agent and cancatalyze S2O8 < 2-> to generate more SO4 <-> so that double amplification of signals is realized. Besides, heptapeptide HGC is introduced to fix the antibody so that the biological activity of the sensor is maintained, the incubation efficiency of the antibody is improved and the sensitivity of constructing the sensor is greatly improved. The beta-amyloid protein A beta with different concentrations can be combined with different amounts of secondary antibody markers Ab2-Au-PTCA (at) ZIF-67 so that the luminous intensity of the sensor is changed and the ultra-sensitive detection of the A betais realized.

Description

technical field [0001] The present invention designs a method for constructing an electrochemiluminescent sensor based on cobalt-based metal-organic framework double amplification of perylene tetracarboxylic acid signal. Specifically, PTCA is used as a luminescent body and ZIF-67 is used as an enhancer to prepare a double signal for detecting Aβ. An amplified electrochemiluminescence sensor belongs to the technical field of electrochemiluminescence detection. Background technique [0002] Alzheimer's disease is a progressive neuromuscular wasting disorder initially characterized by learning and memory impairments that eventually lead to loss of cognitive abilities. Furthermore, it is the most common cause of Alzheimer's disease. Genetic studies have shown that Aβ plays a key role in the development of Alzheimer's disease, and it can be used as an important indicator for monitoring and diagnosing Alzheimer's disease. So far, only a few analytical methods for the quantitativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/305G01N27/308G01N27/3275
Inventor 宋先震魏琴罗川南范大伟任祥孙晓君邵鑫荣
Owner UNIV OF JINAN
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