Novel coronavirus N-S dominant epitope fusion protein, preparation method, application, expression protein, microorganism, application and kit

Abstract

The invention discloses a novel coronavirus N-S dominant epitope fusion protein, a preparation method, application, an expression protein, a microorganism, application and a kit, and belongs to the technical field of genetic engineering. The novel coronavirus N-S dominant epitope fusion protein is a novel coronavirus N protein and S protein dominant epitope fusion protein. The preparation method comprises the following steps: firstly, constructing a fusion expression antigen by an S protein dominant epitope segment antigen and an N protein dominant epitope segment antigen of a novel coronavirus; then removing rare codons through gene optimization to obtain an optimized gene sequence; and finally, obtaining a soluble high-activity antigen with efficient expression. The novel coronavirus N-Sdominant epitope fusion protein disclosed by the invention has soluble expression, high yield, high purity and good activity, and is suitable for accurate diagnosis and detection of patients with thenovel coronavirus.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to the preparation and application of a fusion protein, in particular to a novel coronavirus N-S dominant epitope fusion protein, preparation method, application, expression protein, microorganism, application, and kit. Background technique [0002] Coronaviruses are a large family of viruses known to cause colds as well as more serious illnesses such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). On January 31, 2020, the World Health Organization suggested that the new type of coronavirus pneumonia that appeared in 2019 be temporarily named "2019-nCoV acute respiratory disease", and the virus was temporarily named "2019-nCoV" (2019 represents the year when it first appeared, n means novel (new), CoV means coronavirus (coronavirus)). On February 7, China's National Health and Medical Commission issued a notice, deciding to tempora...

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Problems solved by technology

However, due to the limitation of nucleic acid detection by unfavorable factors such as difficult sampling, complicated operation, and long detection time, some infected persons will be missed (for example, many cases of nucleosides have appeared in the process of using nucleoside detection to detect suspected patients with new coronary pneumonia in my country. The test was negative, but the example of the late diagnosis of the patient had a certain impact on the control of the epidemic)

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