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Cortex dictamni antiviral active part and preparation method, content determination method and application thereof

A technology of antiviral activity and active parts, which is applied in the field of traditional Chinese medicine extracts, can solve the problems of difficult immunity of high-risk groups, easy mutation of influenza virus, and difficulty of group immunity, etc., and achieves easy quality control, clear content, and simple chemical composition clear effect

Inactive Publication Date: 2020-06-05
HARBIN VOCATIONAL & TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main prevention and control measure for influenza virus is vaccination, but the vaccine itself has certain limitations: the immunization rate is low, it is difficult to produce effective herd immunity; it is difficult to produce effective immunity for high-risk groups; and because influenza virus is easy to Mutations, so it is difficult to produce corresponding vaccines in a short period of time in the early stages of the rapid spread of newly mutated viruses

Method used

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  • Cortex dictamni antiviral active part and preparation method, content determination method and application thereof
  • Cortex dictamni antiviral active part and preparation method, content determination method and application thereof
  • Cortex dictamni antiviral active part and preparation method, content determination method and application thereof

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Embodiment 1

[0029] The invention relates to an antiviral active part of white fresh cortex. The total limonoid content of the active part is more than 80%, and contains limonin, phellodendron, alkone, arperidone and pro-phellodendron.

[0030] The application of an antiviral active part of Dictatoria radix Cortex in the preparation of antiviral medicaments.

[0031] Such as figure 1 Shown, a kind of preparation method of Radix Cortex Antiviral Active Site comprises the following steps:

[0032] (1) Extraction: take white fresh bark and crush it, add 10 times the amount of petroleum ether, reflux in a water bath at 70°C for 2 extractions, each time for 1 hour, and combine the filtrates. Filter, add 10 times the amount of dichloromethane to the dregs, extract twice under reflux in a water bath at 85°C, 1 hour each time, filter, and combine the filtrates. Concentrate to obtain concentrated extract.

[0033] (2) Impurity removal: macroporous resin column chromatography, after dissolving th...

Embodiment 2

[0036] An antiviral active part of white fresh cortex, the total limonoid content of the active part is more than 50%, and contains limonin, cortexone, alkone, arperone and pro-cortexone.

[0037]The application of an antiviral active part of Dictatoria radix Cortex in the preparation of antiviral medicaments.

[0038] A preparation method of antiviral active part of Radix Cortex Dictica, comprising the following steps:

[0039] (1) Extraction: take white fresh bark and pulverize it, add 6 times the amount of petroleum ether, reflux in a water bath at 70°C for 2 extractions, each time for 1 hour, and combine the filtrates. Filter, add 8 times the amount of dichloromethane to the dregs, extract twice in a water bath at 85°C under reflux, each time for 1 hour, filter, and combine the filtrates. Concentrate to obtain concentrated extract.

[0040] (2) Impurity removal: macroporous resin column chromatography, after dissolving the concentrated extract obtained from the above ext...

Embodiment 3

[0043] An antiviral active part of white fresh cortex, the total limonoid content of the active part is more than 50%, and contains limonin, cortexone, alkone, arperone and pro-cortexone.

[0044] The application of an antiviral active part of Dictatoria radix Cortex in the preparation of antiviral medicaments.

[0045] A preparation method of antiviral active part of Radix Cortex Dictica, comprising the following steps:

[0046] (1) Extraction: take white fresh bark and pulverize it, add 6 times the amount of petroleum ether, reflux in a water bath at 70°C for 2 extractions, each time for 1 hour, and combine the filtrates. Filter, add 6 times the amount of dichloromethane to the dregs, extract twice under reflux in a water bath at 85°C, 1 hour each time, filter, and combine the filtrates. Concentrate to obtain concentrated extract.

[0047] (2) Impurity removal: Macroporous resin column chromatography, after dissolving the concentrated extract obtained from the above extrac...

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Abstract

The invention relates to a cortex dictamni antiviral active part and a preparation method, content determination method and application thereof. The cortex dictamni antiviral active part with contentof limonoid compound of more than 50% is obtained by using the preparation method, the preparation process is simple, the operation is easy, and the cost is low. Experiments show that the cortex dictamni effective part obtained by the preparation method has very good antiviral effects, and then the fact that the higher the total content of the two compounds of obakunone and fraxinellone is, the stronger the antiviral effect is is further found through experiments. The invention further discloses the content determination method and main component range of the cortex dictamni antiviral active part, so that the promotion and control of quality standards are facilitated in the later development and application.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract with antiviral activity, in particular to an antiviral active part in white fresh cortex, a preparation method, content determination and application thereof. Background technique [0002] Influenza not only poses a great threat to human and animal health, but also causes huge economic losses and disrupts social order. Seasonal flu causes 3 to 5 million cases of severe illness and 250,000 to 500,000 deaths each year. Influenza viruses can be divided into three types: A, B, and C. Influenza A virus is the main factor that causes seasonal influenza in humans, and it can also infect many animals including poultry, pigs, and horses. Influenza A virus can be divided into many subtypes according to the different structures of surface hemagglutinin (HA) and neuraminidase (NA) antigens. So far, 18 different HA subtypes have been found, among which H1 and H3 are responsible for most of human infl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/75A61P31/16G01N30/02G01N30/06G01N30/34A61K125/00
CPCA61K36/75A61K2236/35A61K2236/39A61K2236/51A61K2236/55A61P31/16G01N30/02G01N30/06G01N30/34
Inventor 王磊刘贵栋
Owner HARBIN VOCATIONAL & TECHN COLLEGE
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