Method for preparing tumor cell vaccine based on non-thermal plasma

A low-temperature plasma and tumor cell vaccine technology, applied in the field of plasma tumor cell vaccine preparation

Pending Publication Date: 2020-06-05
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Malignant tumors, namely cancer, are one of the major diseases facing the world. Existing cancer treatment methods, such as radiotherapy, surgery, and targeted therapy, have certain curative effects, but they also have relatively large limitations.

Method used

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  • Method for preparing tumor cell vaccine based on non-thermal plasma
  • Method for preparing tumor cell vaccine based on non-thermal plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Cell Counting Kit-8 (CCK-8 kit) detects the killing effect of low temperature plasma on cancer cells

[0028] The dielectric barrier discharge low-temperature plasma device is adopted, and the device mainly includes two parts: a high-voltage power supply and a plasma reaction chamber. Wherein, the plasma reaction chamber is composed of 4 pairs of electrodes, and helium is selected as the working gas in all the embodiments of the present application.

[0029] The mouse melanoma cell B16-F10-OVA was used as the test object (purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences). %CO 2 Under certain conditions, culture in DMEM high-glucose medium containing 10% fetal bovine serum and 1% penicillin-streptomycin, and subculture when the cell fusion degree reaches 80%-90%.

[0030] Take the cells growing in the logarithmic phase, wash with preheated PBS, add 1.0 mL of trypsin, centrifuge at 2000 rpm for 5 minutes,...

Embodiment 2

[0033] Example 2 In vivo experiments verify the effectiveness of tumor cell vaccines

[0034] The low-temperature plasma device, experimental materials and low-temperature plasma treatment method are the same as those in Example 1. After low-temperature plasma treatment for 120 seconds, the mouse melanoma cell B16-F10-OVA was cultured for 12 hours, trypsinized, centrifuged at 2000 rpm for 5 minutes, and the cells were collected. Wash the cells with pre-cooled PBS, resuspend and count, and adjust the concentration to 10 7 cells per milliliter. Take 20 C57BL / 6 mice aged 6-8 weeks, and divide them into 2 groups, 10 mice in each group, which are the control group and the experimental group, respectively. On the first day, the mice in the control group were injected intraperitoneally with PBS group (100ul), and the mice in the experimental group were injected intraperitoneally with low-temperature plasma inactivated tumor cells (100ul, about 1-3×10 6 cell). On the third day, th...

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Abstract

The invention discloses a method for preparing a tumor cell vaccine based on non-thermal plasma, and belongs to the field of biomedicine. According to the method, it is found that the non-thermal plasma can significantly kill tumor cells, such as melanoma cells; and injection of tumor cells inactivated by the non-thermal plasma can effectively inhibit tumor growth in vivo, or the non-thermal plasma can be used for direct irradiation on in-situ tumors so that the occurrence and development of metastatic tumors can be effectively inhibited, and new ideas are opened up for the subsequent application of the non-thermal plasma in the field of cancer treatment.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for preparing a low-temperature plasma-based tumor cell vaccine. Background technique [0002] Malignant tumors, namely cancer, are one of the major diseases facing the world. Existing cancer treatment methods, such as radiotherapy, surgery, and targeted therapy, have certain curative effects, but they also have relatively large limitations. Tumor vaccines are one of the research hotspots in cancer treatment in recent years. By introducing tumor vaccines into patients, they can enhance immunogenicity, activate the immune system, and induce the body's cellular and humoral immune responses, thereby controlling tumor growth and metastasis or eliminating tumors. Tumor vaccines mainly include four types: cellular vaccines, peptide vaccines, genetic engineering vaccines, and antibody tumor vaccines. At present, the methods of obtaining tumor cell vaccines are mainly throu...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61P35/00
CPCA61K39/0011A61P35/00A61K2039/585A61K2039/876A61K2039/812A61K2039/892
Inventor 韩伟马洁陈国栋程诚聂莉莉
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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