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A new genus of endophytic fungi tk815 and its application

A technology of TK815 and bacterial strains, applied in the field of microorganisms

Active Publication Date: 2020-10-27
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] The applicant has a very in-depth research on endophytic fungi, and has been constantly exploring new endophytic fungi, for example, the endophytic fungus with the application number 201410220964.7 "A DSE strain and its application in sugarcane production", which was produced by The applicant is isolated from mangrove plants in the Beibu Gulf of Guangxi, belongs to the genus Devriesia, and has the effect of promoting the growth of Dendrobium officinale; the endophytic fungus whose application number is 201610652927.2 "Endophytic fungus L-14 for the prevention and treatment of banana wilt and its application", The fungus was obtained by trapping and separating from the soil by the applicant, belongs to the genus Schizothecium, and has the effect of preventing and treating Fusarium wilt of banana; the application number is 201410220965.1 "DSE strain 24L-4 and its application in the production of Dendrobium officinale" endophytic fungus, the The fungus was isolated and obtained by the applicant from the leaf tissue of camellia, and belongs to the genus Devriesia; at the same time, the line contains many endophytic fungi found by other applicants, such as application number: 201410220965.1 "DSE strain 24L-4 and its effect on the production of Dendrobium officinale "Application" has announced the application of a dark endophytic fungus in promoting the growth of Dendrobium officinale, but the microorganism announced in 201710833508.3 is a certain species of the genus Zasmidium sp. and the microorganism announced in 201410220965.1 is A certain species (Devriesia sp.) of the genus Devriesia sp.; due to the good mutualistic symbiotic relationship between endophytic fungi and the host, research on it has not stopped at present, and there are many new endophytic species that have not been recognized and discovered by us in nature Fungi, in order to enrich the germplasm resources of endophytic fungi, it is necessary to carry out the research of this application

Method used

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  • A new genus of endophytic fungi tk815 and its application
  • A new genus of endophytic fungi tk815 and its application
  • A new genus of endophytic fungi tk815 and its application

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Embodiment 1

[0023] Carry out morphological identification and molecular biology identification to the new genus bacterial strain Tiankengomelania guangxiense TK815 described in the present invention, specifically as follows:

[0024] (1) Morphological identification:

[0025] Such as figure 2 As shown in part A of , the observation of the colony morphology of the strain is as follows: the colony grows slowly on the CMMY medium (wort medium), and the diameter of the colony is 12-15mm after two weeks of cultivation at 28°C. The colonies are nearly round, dark gray-black, raised, with radial grooves.

[0026] Such as figure 2 As shown in Part B-I of the strain, the hyphae of this strain are smooth, septated, light brown-green, and 1.6-3.4 μm wide. Conidiophores erect, separated, unbranched or 1 branched, slightly thicker than hyphae, light brownish green, with sporulation scars, 4.6-87.8×1.7-3μm. Conidia clavate, surface rough, blunt rounded at the end, truncate at the base, light brow...

Embodiment 2

[0031] The isolation method of embodiment 1 bacterial strain is as follows:

[0032] (1) Sampling

[0033]Sampling the soil of Baidong Tiankeng (24°49′2.95″N, 106°27′22.32″E, 1175.7m above sea level) in Dashiwei Tiankeng Group, Leye County, Baise City, Guangxi, and collected the Dashiwei Tiankeng Group by five-point sampling method The rhizosphere soil of primeval forest plants was sampled at a distance of 5-15cm from the soil surface. Soil samples were processed as soon as possible after collection or placed in a 4°C refrigerator for later use.

[0034] S1: Configure separation medium and purification medium:

[0035] The preparation method of the separation medium (1 / 2 cornflour medium) of the present application is: weigh 8.5g of cornflour agar, 15g of agar powder, and finally make up distilled water to settle to 1 000mL and mix;

[0036] The preparation method of the purified culture medium (CMMY medium) of the present application is: weigh 10 g of malt juice agar, 8.5 ...

Embodiment 3

[0038] Embodiment 3: Research on the growth promotion of plants by bacterial strain TK815

[0039] (1) The situation detection of promoting the growth of Dendrobium officinale:

[0040] (1) Growth-promoting test of TK815 on tissue culture seedlings of Dendrobium officinale:

[0041] The strain TK815 was activated and inoculated on 3 oat medium (OMA, g / L, oat flour 10.0, agar 14.0, MgSO 4 ·7H 2 O 1.0, KH 2 PO 4 1.5, NaNO 3 1.0), 3 levels, each named as: TK815-1, TK815-2, TK815-3, inoculate 3 bacterial blocks in each dish, and wait for the bacterial colony to grow up for later use. Select aseptic Dendrobium officinale seedlings with basically the same plant height and robustness and transplant them to the colony, transplant one sterile Dendrobium officinale seedling on each colony, 3 plants per dish, and put the petri dish into a tissue culture bottle , at 26°C, light intensity 180μmol / m 2 ·s 2 , cultured in an artificial climate box with a light time of 16h / d. The dr...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a new genus of endophytic fungus TK815 and its application. The endophytic fungus TK815 of the present invention is a new strain different from the prior art at present, and is found in the soil of Baidong Tiankeng, Dashiwei Tiankeng Group, Leye County, Baise City, Guangxi. TK815 is a new genus of the Herpotrichiellaceae family. The genus of microorganisms is named Tiankengomelania guangxiense, and strain TK815 is the type strain of this genus; the discovery of this new genus of microorganisms enriches the available microbial resources of endophytic fungi, and this strain has a good growth-promoting effect on Dendrobium officinale.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganisms, in particular to a new genus of endophytic fungus TK815 and its application. 【Background technique】 [0002] Endophytic fungi (Endophytic fungi) widely exist in healthy plant tissues and organs, with extremely rich species diversity and functional diversity. As a class of microbial resources that have not been fully studied, endophytic fungi resources are of various types, including many rare and special (or new taxa) strains, and their ecological functions have gradually been discovered and revealed. Studies have shown that endophytic fungi can co-exist with hosts, endow plants with excellent growth traits, improve the host's ability to resist diseases and insect pests and stress resistance in stress environments, thereby reducing the use of chemical fertilizers and pesticides. This kind of fungi has the characteristics of wide ecological distribution, wide host range and isolation and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02A01N63/30A01P21/00C12R1/645
CPCC12N1/02C12N1/14C12N1/145C12R2001/645
Inventor 谢玲龙艳艳张艳张雯龙农倩陈艳露廖仕同覃丽萍
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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