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Small and dense low-density lipoprotein cholesterol assay kit and application thereof

A low-density lipoprotein and kit technology, which is applied in the field of small and dense low-density lipoprotein cholesterol assay kits, can solve the problems of low detection accuracy and precision, high price, and poor stability, and achieve high accuracy, The effect of low production cost and increased accuracy

Active Publication Date: 2020-06-12
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the problems of poor kit stability, low detection accuracy and precision, and relatively expensive price in the process of determining sd LDL-C content in the prior art, a small and dense low-density lipoprotein cholesterol determination kit is provided And its application, the kit significantly improves the stability of the kit by controlling the type and concentration ratio of the surfactant in the reagent, and significantly improves the accuracy and precision of the detection, and reduces the cost of the kit

Method used

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  • Small and dense low-density lipoprotein cholesterol assay kit and application thereof
  • Small and dense low-density lipoprotein cholesterol assay kit and application thereof
  • Small and dense low-density lipoprotein cholesterol assay kit and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0054] The components and concentrations of the small and dense low-density lipoprotein cholesterol assay kit of the present embodiment are as follows:

[0055] Reagent R1 includes: sodium cocoyl isethionate 80mmol / L, cetyl isopropyl ester 20mmol / L, cholesterol esterase 1500U / L, cholesterol oxidase 1000U / L, peroxidase 1500KU / L, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylanilide sodium 1mmol / L and 4-hydroxyethylpiperazineethanesulfonic acid 50mmol / L of pH=7.0;

[0056] Reagent R2 includes: Triton X-100 80mmol / L, peroxidase 5000U / L, 4-aminoantipyrine 1mmol / L, polyoxyethylene lauryl ether 2mmol / L, hydroxypropyl methylcellulose 5mol / L and 4-hydroxyethylpiperazineethanesulfonic acid 50mmol / L with pH=7.0.

Embodiment 2

[0058] The components and concentrations of the small and dense low-density lipoprotein cholesterol assay kit of the present embodiment are as follows:

[0059] Reagent R1 includes: sodium cocoyl isethionate 120mmol / L, cetyl isopropyl ester 50mmol / L, cholesterol esterase 2500U / L, cholesterol oxidase 2000U / L, peroxidase 2500KU / L, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium 3mmol / L and 4-hydroxyethylpiperazineethanesulfonic acid 80mmol / L of pH=7.0;

[0060] The reagent R2 includes: Triton X-100 100mmol / L, peroxidase 6500U / L, 4-aminoantipyrine 3mmol / L, polyoxyethylene lauryl ether 5mmol / L, hydroxypropyl methyl 10 mol / L of cellulose and 80 mmol / L of 4-hydroxyethylpiperazineethanesulfonic acid at pH=7.0.

Embodiment 3

[0062] The components and concentrations of the small and dense low-density lipoprotein cholesterol assay kit of the present embodiment are as follows:

[0063] Reagent R1 includes: sodium cocoyl isethionate 90mmol / L, cetyl isopropyl ester 45mmol / L, cholesterol esterase 1800U / L, cholesterol oxidase 1600U / L, peroxidase 2200KU / L, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium 1.8mmol / L and 4-hydroxyethylpiperazineethanesulfonic acid 60mmol / L of pH=7.0;

[0064] The reagent R2 includes: Triton X-100 95mmol / L, peroxidase 5600U / L, 4-aminoantipyrine 1.5mmol / L, polyoxyethylene lauryl ether 5mmol / L, hydroxypropyl methyl Base cellulose 5mol / L and 4-hydroxyethylpiperazineethanesulfonic acid 60mmol / L of pH=7.0.

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Abstract

The invention provides a small and dense low-density lipoprotein cholesterol assay kit, and belongs to the technical field of biological detection. The kit comprises a reagent R1 and a reagent R2, wherein the R1 includes a surfactant A, cholesterol esterase, cholesterol oxidase, peroxidase, a color developing agent and a buffer solution; and the R2 is includes a surfactant B, the peroxidase, 4-aminoantipyrine, a stabilizer and a buffer solution. Through controlling specific components of a surfactant in the reagent R1 and a concentration ratio of polyoxyethylene lauryl ether to hydroxypropyl methyl cellulose in the reagent R2, sensitivity and accuracy of the kit are remarkably improved, stability of the kit is increased, the components in the kit are simple and easy to obtain, preparationcost is low, and the kit is beneficial to a wide clinical application.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a small and dense low-density lipoprotein cholesterol assay kit and its application. Background technique [0002] With the improvement of living standards, people's blood lipid levels gradually increased, and the prevalence of dyslipidemia increased significantly. Dyslipidemia characterized by elevated low-density lipoprotein cholesterol (LDL-C) or serum total cholesterol (TC) is an important risk factor for atherosclerotic cardiovascular disease. In recent years, it has been found that small and dense low-density lipoprotein cholesterol (smalland low density lipoprotein cholesterol, sd LDL-C) is more likely to be oxidized than ordinary low-density lipoprotein cholesterol, and its ability to cause atherosclerosis is stronger, and it is more likely to cause coronary artery disease. Atherosclerotic heart disease, one of the risk factors for coronary heart...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60C12Q1/44C12Q1/28C12Q1/26G01N33/92
CPCC12Q1/26C12Q1/28C12Q1/44C12Q1/60C12Q2326/96G01N33/92G01N2333/904
Inventor 章晓庆林耀文
Owner 浙江夸克生物科技有限公司
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