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Biogenic amine coordination imprinting material, biogenic amine fluorescent array sensor, preparation method and application thereof

An array sensor, imprinting material technology, applied in analytical materials, fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem of inability to use biogenic amines to identify and detect at the same time, to expand the detection coverage, the principle of fluorescence response is simple, and the preparation of raw materials Inexpensive and accessible effects

Active Publication Date: 2021-09-17
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional fluorescent probes reported so far can only respond to the sum of biogenic amines or individual biogenic amines, and cannot be applied to the simultaneous identification and detection of different biogenic amines.

Method used

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  • Biogenic amine coordination imprinting material, biogenic amine fluorescent array sensor, preparation method and application thereof
  • Biogenic amine coordination imprinting material, biogenic amine fluorescent array sensor, preparation method and application thereof
  • Biogenic amine coordination imprinting material, biogenic amine fluorescent array sensor, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1 Tyramide coordination imprinted magnetic material

[0059] Will Fe 3 o 4 @ MPS (100.0mg), MAA (0.03mL, 0.35mmol), VPBA (0.06g, 0.40mmol), EDMA (0.4mL, 2.00mmol) and tyramine (0.013g, 0.09mmol), add 4.0mL acetonitrile in Stir at room temperature. After pre-polymerization for 1 hour, add the initiator AIBN (20.0mg), heat and stir at 70°C under nitrogen protection for 6 hours. After the polymerization reaction, use methanol / formic acid (v / v, 9 / 1 ), deionized water, and methanol to wash the polymerized product, magnetically separate and remove unreacted monomers and templates, and finally place the prepared tyramide-imprinted magnetic nanoparticles in a vacuum drying oven and dry at 55°C for 12 hours. Depend on figure 1 It can be seen that the prepared imprinted magnetic nanoparticles have a diameter of about 10 nm, and the surface of the magnetic nanoparticles is covered by the imprinted polymer.

Embodiment 2

[0060] Example 2 Histamine coordination imprinted magnetic material

[0061] The difference from Example 1 is that tyramine is replaced with histamine, and the amount of histamine added is 0.01 g. The histamine coordination imprinted magnetic nanoparticles were prepared.

Embodiment 3

[0062] The recognition detection of embodiment 3 tyramine and histamine

[0063] Two imprinted magnetic nanoparticles synthesized in Examples 1 and 2 were used as recognition elements, and 1-aminopyrene was used as a fluorescent indicator to construct a dual-channel fluorescent sensor. The specific detection steps are as follows:

[0064] (1) Prepare a 1 μg / mL 1-aminopyrene solution in acetonitrile, and then dilute it to 0.001 μg / mL with water to obtain a fluorescent indicator solution.

[0065] (2) Mix 5 mg of the tyramide-imprinted magnetic nanoparticles of Example 1 with the fluorescent indicator 1-aminopyrene solution (100 μL, 0.001 μg / mL) to prepare the sensor unit A1;

[0066] 5 mg of histamine-imprinted magnetic nanoparticles in Example 2 were mixed with a fluorescent indicator 1-aminopyrene solution (100 μL, 0.001 μg / mL) to prepare sensor unit A2.

[0067] (3) Prepare a series of tyramine aqueous solutions and histamine aqueous solutions with different concentrations...

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Abstract

The invention discloses a biogenic amine coordination imprinting material, a biogenic amine fluorescence array sensor, a preparation method and application thereof. The biogenic amine coordination imprinting material contains boric acid groups, and the imprinting material can coordinate with the biogenic amine, and the imprinting material can also coordinate with the fluorescent indicator, and the biogenic amine and the fluorescent indicator compete with the imprinting material for coordination respectively. The biogenic amine coordination imprinting material can be used to detect biogenic amines, and is preferably used in biogenic amine fluorescent array sensors. The fluorescent array sensor provided by the present invention uses the coordination imprinting competitive adsorption method for the first time, which can generate fingerprint responses to different biogenic amines, and can realize the simultaneous identification and detection of various biogenic amines, greatly expanding the detection coverage of biogenic amines, and providing a A universal fluorescence detection method for biogenic amines.

Description

technical field [0001] The invention belongs to the field of fluorescent sensor materials, and in particular relates to a biogenic amine coordination imprinting material, a fluorescent array sensor, a preparation method and application thereof. Background technique [0002] Biogenic amines are a class of organic small molecular bases containing reactive nitrogen in their structures, usually produced from amino acid degradation caused by high temperature or bacterial enzymolysis during storage and processing of protein-rich seafood, fish, and fermented foods. Common biogenic amines in food mainly include tyramine, histamine, putrescine, cadaverine, spermine, ethanolamine, etc. It has been reported in the literature that many biogenic amines can cause adverse effects on human health, so these substances have become food quality and safety standards. important biomarkers. In addition, biogenic amines usually exist in metabolic pathways at low concentrations, and play a variety...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6439
Inventor 李楠李耘邱静钱永忠
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS