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Method for producing egg yolk antibody for preventing or treating calf digestive diseases and egg yolk antibody produced thereby and the use thereof

A technology for digestive system diseases and yolk antibodies, which is applied in the field of production methods and the resulting yolk antibodies and their uses, can solve unknowable problems and achieve the effect of preventing or treating calf digestive system diseases

Pending Publication Date: 2020-06-30
郑洪杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, methods for utilizing antigen-antibody complexes for mass production of yolk antibodies have not been known so far

Method used

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  • Method for producing egg yolk antibody for preventing or treating calf digestive diseases and egg yolk antibody produced thereby and the use thereof
  • Method for producing egg yolk antibody for preventing or treating calf digestive diseases and egg yolk antibody produced thereby and the use thereof
  • Method for producing egg yolk antibody for preventing or treating calf digestive diseases and egg yolk antibody produced thereby and the use thereof

Examples

Experimental program
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Effect test

manufacture example 1

[0050] Production Example 1: Production of Antigen

[0051] 1) E. coli (09: K35, K99, F41) KVCC-BA0000540-020800491

[0052] Aerobic culture was carried out in a 37° C. incubator using a blood agar medium. After confirming the colony (Colony), inoculate it in 1000ml BHI broth (brain heart infusion broth), and then culture it with shaking for 24 hours to 36 hours. After the culture was completed, it was treated with 0.3% formalin at room temperature for 48 hours to inactivate it. The inactivated antigen was centrifuged at 6000rpm and recovered. After dispersing the recovered antigen with PBS (phosphate buffered saline, pH 7.2), 0.1% formalin (Formalin) was added and left to stand at room temperature for 1 day, and then stored in a refrigerator at 4°C. The antigen was calculated and the concentration was adjusted so that the OD (optical density) at 410 nm of the produced antigen became 1.0 by a spectrophotometer.

[0053] 2) Rotavirus (KVCC-VR9200180, KVCC-VR9200179, KVCC-...

manufacture example 2

[0056] Production Example 2: Production of Mouse Antibody

[0057] The various antigens produced in Production Example 1 above were inactivated to suppress toxicity, and stored in cold storage before use. In the case of using a mouse antibody to prepare a vaccine, a complete adjuvant (complete adjuvant) is used to prepare the vaccine. Various vaccines corresponding to various antigens are produced, and antibodies are produced according to the usual method for producing mouse antibodies. Mouse antibodies were purified from serum (Serum) recovered after production using a protein-A column.

manufacture example 3

[0058] Production Example 3: Production of a sample that binds to a calf antigen (mAb complex)

[0059] In order to allow the antigen produced in Production Example 1 to bind to the mouse antibody produced in Production Example 2, the following tests were carried out. 100 μg of mouse antibodies produced against various antigens were mixed with the inactivated vaccine mixture, and the antigen-antibody binding was induced overnight at 4°C. The sample in which binding was completed was collected by centrifugation, and used as a sample for egg-laying hen inoculation.

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Abstract

The present invention relates to: a method for producing an egg yolk antibody for preventing or treating calf digestive diseases, which comprises producing a heterologous antibody by using a calf digestive disease-causing bacterium or a viral antigen, and then inoculating a laying chicken with a complex that binds the antigen and the heterologous antibody, thereby producing the yolk antibody; theegg yolk antibody produced thereby and the use thereof. According to the invention, the method for producing an egg yolk antibody for preventing or treating calf digestive system diseases realizes mass production and production of an egg yolk antibody by injecting an antigen-heterologous antibody complex into an egg laying chicken, the egg yolk antibody produced thereby has high specificity and binding properties with respect to calf digestive system disease pathogenic bacteria or virus, and can effectively inhibit the growth of the bacteria and virus, thereby preventing or treatment calf digestive system diseases.

Description

technical field [0001] The present invention relates to the production method of egg yolk antibody for the prevention or treatment of calf digestive system diseases, the egg yolk antibody produced thereby and its use, and more specifically relates to the production of heteroantibody (heteroantibody) by using pathogenic bacteria or virus antigens of calf digestive system diseases , and then inoculate the complex combined with the above-mentioned antigen and heterologous antibody to laying hens, thereby producing egg yolk antibody for the prevention and treatment of calf digestive system diseases, the production method of egg yolk antibody and the thus produced egg yolk antibody and its use . Background technique [0002] Antibody is a globulin protein formed in lymphoid tissue based on B lymphocytes or B cells, and is an important substance involved in specific immune recognition. Antibodies have two different functions. The first is to specifically recognize and bind to a s...

Claims

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Application Information

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IPC IPC(8): C07K16/02C07K16/12C07K16/10A61K39/40A61K39/42A61P31/04A61P31/14A23K20/195
CPCC07K16/12C07K16/10A61K39/40A61K39/42A61P31/04A61P31/14A23K20/195C07K16/02C07K16/1232A23K20/147A23K50/10C07K2317/76C07K2317/92A61K2039/505
Inventor 郑洪杰
Owner 郑洪杰
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