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A strain of Fusarium verticillium and its application

A fusarium and ring pattern technology, which is applied in the field of forestry and agricultural pest control, can solve the problems of pollution and damage to the ecological environment, difficulty in manual spraying, and high cost of spraying by aircraft

Active Publication Date: 2022-06-07
GUANGDONG ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is aimed at the expansion of the current Chinese fir planting area, and the increasingly rampant damage of the Chinese fir-tip moth, but in the prior art, it is difficult to prevent and control the Chinese fir-tip moth, and it is difficult to manually apply pesticides in the process of chemical pesticide control, and the cost of aircraft spraying Larger, and there is a problem of pollution and destruction of the ecological environment, providing an excellent strain of Fusarium verticillium and its application for sustainable and natural control of the pest

Method used

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  • A strain of Fusarium verticillium and its application
  • A strain of Fusarium verticillium and its application
  • A strain of Fusarium verticillium and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1: Isolation and identification of Fusarium parasitizing Fusarium sp.

[0036] The detailed method for obtaining and identifying Fusarium strains is as follows:

[0037] S1. Obtainment of Fusarium strains: The fusarium sclerotiorum was killed by Fusarium infection under natural conditions in Xiaokeng Forest Farm, Qujiang District, Shaoguan City ( figure 1 ) back to the laboratory, and promptly isolate and cultivate the fungi on the surface of the worms.

[0038] S2. Laboratory culture and morphological identification of strains: prepare PDA medium: 200g of potatoes, washed, peeled and chopped, boiled with water for 30min, filtered with gauze, added with 40g of glucose and 18g of agar, dilute to 1L with deionized water, wait for After fully dissolving, it was divided into 250mL conical flasks, each bottle was about 100mL, sealed with silica gel plugs, sterilized at 121°C for 20min, taken out, inoculated after cooling, and cultured on PDA medium for 7 days. Unde...

Embodiment 2

[0052] Example 2: Preparation of Fusarium verticillium FCPC-L01 strain spore suspension

[0053] The preparation method of the spore suspension of Fusarium verticillium FCPC-L01 strain is as follows:

[0054] Take the Fusarium rotundus FCPC-L01 strain, inoculate it on a PDA plate with an inoculation loop under aseptic conditions for activation, and cultivate for 7 days in a constant temperature incubator at 25°C and light: dark (12L:12D) conditions. After the mycelium has covered the entire petri dish, add 20 mL of sterile water containing 0.03% Tween-80 by mass, gently scrape the mycelium and spores with an inoculating needle, pour the bacterial liquid into a beaker, and filter the bacteria with double-layer medical gauze. The solution was stirred with a magnetic stirrer for 10 min to obtain a conidia suspension. Calculate the spore concentration of the spore suspension with a hemocytometer, and dilute it to 1×10 with sterile water with a mass fraction of 0.05% Tween-80 6 S...

Embodiment 3

[0055] Example 3: Screening of the best formula for liquid culture medium

[0056] Taking the spore suspension of the Fusarium verticillium FCPC-L01 bacterial strain prepared in Example 2 as the seed liquid, fermentation was carried out in the following five kinds of substratum, and the sporulation capacity of this bacterial strain in different substratum was compared:

[0057] No. 1 culture solution (glucose potato culture solution PD): take 200g of potatoes, wash, peel and chop, add water to boil for 30min, filter with gauze, add 40g of glucose to the filtrate, and dilute to 1L with deionized water. Dispense into 250mL conical flasks, 100mL per bottle, seal with silica gel plug, natural pH value, sterilize at 121°C for 20min, take out, cool and store for later use.

[0058] No. 2 culture solution (sucrose potato culture solution PS): take 200g of potatoes, wash, peel and chop, add water to boil for 30min, filter with gauze, add 40g of sucrose to the filtrate, and dilute to 1...

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Abstract

The invention discloses a strain of Fusarium verticillium and its application. Fusarium concentricum FCPC‑L01 strain (Fusarium concentricum FCPC‑L01) of the present invention has a preservation number of: GDMCC No: 60971; its optimal liquid fermentation condition is: sucrose potato culture broth PS, and the temperature of fermentation culture is 25°C ‑28°C, initial pH 4‑6, shaker speed 180‑210rpm; transfer the fermented broth to rice for solid fermentation, and the conidia produced are highly toxic to the larvae of Toritoria chinensis. The invention provides a biocontrol strain and a fermentation method for preventing and controlling the major pest of Chinese fir, the torch moth, which is more green, environmentally friendly, non-toxic and safe, and has important Market application and promotion value.

Description

technical field [0001] The invention belongs to the technical field of using insect-borne fungi to control forestry and agricultural pests, and in particular relates to a strain of Fusarium verticillium and its application. Background technique [0002] Chinese fir is an endemic species in China. It grows quickly, is easy to survive, and has good wood properties. It is the main fast-growing wood species in southern provinces. Chinese fir seed orchards are a base for providing excellent seed sources. According to statistics, in 1987, the area of ​​Chinese fir improved seed bases in our province exceeded 4,000 mu, which played a positive role in promoting the improvement of Chinese fir seeds and rapid growth and high yield. The outbreak of Chinese fir diseases and insect pests is one of the important factors affecting seed yield and sowing quality. Therefore, pest control is one of the important contents of the management of Chinese fir seed gardens. Polychrosis cunninhamiac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/30A01P7/04C12R1/77
CPCC12N1/14C12N3/00C12R2001/77C12N1/145
Inventor 邱华龙秦长生练涛田龙艳张伟叶真任徐金柱赵丹阳杨华靳秀芳陆建康
Owner GUANGDONG ACAD OF FORESTRY
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