Alkaloid compound extracted from orychophragmus violaceus seeds and extraction method and application thereof
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A technology of alkaloids and extraction methods, applied in the field of natural medicinal chemistry, can solve the problems of low structural system, only a few, unsystematic, etc.
Active Publication Date: 2020-07-07
ACADEMY OF MILITARY MEDICAL SCI
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Problems solved by technology
[0005] At present, most of the compounds isolated from the seeds of Zhuge vegetable are known, and their structure is relatively low (systematic structure means that there are not only a few compounds of each type in a plant, but should be systematic , the number is large, and the system is low, which means that there are only a few components of each type, which is not systematic)
It shows that a large number of compounds in this plant have not been isolated and identified, reflecting that this plant has not been fully developed and utilized, so the separation and development of new compounds in Zhuge Cai are urgently needed
Method used
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Effect test
Embodiment 1
[0058] Step 1: Weigh 40kg of dried Zhugecai seeds with a water content of 5%, and use 240kg of ethanol water (ethanol:water (volume ratio)=7:3) mixed solvent to extract at 78°C under reflux, 3 times for 2h each time , recover ethanol under reduced pressure, put it at room temperature, and obtain the refluxed extract for later use.
[0059] Step 2: adding an equal volume of n-butanol to the reflux extract obtained in step 1 and extracting repeatedly 7 times, recovering n-butanol under reduced pressure to the extract to obtain n-butanol extract.
[0060] Step 3: The n-butanol extract obtained in step 2 is separated by column chromatography on a silica gel column, wherein the silica gel is 200-300 mesh, and sequentially eluted with chloroform-methanol gradient (gradient is 100:0, 50:1, 20︰1, 10︰1, 5︰1, 1︰1), 10 column volumes for each gradient; detected by thin-layer chromatography, ethanol-concentrated sulfuric acid was heated to develop color, and the combined chromatographic b...
Embodiment 2
[0067] Step 1-step 4, step 6: same as embodiment one.
[0068] Step 5: Same as Example 1, only the volume ratio of chloroform-ethyl acetate-methanol is 1:5:5.
[0069] The normalization method determined the purity to be higher than 98%.
Embodiment 3
[0071] Step 1-step 4, step 6: same as embodiment one.
[0072] Step 5: Same as Example 1, only the volume ratio of chloroform-ethyl acetate-methanol is 1:1:3.
[0073] The normalization method determined the purity to be higher than 98%.
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Abstract
The invention discloses an alkaloid compound extracted from orychophragmus violaceus seeds and an extraction method and application of the alkaloid compound. The alkaloid compound is extracted from orychophragmus violaceus seeds, and the chemical structural formula of the alkaloid compound is shown as a formula (1). The alkaloid compound provided by the invention has a good liver protection effect, can be used as a synthetic primer of other compounds and a raw material for new drug development and pharmacological activity research, and can also be used for preparing liver protection drugs. Inaddition, the extraction method is simple, convenient and rapid, the process method is environment-friendly, and the purity is greater than 98%.
Description
technical field [0001] The invention relates to the technical field of natural medicinal chemistry, in particular to an alkaloid compound extracted from the seeds of Zhugecai and its extraction method and application. Background technique [0002] Orychophragmus violaceus (L.) O.E.Schulz is an annual or perennial herbaceous plant of the family Brassicaceae. It is mainly distributed in central and eastern Asia, and widely distributed in my country. It is also known as Eryuelan. As a folk medicine with a long history, Zhuge Cai has physiological activities such as inhibiting tumors, antibacterial and anti-inflammatory, and protecting the liver. Traditional Chinese medicine has recorded its medicinal properties since ancient times: "Warm in nature, pungent in taste, bitter, sweet, enters the stomach meridian". "Medicine Properties" discusses that the plant has the effects of warming the spleen and appetizing, digesting food, lowering qi and diuresis. [0003] Modern clinical ...
Claims
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