Method for recovering D-p-hydroxyphenylglycine from D-p-hydroxyphenylglycine crystallization mother liquor synthesized by an enzymic method
A p-hydroxyphenylglycine, recovery method technology, applied in the direction of organic chemistry methods, chemical instruments and methods, preparation of organic compounds, etc., can solve the problems of poor product purity, low concentration multiple, high energy consumption, etc., and meet high assembly requirements , the effect of guaranteed yield
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Embodiment 1
[0036] Reclaim enzymatic synthesis of D-p-hydroxyphenylglycine in the mother liquor of D-p-hydroxyphenylglycine crystallization according to the following steps:
[0037] (1) Adsorption of D-p-hydroxyphenylglycine by macroporous adsorption resin
[0038] Resin activation: macroporous adsorption resin (LXT-053 resin from Xi'an Lanxiao Technology Co., Ltd.) soaked in methanol for 5 hours, filtered, and then rinsed with purified water to clean the resin surface, and then wet-packed, cylindrical column bed , the resin loading capacity is 900mL, and the aspect ratio is 8;
[0039] D-hydroxyphenylglycine adsorption: take 50L of D-hydroxyphenylglycine crystallization mother liquor, wherein the concentration of D-hydroxyphenylglycine is 23.54g / L, the conductivity is 118ms / cm, and the pH value is 4.9; use 20wt% hydrochloric acid The solution adjusts the pH of the mother liquor to 2±0.2, and then passes through the cylindrical resin column bed prepared above at a flow rate of 35L per h...
Embodiment 2
[0049] Reclaim enzymatic synthesis of D-p-hydroxyphenylglycine in the mother liquor of D-p-hydroxyphenylglycine crystallization according to the following steps:
[0050] (1) Adsorption of D-p-hydroxyphenylglycine by macroporous adsorption resin
[0051] Resin activation: macroporous adsorption resin (LXT-053 resin from Xi'an Lanxiao Technology Co., Ltd.) soaked in methanol for 5 hours, filtered, and then rinsed with purified water to clean the resin surface, and then wet-packed, cylindrical column bed , the resin loading capacity is 900mL, and the aspect ratio is 8;
[0052] D-hydroxyphenylglycine adsorption: Take 50L of D-p-hydroxyphenylglycine crystallization mother liquor, wherein the concentration of D-hydroxyphenylglycine is 26.37g / L, the conductivity is 118ms / cm, and the pH value is 5.1; use 20wt% hydrochloric acid The solution adjusts the pH of the mother liquor to 2±0.2, and then passes through the cylindrical resin column bed prepared above at a flow rate of 34L per...
Embodiment 3
[0061] Embodiment 3 (pilot test)
[0062] Reclaim enzymatic synthesis of D-p-hydroxyphenylglycine in the mother liquor of D-p-hydroxyphenylglycine crystallization according to the following steps:
[0063] (1) Adsorption of D-p-hydroxyphenylglycine by macroporous adsorption resin
[0064] Resin activation: macroporous adsorption resin (LXT-053 resin from Xi'an Lanxiao Technology Co., Ltd.) soaked in methanol for 5 hours, filtered, and then rinsed with purified water to clean the resin surface, and then wet-packed, cylindrical column bed , the resin loading capacity is 36L, and the aspect ratio is 8;
[0065] D-hydroxyphenylglycine adsorption: Take 2000L of D-hydroxyphenylglycine crystallization mother liquor, wherein the concentration of D-hydroxyphenylglycine is 21.92g / L, the conductivity is 119.5ms / cm, and the pH value is 5.1; use 20wt% Hydrochloric acid solution adjusts the pH of the mother liquor to 2±0.2, then passes through the above-mentioned cylindrical resin column ...
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