Continuously integrated respiratory infection virus self-detection kit and application thereof

Abstract

The invention discloses a continuously integrated respiratory infection virus self-detection kit, which comprises a sampling tube and a splitting-amplifying tube, wherein one end of the sampling tubeis provided with an opening, and the other end is sealed through a sieve membrane; an absorption filtration carrier is arranged in the sampling tube; and a buffer solution for splitting media, nucleicacid amplification media and nucleic acid fluorescent dyes is pre-loaded in the splitting-amplifying tube. The invention also discloses application of the kit, the kit is applied to the detection ofrespiratory infection viruses, and a detected person can finish continuously integrated respiratory infection virus self-detection on the own. The aerosol of the nasal cavity can be directly collectedto finish nucleic acid extraction and enrichment, nucleic acid purification and amplification and nucleic acid visual detection in an aerosol sample in one step, operation programs are greatly simplified, minimum duration for obtaining two visual detection results or above is 15 min, and therefore, the kit disclosed by the invention is especially suitable for the safe and quick field screening ofvirus nucleic acid in places, including customs, epidemic area checkpoints and the like.

Description

technical field [0001] The invention relates to the technical field of biological nucleic acid detection, in particular to a continuous and integrated self-detection kit for respiratory infectious virus and its application. Background technique [0002] For respiratory infectious viruses, the swab sampling method is a commonly used virus collection strategy at present; the traditional swab sampling detection method is caused by the defects of poor sample validity, high technical requirements, long processing process, etc., and the swab detection procedure of batch centralized processing. The risk of secondary spread of the virus in a small space cannot be applied to the on-site rapid screening of respiratory infectious viruses. Important occasions such as high-incidence areas and epidemic prevention checkpoints put forward extremely high requirements for on-site rapid screening of respiratory infectious viruses: [0003] (1) The operating threshold needs to be significantly...

AI Technical Summary

Problems solved by technology

The traditional respiratory aerosol virus nucleic acid detection operation involves three main procedures: swab sampling, sample processing (including cell lysis and digestion, nucleic acid extraction and enrichment, polymerase chain reaction (PCR) nucleic acid amplification, etc.), instrument detection, Except for swab sampling, other procedures need to be implemented by professionals under professional conditions. At the same time, it is necessary to ensure the ventilation, sterilization, and cleaning conditions of the implementation environment, which is not conducive to implementation in places with dense population flow, such as airports, checkpoints, and customs. Wait

[0004] (2) Screening procedures require security

For RNA viruses, the time required for the first round of RT-PCR is nearly 60 minutes, and the time scale required for multiple nested RT-PCR amplification is even greater

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