Application of Enshi Ampelopsis grossedentata alcohol extract to restraining of putridity of high-temperature boiled fish meat
A technology for ethanol extraction and Enshi vine tea, which is applied in the preservation of meat/fish with chemicals, etc., can solve the problems of affecting the flavor and quality of fish meat, restricting large-scale application, and high extraction process requirements, so as to improve nutritional value and food safety. , the effect of slowing down the chain reaction and simple extraction process
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Embodiment 1
[0018] Embodiment 1 The application of the ethanol extract of Enshi vine tea of the present invention in inhibiting the corruption of high-temperature cooked fish meat,
[0019] Each raw material used in the following examples and equipment source and detection method are as follows:
[0020] 1 Materials and methods
[0021] 1.1 Materials and equipment
[0022] DFY-1000 Chinese herbal medicine mill (Wenling Linda Machinery Co., Ltd.), imark microplate reader (Bio-Rad, USA), RE-200A rotary evaporator (Guangzhou Xingshuo Instrument Co., Ltd.), hermo Fisher conductivity instrument (model: 3-star), CR-80CO2 incubator (Guangzhou Kangheng Instrument Co., Ltd.), etc.; the rest of the reagents are domestic analytical grade. Three live carps (0.52kg, 0.63kg, 1.1kg) were purchased from Trust-Mart supermarket in Enshi City. The rattan tea samples were collected from the surrounding areas of Enshi City. The senior agronomist of the Institute of Chinese Medicinal Materials of Hubei Aca...
Embodiment 2
[0061] Example 2 The application of the ethanol extract of Enshiteng tea in the present invention in inhibiting spoilage of high-temperature cooked fish, the application of the ethanol extract of Enshiteng tea in inhibiting spoilage of high-temperature cooked fish. Dried rattan tea is crushed, and soaked in 50% ethanol for 17 hours according to the ratio of solid to liquid: 1:40. Power 190w ultrasonic leaching for 28min, reduced pressure and concentration conditions: pressure -0.085MPa, temperature 35°C, the ethanol extract of vine tea was concentrated into extract-like solid, and there was no droplet in the condenser tube of the rotary evaporator, and vine tea ethanol without ethanol solvent was obtained Extract embalming solution. The ethanol extract of vine tea is dissolved in sterile water so that its concentration is 40.0 mg·mL -1 , immerse the cooked fish in a concentration of 40.0mg·mL -1 Soak in the preservative solution for 30min.
Embodiment 3
[0062] Example 3 The application of the ethanol extract of Enshi vine tea in the present invention in inhibiting spoilage of high-temperature cooked fish, the application of the ethanol extract of Enshi vine tea in inhibiting spoilage of high-temperature cooked fish. Dried rattan tea is crushed, and soaked in 70% ethanol for 19 hours according to the ratio of solid to liquid: 1:55. Power 210w ultrasonic leaching for 32min, reduced pressure and concentration conditions: pressure -0.095MPa, temperature 40°C, vine tea ethanol extract was concentrated into extract-like solid, and there was no droplet in the condenser tube of the rotary evaporator, and vine tea ethanol without ethanol solvent was obtained Extract embalming solution. The ethanol extract of vine tea is dissolved in sterile water so that its concentration is 50.0 mg·mL -1 , immerse the cooked fish in a concentration of 50.0mg·mL -1 Soak in the preservative solution for 35 minutes.
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